Skip to content

Commit

Permalink
remove R script ngsupload
Browse files Browse the repository at this point in the history
  • Loading branch information
yeroslaviz committed Nov 28, 2024
1 parent a3762df commit 8e9bc21
Show file tree
Hide file tree
Showing 3 changed files with 15 additions and 52 deletions.
Binary file added docs/htseqvsperseus.table.rar
Binary file not shown.
61 changes: 13 additions & 48 deletions docs/ngsupload.html
Original file line number Diff line number Diff line change
Expand Up @@ -22,40 +22,6 @@
margin: 0 0.8em 0.2em -1em; /* quarto-specific, see https://github.com/quarto-dev/quarto-cli/issues/4556 */
vertical-align: middle;
}
/* CSS for syntax highlighting */
pre > code.sourceCode { white-space: pre; position: relative; }
pre > code.sourceCode > span { line-height: 1.25; }
pre > code.sourceCode > span:empty { height: 1.2em; }
.sourceCode { overflow: visible; }
code.sourceCode > span { color: inherit; text-decoration: inherit; }
div.sourceCode { margin: 1em 0; }
pre.sourceCode { margin: 0; }
@media screen {
div.sourceCode { overflow: auto; }
}
@media print {
pre > code.sourceCode { white-space: pre-wrap; }
pre > code.sourceCode > span { display: inline-block; text-indent: -5em; padding-left: 5em; }
}
pre.numberSource code
{ counter-reset: source-line 0; }
pre.numberSource code > span
{ position: relative; left: -4em; counter-increment: source-line; }
pre.numberSource code > span > a:first-child::before
{ content: counter(source-line);
position: relative; left: -1em; text-align: right; vertical-align: baseline;
border: none; display: inline-block;
-webkit-touch-callout: none; -webkit-user-select: none;
-khtml-user-select: none; -moz-user-select: none;
-ms-user-select: none; user-select: none;
padding: 0 4px; width: 4em;
}
pre.numberSource { margin-left: 3em; padding-left: 4px; }
div.sourceCode
{ }
@media screen {
pre > code.sourceCode > span > a:first-child::before { text-decoration: underline; }
}
/* CSS for citations */
div.csl-bib-body { }
div.csl-entry {
Expand Down Expand Up @@ -476,20 +442,19 @@ <h1 data-number="7"><span class="header-section-number">7</span> Comparison with
</figcaption>
</figure>
</div>
<p><code>HTSeq-count</code> <span class="citation" data-cites="anders2014">(<a href="#ref-anders2014" role="doc-biblioref">Anders, Pyl, and Huber 2014</a>)</span> is part of the HTSeq suite, a python library to facilitate and represent data, such as genomic coordinates, sequences, sequencing reads, alignments, gene model information and variant calls. <code>htseq-count</code> can be used to pre-processes RNA-Seq data for differential expression analysis by counting the overlap of reads with genes. To recreate the plot, one can download the data table below <span class="citation" data-cites="downloadthis">(<a href="#ref-downloadthis" role="doc-biblioref">Mattioni Maturana 2022</a>)</span></p>
<div class="cell">
<div class="sourceCode cell-code" id="cb1"><pre class="sourceCode r code-with-copy"><code class="sourceCode r"><span id="cb1-1"><a href="#cb1-1" aria-hidden="true" tabindex="-1"></a><span class="co"># remotes::install_github("fmmattioni/downloadthis")</span></span>
<span id="cb1-2"><a href="#cb1-2" aria-hidden="true" tabindex="-1"></a><span class="fu">library</span>(downloadthis)</span>
<span id="cb1-3"><a href="#cb1-3" aria-hidden="true" tabindex="-1"></a><span class="fu">download_file</span>(</span>
<span id="cb1-4"><a href="#cb1-4" aria-hidden="true" tabindex="-1"></a> <span class="at">path =</span> <span class="st">"htseqvsperseus.table.rar"</span>,</span>
<span id="cb1-5"><a href="#cb1-5" aria-hidden="true" tabindex="-1"></a> <span class="at">output_name =</span> <span class="st">"HTSeq vs. Persues Table"</span>,</span>
