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1. What were you trying to do?
I was trying to report coordinates from a linear genome onto the graph using vg inject, and report the coordinates from the graph to a target linear genome using vg surject.
I converted a BED file with my coordinates to a BAM file using bedtools bedtobam. Then I used the BAM file with vg inject to output a GAF file. The GAF file was used by vg surject to output a SAM file.
2. What did you want to happen?
I wanted the resulting SAM file to have the sequence corresponding to my coordinates in the target genome.
3. What actually happened?
When I used vg version 1.58 it worked as expected, but with vg version 1.59 and 1.60, the sequence field in the SAM file has a surprising sequence that doesn't correspond to DNA, and I don't what it corresponds to. The strange sequence in version 1.59 and 1.60 has the same length as the DNA sequence in version 1.58.
When I compared the GAF files from vg inject, it appears the problems comes from these files, so vg inject seems responsible.
Here is an example of the strange sequence in the SAM (field 10) and GAF (field 12) files :
1.58 SAM file : TTTCTTGACCTCTGCTACTGCCATTCTGTACGGCCTCTTGTAAATTGGGGCGGTTCCTGGTGCCAAATCAATCCGGAATTCAATCTCTCTCTTGGGTGGCATAGTAGTGAGGTCCTCAGGGAACACCTCTGGATACTCGCAGACTATGGGGATATCTTCCAATTTCCTCCAACTCTTCTCCTCGGTGACCACCGAATTCTCCACCTCAATCTGATTTAAGGAGATTCCCTGCTTCAGTGCGACTGGTGACTTGTACACCACTGTTTCCTCTTTCTCATTGGTCAAGGTGACCGTGCGATTCGCACAATCAATGACACCCTTGAACTTAGTCAGCCAATCCAT
1.60 SAM file : RNRRRGRC========N=AA====MCM=V=VRVGSAVGSWVSSRCRMCGMCRMCM=VRSBSMSKSAVMVMSWSSSWSRSGCRMCGMCRMCM=SRVTV=VCSRVMVMSRSGMHSVSRSBSR========S=W=====V=HC=A==D=GD=A==========R=BA====D=CK====CYGAGMCYGMRCCYGVGDCYGAGMCYGMGDCYGVMDCYGARSCYGARCCYGARMCYGVGCCYGMRMCYGAGDCYGMRMCYGVGHCYGARMMYMACYRGRSCYRGGDCYGARSCYGMGDCYGVRMCYRGRVCYGVRMCYRGRMCYRGRMCYGMRVCYRGRMCYRGRC
