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Distance_Between_Minima_Tool

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Volker edited this page Jan 29, 2021 · 3 revisions

The tool measures the mean distance between the minima in the profile plot.

image.png plot.png results.png

Getting Started

To install the tool save the file Distance_Between_Minima_Tool.ijm under macros/toolsets in your FIJI installation.

Select the Distance_Between_Minima_Tool from the >> button of the ImageJ launcher.

toolset

Usage

Make a line-selection on the image covering a repetitive structure. Set the line-width of the line selection to cover the width of the structure of interest to smooth the plot. Right-click on the tool-button and adjust the tolerance-option to ignore small local minima. Press the tool-button to measure the mean distance between the minima.

Options

options.png

  • tolerance - the minimum difference needed to separate two minima
  • remove edge minima - if checked minima at the beginning and the end of the selection are excluded
  • show plot - if checked the profile plot with the detected minima is shown

Publications using the tool

Souidi, M., Sleiman, Y., Acimovic, I., Pribyl, J., Charrabi, A., Baecker, V., Scheuermann, V., Pesl, M., Jelinkova, S., Skladal, P., et al. (2021). Oxygen Is an Ambivalent Factor for the Differentiation of Human Pluripotent Stem Cells in Cardiac 2D Monolayer and 3D Cardiac Spheroids. IJMS 22, 662.

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