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flye update + 0.10
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mikolmogorov committed Oct 29, 2022
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10 changes: 5 additions & 5 deletions README.md
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# HapDup
# Hapdup

HapDup (haplotype duplicator) is a pipeline to convert a haploid long read assembly into a diploid assembly.
HapDup works with either Oxford Nanopore or PacBio (HiFi or CLR). The reconstructed haplotypes preserve heterozygous
structural variants (in addition to small variants) and are locally phased.


## Version 0.8
## Version 0.10

Input requirements
------------------
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```
cd directory_with_assembly_and_alignment
HD_DIR=`pwd`
docker run -v $HD_DIR:$HD_DIR -u `id -u`:`id -g` mkolmogo/hapdup:0.8 \
docker run -v $HD_DIR:$HD_DIR -u `id -u`:`id -g` mkolmogo/hapdup:0.10 \
hapdup --assembly $HD_DIR/assembly.fasta --bam $HD_DIR/lr_mapping.bam --out-dir $HD_DIR/hapdup -t 64 --rtype ont
```

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`--rtype hifi` instead of `--rtype ont`.

```
singularity pull docker://mkolmogo/hapdup:0.8
singularity pull docker://mkolmogo/hapdup:0.10
HD_DIR=`pwd`
singularity exec --bind $HD_DIR hapdup_0.8.sif \
singularity exec --bind $HD_DIR hapdup_0.10.sif \
hapdup --assembly $HD_DIR/assembly.fasta --bam $HD_DIR/lr_mapping.bam --out-dir $HD_DIR/hapdup -t 64 --rtype ont
```

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2 changes: 1 addition & 1 deletion hapdup/__version__.py
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__version__ = 0.8
__version__ = 0.10
2 changes: 1 addition & 1 deletion submodules/Flye
Submodule Flye updated from f2b642 to e1546a

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