SB Reverse transcribe

--reverse_transcribe, -r2d

Description

Convert RNA to cDNA.

Example

Input file: Mle-Panxα2.gb

LOCUS       Mle-Panxα2              1314 bp    RNA              UNA 02-JAN-2015
DEFINITION  cDNA - ML25998a.
ACCESSION   Mle-Panxα2
VERSION     Mle-Panxα2
KEYWORDS    .
SOURCE
  ORGANISM  . . . .
            .
FEATURES             Location/Qualifiers
     CDS             order(1..144,145..307,308..555,556..688,689..810,811..1314)
                     /label
                     /created_by="User"
                     /modified_by="User"
     TMD1            85..147
     splice_donor    298..307
                     /label="Donor"
                     /created_by="User"
     TMD2            421..483
     splice_acceptor complement(495..504)
                     /label="Acceptor"
                     /created_by="User"
     TMD3            682..744
     TMD4            931..1023
ORIGIN
        1 augguauugg aucucauuuc uggaagcuug aauggcuuuu uaaagaucaa gucaguuagc
       61 aucgacgauc agugggacca gauuaacaga accuauuugg ucauguuuug uauuuuaucu
      121 gguacaauca ugaccuuuaa acagaauuua ggaucaauaa uacacuguau aucggaugca
      181 agaggcgacg acaguucguu ugcggaugcu caugcgacau uugugcaaga cuauugugcu
      241 gcucaagggc uguacacuuu aaaagaagug uaugacaagu cuuggccaga ugaaauuccu
      301 uacccaggua uucuccaaau gaaaacaauc gguuguuucc cggggagaca guucaaaaac
      361 ggaaccccca uccagugccc ggacgagaaa gaucugaaac ccuucacaac ggucuaucau
      421 gucugguaca uguucguacc guucuacuuc ugcgcuguug gcaucgcuuu uuacuucccc
      481 uacacgguuu ucagacaccu cagcggcauc uacgacauca agccuauguu gaacagccuu
      541 gcccucgaca uuggggccua cacggaggag gacauaaguc gacguauaga caaugucucg
      601 aggugguugu acaucaaguu ggaucccuac augaacaaca ugcuuccuua uacucagaua
      661 guucacaaac auuccaucuu uuacacggug auguugguga aggugaugua ccuagcuacc
      721 aguguuucua uuuuuuacgc cacucaccgg auauucgacc aaggaaacuu ugcacucuac
      781 ggauacgaug uucuaaugag cauaccacag gaaacaagcu auaaagugau ggacacaauc
      841 uucccuaaaa ugguuggcug ugagaucaac auguggggcc ggacuggcga acagagcgaa
      901 ucucuucugu guguccuccc ucaaaacauc ggcaaccaau acuucuuccu uauauucugg
      961 uuucuccuga uucucaccau acuuuccaac uguaucucug uaauagugac cauauucaga
     1021 uuuauauucg uuagugggag cuacaaaagg uuccuggcua ccagccucuu gaaucacgaa
     1081 gaacgauaca agcugguguu uacacauguc ggcacgacug gaagauacau uuuacugcuc
     1141 ugugccgauc auagcaaccc caaaauauuc gaggaucuuc uagagaucgu cuguucccuu
     1201 cucauagcaa acuaucacaa aagaaagagg agucgggaua agggacacag ucgagcggag
     1261 gggguaggga cuaaagggcg acacggucug ucuuuugugg acucaaccgu guga
//

Usage

$: sb sequences.fa -r2d

Output

LOCUS       Mle-Panxα2              1314 bp    DNA              UNA 02-JAN-2015
DEFINITION  cDNA - ML25998a.
ACCESSION   Mle-Panxα2
VERSION     Mle-Panxα2
KEYWORDS    .
SOURCE
  ORGANISM  . . .
            .
FEATURES             Location/Qualifiers
     CDS             order(1..144,145..307,308..555,556..688,689..810,811..1314)
                     /modified_by="User"
                     /created_by="User"
                     /label
     TMD1            85..147
     splice_donor    298..307
                     /created_by="User"
                     /label="Donor"
     TMD2            421..483
     splice_acceptor complement(495..504)
                     /created_by="User"
                     /label="Acceptor"
     TMD3            682..744
     TMD4            931..1023
ORIGIN
        1 atggtattgg atctcatttc tggaagcttg aatggctttt taaagatcaa gtcagttagc
       61 atcgacgatc agtgggacca gattaacaga acctatttgg tcatgttttg tattttatct
      121 ggtacaatca tgacctttaa acagaattta ggatcaataa tacactgtat atcggatgca
      181 agaggcgacg acagttcgtt tgcggatgct catgcgacat ttgtgcaaga ctattgtgct
      241 gctcaagggc tgtacacttt aaaagaagtg tatgacaagt cttggccaga tgaaattcct
      301 tacccaggta ttctccaaat gaaaacaatc ggttgtttcc cggggagaca gttcaaaaac
      361 ggaaccccca tccagtgccc ggacgagaaa gatctgaaac ccttcacaac ggtctatcat
      421 gtctggtaca tgttcgtacc gttctacttc tgcgctgttg gcatcgcttt ttacttcccc
      481 tacacggttt tcagacacct cagcggcatc tacgacatca agcctatgtt gaacagcctt
      541 gccctcgaca ttggggccta cacggaggag gacataagtc gacgtataga caatgtctcg
      601 aggtggttgt acatcaagtt ggatccctac atgaacaaca tgcttcctta tactcagata
      661 gttcacaaac attccatctt ttacacggtg atgttggtga aggtgatgta cctagctacc
      721 agtgtttcta ttttttacgc cactcaccgg atattcgacc aaggaaactt tgcactctac
      781 ggatacgatg ttctaatgag cataccacag gaaacaagct ataaagtgat ggacacaatc
      841 ttccctaaaa tggttggctg tgagatcaac atgtggggcc ggactggcga acagagcgaa
      901 tctcttctgt gtgtcctccc tcaaaacatc ggcaaccaat acttcttcct tatattctgg
      961 tttctcctga ttctcaccat actttccaac tgtatctctg taatagtgac catattcaga
     1021 tttatattcg ttagtgggag ctacaaaagg ttcctggcta ccagcctctt gaatcacgaa
     1081 gaacgataca agctggtgtt tacacatgtc ggcacgactg gaagatacat tttactgctc
     1141 tgtgccgatc atagcaaccc caaaatattc gaggatcttc tagagatcgt ctgttccctt
     1201 ctcatagcaa actatcacaa aagaaagagg agtcgggata agggacacag tcgagcggag
     1261 ggggtaggga ctaaagggcg acacggtctg tcttttgtgg actcaaccgt gtga
//