<span id="cb1-6"><a href="#cb1-6" aria-hidden="true" tabindex="-1"></a> <span class="at">button_label =</span> <span class="st">"Download data table"</span>,</span>
<span id="cb1-7"><a href="#cb1-7" aria-hidden="true" tabindex="-1"></a> <span class="at">button_type =</span> <span class="st">"info"</span>,</span>
<span id="cb1-8"><a href="#cb1-8" aria-hidden="true" tabindex="-1"></a> <span class="at">has_icon =</span> <span class="cn">TRUE</span>,</span>
<span id="cb1-9"><a href="#cb1-9" aria-hidden="true" tabindex="-1"></a> <span class="at">icon =</span> <span class="st">"fa fa-save"</span>,</span>
<span id="cb1-10"><a href="#cb1-10" aria-hidden="true" tabindex="-1"></a> <span class="at">self_contained =</span> <span class="cn">FALSE</span></span>
<span id="cb1-11"><a href="#cb1-11" aria-hidden="true" tabindex="-1"></a>)</span></code><button title="Copy to Clipboard" class="code-copy-button"><i class="bi"></i></button></pre></div>
</div>
<p><code>HTSeq-count</code> <span class="citation" data-cites="anders2014">(<a href="#ref-anders2014" role="doc-biblioref">Anders, Pyl, and Huber 2014</a>)</span> is part of the HTSeq suite, a python library to facilitate and represent data, such as genomic coordinates, sequences, sequencing reads, alignments, gene model information and variant calls. <code>htseq-count</code> can be used to pre-processes RNA-Seq data for differential expression analysis by counting the overlap of reads with genes. To recreate the plot, one can download the <a href="htseqvsperseus.table.rar">data table</a> below <span class="citation" data-cites="downloadthis">(<a href="#ref-downloadthis" role="doc-biblioref">Mattioni Maturana 2022</a>)</span></p>
<pre><code>
# remotes::install_github("fmmattioni/downloadthis")
library(downloadthis)
download_file(
path = "htseqvsperseus.table.rar",
output_name = "HTSeq vs. Persues Table",
button_label = "Download data table",
button_type = "info",
has_icon = TRUE,
icon = "fa fa-save",
self_contained = FALSE
)</code></pre>
<!-- ==== Parameters specific for "Chromatin IP" experiment ====
The most popular way to deal with Chromatin IP experiment is sequence relatively small part of a read - enough for unique alignment - and already in silico extend such read until expected length.
{{ :user:activities:matrixupload:chipseqextend.png?500 |}}
Expand Down
6 changes: 2 additions & 4 deletions ngsupload.qmd
Original file line number Diff line number Diff line change
Expand Up @@ -134,11 +134,9 @@ Specifies the number of used threads for uploading NGS data.

![Comparing HTSeq-counts against Perseus](images/htseqvsperseus.multiscatter.png){#fig-htseqvsperseus}

`HTSeq-count` [@anders2014] is part of the HTSeq suite, a python library to facilitate and represent data, such as genomic coordinates, sequences, sequencing reads, alignments, gene model information and variant calls. `htseq-count` can be used to pre-processes RNA-Seq data for differential expression analysis by counting the overlap of reads with genes. To recreate the plot, one can download the data table below [@downloadthis]
`HTSeq-count` [@anders2014] is part of the HTSeq suite, a python library to facilitate and represent data, such as genomic coordinates, sequences, sequencing reads, alignments, gene model information and variant calls. `htseq-count` can be used to pre-processes RNA-Seq data for differential expression analysis by counting the overlap of reads with genes. To recreate the plot, one can download the [data table](htseqvsperseus.table.rar) below [@downloadthis]

```{r}
#| echo: true
#| eval: false
```
# remotes::install_github("fmmattioni/downloadthis")
library(downloadthis)
Expand Down

0 comments on commit 8e9bc21

Please sign in to comment.