1.58 GAF file : cs:Z:*CT*TT*CT*TC*GT*CT*TG*AA*GC:1*TT*CC*GT*TG*TC*GT*GA*CC*TT*GG*CC*CC*AA*TT*TT*CC*TT:1*TT*AA*CC*GG*GG*CC:1*TT*CC*TT*TT*GG*TT*AA*AA*AA*TT*TT:1*GG:1*GG*CC*GG*GG*TT*TT*CC:1*TT*GG*GG*TT*GG:1*CC*AA*AA*AA*TT*CC*AA*AA*TT:1*CC*GG*GG*AA*AA*TT*TT*CC*AA*AA*TT:1*TT*CC*TT*CC*TT*CC*TT*TT*GG*GG*GG*TT*GG*GG*CC*AA*TT*AA*GG*TT*AA*GG*TT*GG*AA*GG*GG*TT*CC:1*TT*CC*AA*GG*GG*GG*AA*AA*CC*AA*CC*CC*TT*CC*TT*GG*GG*AA*TT*AA*CC*TT*CC*GG*CC*AA*GG*AA*CC*TT*AA*TT*GG*GG*GG*GG*AA*TT*AA*TT*CC*TT*TT*CC*CC*AA*AA*TT:1*TT*CC*CC*TT*CC*CC*AA*AA*CC*TT*CC*TT*TT*CC*TT*CC*CC*TT*CC*GG*GG*TT*GG*AA*CC*CC*AA*CC*CC*GG*AA*AA*TT*TT*CC*TT*CC*CC*AA*CC*CC*TT*CC*AA*AA*TT*CC*TT*GG*AA*TT*TT*TT*AA*AA*GG*GG*AA*GG*AA*TT*TT:1*CC*CC*TT*GG*CC*TT*TT*CC:1*GG*TT:1*CC:3*TT:1*GG*TT:1*AA*CC*TT*TT:1*TT*AA*CC*AA*CC*CC:2*TT*GG*TT*TT*TT:1*CC*TT*CC*TT*TT*TT*CC*TT*CC*AA*TT*TT*GG*GG*TT:1*AA*AA*GG:1*TT*GG*AA*CC*CC:1*TT*GG*CC*GG*AA*TT*TT:1*GG*CC*AA*CC*AA*AA*TT*CC*AA*AA*TT*GG*AA*CC*AA*CC*CC:1*TT*TT*GG*AA:2*TT*TT*AA:1*TT:1*AA:1*CC*CC:1*AA*TT*CC*CC*AA*TT
1.60 GAF file : cs:Z:*CR*TN*CR*TR*GR*CG*TR*AC*G=*C=*T=*C=*G=*T=*T=*G=*GN*C=*TA*GA*C=*C=*A=*T=*TM:1*TM*G=*TV*A=*CV*GR*GV*CG*CS*TA*CV*TG*TS*GW*TV*AS*AS*AR*TC*TR*GM*GC:1*GM:1*GR*GM*TC*TM*C=*CV*TR*GS*GB*TS*GM*CS*CK*AS:1*AV*TM*CV*AM*AS*TW*CS*CS*GS*GW*AS*AR*TS*TG:1*AR*AM*TC*CG*TM:1*TR*CM*TC*CM*T=*TS*GR*GV*GT*TV*G=*GV:1*AS*TR*AV*GM*TV*AM*GS*TR*GS*AG*GM*GH*TS*CV*CS*TR*CS*AB*GS*GR*G=*A=*A=*C=*A=*C=*C=*T=*CS*T=*GW*G=*A=*T=*A=*C=*TV*C=*GH:1*A=*GA*A=*C=*TD*A=*TG*GD*G=*GA*G=*A=*T=*A=*T=*C=*T=*T=*C=*C=*AR*A=*TB*TA*T=*C=*C=*T=*CD*C=*AC*AK*C=*T=*C=*T=*TC*CY*TG*CA*CG*TM:1*GY:1*TM*GR*AC:1*CY*AG*CV*CG*GD*AC*AY*TG*TA*CG*TM:1*CY*AG*CM*CG*TD:1*AY*AG*TV*CM*TD*GC*AY*TG*TA*TR*AS*AC*GY:2*GR*AC*TC*TY*CG*CA*CR*TM*GC*CY*TG*TV*CG*AC*GC*TY:1*CM*GR*AM:1*TY:1*GA*TG*GD*AC*CY*TG*TM*GR*TM*AC*CY*AG*CV*CG*AH:1*TY:1*TA*TR*TM*CM*CY*TM*CA*TC*TY*TR*CG*TR*CS*AC*TY*TR:2*TD:1*AY*AG*GA*GR*TS*GC*AY*CG*CM:1*TD*GC*CY:1*AV*TR*TM:1*GY*CR*AG*CR*AV*AC*TY*CG*AV*AR*TM*GC*AY*CR*AG*CR*CM:1*TY*TR:1*AR*AM:1*TY*TG*AM*GR*TV:1*AY*GR*CG*CR*AM*AC*TY*CR*CG*AR*TC
vg version v1.60.0 "Annicco"
Compiled with g++ (Ubuntu 9.4.0-1ubuntu1~20.04.2) 9.4.0 on Linux
Linked against libstd++ 20210601
Built by root@buildkitsandbox
or
vg version v1.58.0 "Cartari"
Compiled with g++ (Ubuntu 9.4.0-1ubuntu1~20.04.2) 9.4.0 on Linux
Linked against libstd++ 20210601
Built by root@buildkitsandbox
Thank you for your help.
The text was updated successfully, but these errors were encountered:
1. What were you trying to do?
I was trying to report coordinates from a linear genome onto the graph using
vg inject
, and report the coordinates from the graph to a target linear genome usingvg surject
.I converted a BED file with my coordinates to a BAM file using
bedtools bedtobam
. Then I used the BAM file withvg inject
to output a GAF file. The GAF file was used byvg surject
to output a SAM file.2. What did you want to happen?
I wanted the resulting SAM file to have the sequence corresponding to my coordinates in the target genome.
3. What actually happened?
When I used vg version 1.58 it worked as expected, but with vg version 1.59 and 1.60, the sequence field in the SAM file has a surprising sequence that doesn't correspond to DNA, and I don't what it corresponds to. The strange sequence in version 1.59 and 1.60 has the same length as the DNA sequence in version 1.58.
When I compared the GAF files from
vg inject
, it appears the problems comes from these files, sovg inject
seems responsible.Here is an example of the strange sequence in the SAM (field 10) and GAF (field 12) files :
1.58 SAM file :
TTTCTTGACCTCTGCTACTGCCATTCTGTACGGCCTCTTGTAAATTGGGGCGGTTCCTGGTGCCAAATCAATCCGGAATTCAATCTCTCTCTTGGGTGGCATAGTAGTGAGGTCCTCAGGGAACACCTCTGGATACTCGCAGACTATGGGGATATCTTCCAATTTCCTCCAACTCTTCTCCTCGGTGACCACCGAATTCTCCACCTCAATCTGATTTAAGGAGATTCCCTGCTTCAGTGCGACTGGTGACTTGTACACCACTGTTTCCTCTTTCTCATTGGTCAAGGTGACCGTGCGATTCGCACAATCAATGACACCCTTGAACTTAGTCAGCCAATCCAT
1.60 SAM file :
RNRRRGRC========N=AA====MCM=V=VRVGSAVGSWVSSRCRMCGMCRMCM=VRSBSMSKSAVMVMSWSSSWSRSGCRMCGMCRMCM=SRVTV=VCSRVMVMSRSGMHSVSRSBSR========S=W=====V=HC=A==D=GD=A==========R=BA====D=CK====CYGAGMCYGMRCCYGVGDCYGAGMCYGMGDCYGVMDCYGARSCYGARCCYGARMCYGVGCCYGMRMCYGAGDCYGMRMCYGVGHCYGARMMYMACYRGRSCYRGGDCYGARSCYGMGDCYGVRMCYRGRVCYGVRMCYRGRMCYRGRMCYGMRVCYRGRMCYRGRC
1.58 GAF file :
cs:Z:*CT*TT*CT*TC*GT*CT*TG*AA*GC:1*TT*CC*GT*TG*TC*GT*GA*CC*TT*GG*CC*CC*AA*TT*TT*CC*TT:1*TT*AA*CC*GG*GG*CC:1*TT*CC*TT*TT*GG*TT*AA*AA*AA*TT*TT:1*GG:1*GG*CC*GG*GG*TT*TT*CC:1*TT*GG*GG*TT*GG:1*CC*AA*AA*AA*TT*CC*AA*AA*TT:1*CC*GG*GG*AA*AA*TT*TT*CC*AA*AA*TT:1*TT*CC*TT*CC*TT*CC*TT*TT*GG*GG*GG*TT*GG*GG*CC*AA*TT*AA*GG*TT*AA*GG*TT*GG*AA*GG*GG*TT*CC:1*TT*CC*AA*GG*GG*GG*AA*AA*CC*AA*CC*CC*TT*CC*TT*GG*GG*AA*TT*AA*CC*TT*CC*GG*CC*AA*GG*AA*CC*TT*AA*TT*GG*GG*GG*GG*AA*TT*AA*TT*CC*TT*TT*CC*CC*AA*AA*TT:1*TT*CC*CC*TT*CC*CC*AA*AA*CC*TT*CC*TT*TT*CC*TT*CC*CC*TT*CC*GG*GG*TT*GG*AA*CC*CC*AA*CC*CC*GG*AA*AA*TT*TT*CC*TT*CC*CC*AA*CC*CC*TT*CC*AA*AA*TT*CC*TT*GG*AA*TT*TT*TT*AA*AA*GG*GG*AA*GG*AA*TT*TT:1*CC*CC*TT*GG*CC*TT*TT*CC:1*GG*TT:1*CC:3*TT:1*GG*TT:1*AA*CC*TT*TT:1*TT*AA*CC*AA*CC*CC:2*TT*GG*TT*TT*TT:1*CC*TT*CC*TT*TT*TT*CC*TT*CC*AA*TT*TT*GG*GG*TT:1*AA*AA*GG:1*TT*GG*AA*CC*CC:1*TT*GG*CC*GG*AA*TT*TT:1*GG*CC*AA*CC*AA*AA*TT*CC*AA*AA*TT*GG*AA*CC*AA*CC*CC:1*TT*TT*GG*AA:2*TT*TT*AA:1*TT:1*AA:1*CC*CC:1*AA*TT*CC*CC*AA*TT
1.60 GAF file :
cs:Z:*CR*TN*CR*TR*GR*CG*TR*AC*G=*C=*T=*C=*G=*T=*T=*G=*GN*C=*TA*GA*C=*C=*A=*T=*TM:1*TM*G=*TV*A=*CV*GR*GV*CG*CS*TA*CV*TG*TS*GW*TV*AS*AS*AR*TC*TR*GM*GC:1*GM:1*GR*GM*TC*TM*C=*CV*TR*GS*GB*TS*GM*CS*CK*AS:1*AV*TM*CV*AM*AS*TW*CS*CS*GS*GW*AS*AR*TS*TG:1*AR*AM*TC*CG*TM:1*TR*CM*TC*CM*T=*TS*GR*GV*GT*TV*G=*GV:1*AS*TR*AV*GM*TV*AM*GS*TR*GS*AG*GM*GH*TS*CV*CS*TR*CS*AB*GS*GR*G=*A=*A=*C=*A=*C=*C=*T=*CS*T=*GW*G=*A=*T=*A=*C=*TV*C=*GH:1*A=*GA*A=*C=*TD*A=*TG*GD*G=*GA*G=*A=*T=*A=*T=*C=*T=*T=*C=*C=*AR*A=*TB*TA*T=*C=*C=*T=*CD*C=*AC*AK*C=*T=*C=*T=*TC*CY*TG*CA*CG*TM:1*GY:1*TM*GR*AC:1*CY*AG*CV*CG*GD*AC*AY*TG*TA*CG*TM:1*CY*AG*CM*CG*TD:1*AY*AG*TV*CM*TD*GC*AY*TG*TA*TR*AS*AC*GY:2*GR*AC*TC*TY*CG*CA*CR*TM*GC*CY*TG*TV*CG*AC*GC*TY:1*CM*GR*AM:1*TY:1*GA*TG*GD*AC*CY*TG*TM*GR*TM*AC*CY*AG*CV*CG*AH:1*TY:1*TA*TR*TM*CM*CY*TM*CA*TC*TY*TR*CG*TR*CS*AC*TY*TR:2*TD:1*AY*AG*GA*GR*TS*GC*AY*CG*CM:1*TD*GC*CY:1*AV*TR*TM:1*GY*CR*AG*CR*AV*AC*TY*CG*AV*AR*TM*GC*AY*CR*AG*CR*CM:1*TY*TR:1*AR*AM:1*TY*TG*AM*GR*TV:1*AY*GR*CG*CR*AM*AC*TY*CR*CG*AR*TC
5. What data and command can the vg dev team use to make the problem happen?
The data is available here : https://filesender.renater.fr/?s=download&token=a1a584c0-46e3-4ff9-8eef-20c916237644
6. What does running
vg version
say?or
Thank you for your help.
The text was updated successfully, but these errors were encountered: