- Geno imputation
- Pipeline
- Setup and software dependencies
- Common folder tree
- Split collection files.
- Automatically summarize raw data in folder tree.
- Prepare marker map files.
- Convert raw-data to Plink text input files
- Convert Plink text input files to binary files
- Merge converted plink files
- QC of converted raw data before imputation.
- Checklist: Validate that Tim's tacit knowledge is accounted for!
- Convert to alphaimpute format
- Documentation and scripts for imputation.
Created by gh-md-toc
Welcome to the Geno Imputation github repository. We want this to b a common code base for developing the genotype reference for Geno imputed with AlphaImpute. Our goal is to write scripts that both document and does the convertion from Chip raw-data and imputation. As long as the software dependencies are met and the setup code block is edited we should be able to run the scripts on the HPC clusters in Ås / Edinburg / Oslo and on Geno's production server.
Link to other .md docs,, or have everything here with a big TOC at the top.
First set up some user-specific variables pointing to a clone of this repository, the snptranslate repository, raw genotype data from the ftp server ftpgeno.geno.no. The code below dependends on the Bash version 4, Python 2 with numpy (for snptranslate) and R with a few packages (data.table,knitr).
#user-specific variable
prefix=/mnt/users/gjuvslan/geno/geno_imputation #git clone of https://github.com/argju/geno_imputation
ftpgeno=/mnt/users/gjuvslan/geno/geno_imputation/ftpgeno #raw genotype data from ftpgeno.geno.no:/avlgeno/Raw_Data_Files
snptranslatepath=/mnt/users/gjuvslan/geno/snptranslate/ #git clone of https://github.com/timknut/snptranslate.git
export PATH=$PATH:$snptranslatepath
#check required software
set -e
echo "#### Checking dependency Python 2 with Numpy."
python -c "import sys; assert sys.version_info[0]==2; import numpy as np"
echo "#### Checking dependency Plink 1.9."
plink --version | grep v1.9
echo "#### Checking dependency R with packages data.table, knitr, ggplot2, DT."
R -e 'library(data.table); library(knitr); library(ggplot2); library(DT)'
set +e
#check raw genotype data
Nrawfiles=$(ls -1 $ftpgeno/Raw_Data_Files/FinalReport* $ftpgeno/Raw_Data_Files/*.calls.txt | wc -l)
if [ "$Nrawfiles" -ge "90" ]
then
echo "Found all unzipped raw genotype files in $ftpgeno"
else
echo "Did not find all raw genotype files in $ftpgeno."
ls -1 $ftpgeno/Raw_Data_Files/FinalReport*.txt $ftpgeno/Raw_Data_Files/Batch*.txt
echo "Run scripts/download_raw_data to get all the raw data."
exit 1
fi# Code to from the raw data from ftpgeno.geno.no:/avlgeno/Raw_Data_Files to the common folder tree
cd genotype_rawdata
mkdir -p affymetrix25k illumina54k_v1 illumina54k_v2 illumina54k_v2/collections illumina777k affymetrix54k
ln -s -T $ftpgeno/Dropbox/affy25k/plink_txt/affy25K_flipped2_affy50K.txt affymetrix25k/FinalReport_25k.txt
ln -s -t marker_mapfiles $ftpgeno/Dropbox/affy25k/plink_ped/affy25K_flipped2_affy50K.map
ln -s -t illumina54k_v1 $ftpgeno/Raw_Data_Files/FinalReport_54kV1*
ln -s -t illumina54k_v2 $ftpgeno/Raw_Data_Files/FinalReport_54kV2*
ln -s -t illumina54k_v2/ $ftpgeno/Raw_Data_Files/Nordic_54k*
ln -s -t illumina54k_v1/ $ftpgeno/Raw_Data_Files/Swedish_54k_ed1.txt
mv illumina54k_v2/FinalReport_54kV2_collection* illumina54k_v2/collections
ln -s -t illumina777k $ftpgeno/Raw_Data_Files/FinalReport_777k*
ln -s -t illumina777k/ $ftpgeno/Raw_Data_Files/Nordic_HDexchange_201110.txt
ln -s -t affymetrix54k/ $ftpgeno/Raw_Data_Files/*.calls.txt
cd ..The bash code below extracts key information from the raw data files, and this is collected into a full report in reports/genotypereport.Rmd .
cd genotype_rawdata
#grep Illumina FinalReports for headers, normalize whitespace and create table
grep -A 8 -m 1 "\[Header" illumina*/*/Final* illumina*/Final* | grep -v -e "\[" -e "--" > tmp
sed -i -e s/Processing\\t/Processing" "/g -e s/Num\\t/Num" "/g -e s/Total\\t/Total" "/g tmp
sed -i -e s/GSGT\\t/GSGT" "/g -e s/2010\\t/2010" "/g -e s/54\\t/54" "/g tmp
sed -i -e s/bovinehd-manifest-b.bpm/bovinehd_manifest_b.bpm/g -e 's/\r$//' tmp
cat tmp | sed -e s/-/\\t/g -e s/\\t\\t/\\t/g > illumina_headers
## grep Illumina FinalReports for ids, normalize whitespace and create table of filenames and ids
# 1. files in GenomeMatrix format
matrixfiles=illumina54k_v2/collections/Final*" "illumina777k/FinalReport_777k_apr2015.txt" "illumina777k/FinalReport_777k_jun2015.txt" "illumina54k_v2/FinalReport_54kV2_nov2011_ed1.txt" "illumina777k/FinalReport_777k.txt
grep -A 1 -m 1 "\[Data" $matrixfiles | grep -v -e "\[Data" -e "--" > tmp
sed -i -e s/FinalReport_777k.txt-2402/FinalReport_777k.txt-\\t2402/g tmp #FinalReport_777k.txt lacks tab before first ID
cat tmp | sed -e s/-//g -e s/[[:space:]]/\\t/g | awk '{for(i=2;i<=NF;i++) print $1"\t"$i}' > illumina_ids
rm illumina_formats
for file in $matrixfiles; do echo -e $file"\t"Genomematrix >> illumina_formats ; done
# 2. files in GenomeList format (~10 min)
listfiles=illumina54k_v1/Final*" "illumina54k_v2/FinalReport_54kV2_feb2011_ed1.txt" "illumina54k_v2/FinalReport_54kV2_genoskan.txt" "illumina54k_v2/FinalReport_54kV2_ed1.txt" "illumina777k/FinalReport_777k_jan2015.txt" "illumina54k_v1/Swedish_54k_ed1.txt" "illumina54k_v2/Nordic*.txt" "illumina777k/Nordic_HDexchange_201110.txt" "affymetrix25k/FinalReport_25k.txt
time for file in $listfiles; do grep -q $file illumina_ids_list && echo 'Skipping (already in illumina_ids_list)' $file || ./id.R $file >> illumina_ids_list;done
cat illumina_ids_list >> illumina_ids
for file in $listfiles; do echo -e $file"\t"Genomelist >> illumina_formats ; done
## Affymetrix reports
grep -E "time-str|chip-type|cel-count" affymetrix54k/*.calls.txt > tmp
sed -i -e s/:#%/\\t/g -e s/affymetrix-algorithm-param-apt-//g -e s/=/\\t/ tmp
sed -e s/time-str/Date/g -e s/opt-chip-type/Chip/g -e s/opt-cel-count/Nsamples/g tmp > affymetrix_headers
for file in $(ls affymetrix54k/*.calls.txt)
do
if [ $(grep -q $file affymetrix_headers)==0 ]
then
echo "Skipping SNP count for "$file", already in affymetrix_headers"
else
echo "Counting SNPs in $file"
echo -n -e "$file\tNum SNPs\t$(grep AX-[[:digit:]]* $file | cut -f 1 | wc -l)\n" >> affymetrix_headers
fi
done
grep probeset affymetrix54k/*.calls.txt | awk '{for (i=2; i<=NF; i++) print $1"\t"$i}' > tmp
sed -e s/:probeset_id//g tmp > affymetrix_ids
cd ..ioSNP.py will create the plink map file, which is a better solution than doing it manually in R.
Annotation files for 50Kv1&v2 and 777K Illumina chips was downloaded from SNPchimp and the tab-separated gzipped raw files are in genotype_rawdata/marker_mapfiles/snpchimp/. The SNP positions refer to a particular genom assembly and choosing "Native platform" will not give consistent SNP positions across chips, we should therefore use the "UMD3.1" positions when creating plink files, see snpchimp.Rmd / snpchimp.pdf for details on the SNPchimp data.
cd genotype_rawdata/marker_mapfiles/
#marker map files for Illumina chips with Native platform positions
zgrep Bov_Illu50Kv1 snpchimp/illumina_50Kv1_50Kv2_777K_native.tsv.gz | gawk '{print $5"\t"$7"\t"0"\t"$6}' > illumina50Kv1_annotationfile_native.map
zgrep Bov_Illu50Kv2 snpchimp/illumina_50Kv1_50Kv2_777K_native.tsv.gz | gawk '{print $5"\t"$7"\t"0"\t"$6}' > illumina50Kv2_annotationfile_native.map
zgrep Bov_IlluHD snpchimp/illumina_50Kv1_50Kv2_777K_native.tsv.gz | gawk '{print $5"\t"$7"\t"0"\t"$6}' > illumina777K_annotationfile_native.map
#marker map files for Illumina chips with UMD3.1 dbSNP positions
zgrep Bov_Illu50Kv1 snpchimp/illumina_50Kv1_50Kv2_777K_UMD3.1.tsv.gz | gawk '{print $5"\t"$7"\t"0"\t"$6}' | sed s/^99/0/ > illumina50Kv1_annotationfile_umd3_1.map
zgrep Bov_Illu50Kv2 snpchimp/illumina_50Kv1_50Kv2_777K_UMD3.1.tsv.gz | gawk '{print $5"\t"$7"\t"0"\t"$6}' | sed s/^99/0/ > illumina50Kv2_annotationfile_umd3_1.map
zgrep Bov_IlluHD snpchimp/illumina_50Kv1_50Kv2_777K_UMD3.1.tsv.gz | gawk '{print $5"\t"$7"\t"0"\t"$6}' | sed s/^99/0/ > illumina777K_annotationfile_umd3_1.map
#marker map file for Affymetrix 50K chip
cut -f 1-4 affy50k_annotation_final_list_20160715.txt | grep -v NA > affymetrix50K.map
cd ../..Convert the Affymetrix and Illumina genotype report files to Plink standard text input files (.ped) files paired up with matching .map files.
Use snptranslate-script from https://github.com/timknut/snptranslate/blob/master/seqreport_edit.py to convert from Affymetrix format to Geno format. Then use ioSNP.py to convert from Geno to Plink text input format.
#Convert Affymetrix 50K files
markermap_affy=genotype_rawdata/marker_mapfiles/affy50k_annotation_merged_20170119.txt
mkdir -p genotype_data/plink_txt genotype_data/plink_bin genotype_data/reports
for affycall in `gawk '{print $1}' genotype_rawdata/affymetrix_headers | sort | uniq`
do
pedfile=genotype_data/plink_txt/$(basename $affycall).ped
if [ -e $pedfile ]
then
echo "Skipping conversion of: "$affycall", plink input file exists: "$pedfile
else
echo -e "Converting Affymetrix report file: "$affycall"\tMarkermap: "$markermap_affy
time seqreport_edit.py -m $markermap_affy -o genotype_data/plink_txt/$(basename $affycall) -r genotype_data/reports/$(basename $affycall).seqreport genotype_rawdata/$affycall
ioSNP.py -i genotype_data/plink_txt/$(basename $affycall) -n Geno -o genotype_data/plink_txt/$(basename $affycall).ped -u Plink --output2 genotype_data/plink_txt/$(basename $affycall).map -m genotype_rawdata/marker_mapfiles/affymetrix50K.map
fi
doneUse ioSNP.py to convert all Finalreport files.
snptranslatepath=/mnt/users/gjuvslan/geno/snptranslate/
export PATH=$PATH:$snptranslatepath
## Assign marker position maps for each chip
declare -A markermap=([illumina54k_v1]=illumina50Kv1_annotationfile_umd3_1.map [illumina54k_v2]=illumina50Kv2_annotationfile_umd3_1.map [illumina777k]=illumina777K_annotationfile_umd3_1.map [affymetrix25k]=affy25K_flipped2_affy50K.map)
## Loop over chips and convert all genotype report files to .ped format
for chip in "${!markermap[@]}"
do
for report in `grep $chip genotype_rawdata/illumina_formats | gawk '{print $1}'`
do
pedfile=genotype_data/plink_txt/$(basename $report).ped
if [ -e $pedfile ]
then
echo "Skipping conversion of: "$report", plink input file exists: "$pedfile
else
echo -e "Converting Illumina report file: "$report"\tMarkermap: "${markermap[$chip]}
informat=$(grep $report[[:space:]] genotype_rawdata/illumina_formats | gawk '{print $2}')
ioSNP.py -i genotype_rawdata/$report -n $informat -o $pedfile".tmp" -u Plink --output2 genotype_data/plink_txt/$(basename $report).map -m genotype_rawdata/marker_mapfiles/${markermap[$chip]} && mv $pedfile".tmp" $pedfile
fi
done
done# convert all .ped and .map files to Plink binary files
for chip in affymetrix25k illumina54k_v1 illumina54k_v2 illumina777k affymetrix54k
do
for file in `grep -h $chip genotype_rawdata/illumina_formats genotype_rawdata/affymetrix_headers | gawk '{print $1}' | sort | uniq`
do
plinkbin=genotype_data/plink_bin/$(basename $file)
if [[ -e $plinkbin".bed" && -e $plinkbin".bim" && -e $plinkbin".fam" ]]
then
echo "Skipping conversion of: "$(basename $file)", plink binaries exist: "$binfile".bim/.bed/.fam"
else
time plink --cow --file genotype_data/plink_txt/$(basename $file) --out genotype_data/plink_bin/$(basename $file)
fi
done
done
#parse plink logs to extract table with number of SNPs and individuals
for file in genotype_data/plink_bin/*.log
do
echo -n $(echo $file | sed s/.log//)
grep single-pass $file | gawk -F "[( ,)]" '{print "\t"$6"\t"$9}'
done- Update IDs and pedigree to GenoId, using mapping file ped_genoid_all.txt (separate repo: geno_imputation_idmapping)
- Write reports on missingness, HW, MAF and heterozygosity
cd genotype_data
## per File id mapping (remove, update ids, update pedigree, update sex)
idmap=$ftpgeno/Id_Raw_Data_Files/ped_genoid_all.txt
mkdir -p plink_bin_updateid plink_bin_updateped updates plink_bin_reports
for chip in affymetrix25k illumina54k_v1 illumina54k_v2 illumina777k affymetrix54k
do
for file in $(grep $chip $idmap | cut -f 2 | uniq)
do
plinkbin=plink_bin_updateped/$(basename $file)
if [[ -e $plinkbin".bed" && -e $plinkbin".bim" && -e $plinkbin".fam" ]]
then
echo "Skipping pedigree update of: "$(basename $file)", plink binaries exist: "$binfile".bim/.bed/.fam"
else
grep "$file.*Remove" $idmap | gawk '{print "F0\t"$3}' | sort |uniq > updates/$file.remove
grep "$file.*Impute" $idmap | gawk -v F="$file" '{print "F0\t"$3"\t"F"\t"$4}' | sort | uniq > updates/$file.ids
plink --cow --bfile plink_bin/$file --remove updates/$file.remove --update-ids updates/$file.ids --make-bed --out plink_bin_updateid/$file
grep "$file.*Impute" $idmap | gawk -v F="$file" '{print F"\t"$4"\t"$5"\t"$6}' | sort | uniq > updates/$file.parents
grep "$file.*Impute" $idmap | gawk -v F="$file" '{print F"\t"$4"\t"$8}' | sort | uniq > updates/$file.sex
plink --cow --bfile plink_bin_updateid/$file --update-parents updates/$file.parents --update-sex updates/$file.sex --make-bed --out plink_bin_updateped/$file
plink --cow --bfile plink_bin_updateped/$file --hardy --missing --het --freqx --nonfounders --out plink_bin_reports/$file
fi
done
done
##check for errors and warnings on id updates
grep -i Error plink_bin_update*/*.log | head
grep -i Warning plink_bin_update*/*.log | head
grep -i Note plink_bin_update*/*.log | head
cd ..See the plink documentation for more information. Given a file that list file prefixes that are to be merged with a plink binary file-set, the following command would work.
cd genotype_data
mkdir -p plink_merged_chip
for chip in affymetrix25k illumina54k_v1 illumina54k_v2 illumina777k affymetrix54k
do
grep $chip ../genotype_rawdata/illumina_formats | cut -f 1 | sed s/$chip/plink_bin_updateped/g | sed s/collections//g > $chip.files
grep $chip ../genotype_rawdata/affymetrix_headers | cut -f 1 | sort | uniq | sed s/$chip/plink_bin_updateped/g >> $chip.files
tail -n +1 $chip.files > $chip.merge
plink --cow --bfile $(head -1 $chip.files) --merge-list $chip.merge --chr 1-29 --alleleACGT --make-bed --out plink_merged_chip/$chip
done- Missingess per animal. 90 %
- Missingness per SNP 90 %
- HWE p < 1e-7
- MAF < 0.01
- Mendelian error filtering per SNP and animal. (Although AlphaImpute do a good job at this.)
- Heterozygosity per animal
Example plink command:
plink --bfile infile --cow --hwe 1e-7 --maf 0.01 --geno 0.90 --mind 0.90
Checklist: Validate that Tim's tacit knowledge is accounted for!
- Flipping: Illumina 50K and 777k should be flipped to the forward strand. She lists in chip-folders on the Dropbox Geno_sharing. Flipping is done by Paolo with
plink --flip - Remap 25k markers on affy 50k Convertion list
final_corrected_Affy25K_on_50K.txtin Dropbox. - Correct subsetting of Affy 50k markers. See list in Affy50k Dropbox folder.
final_list_to_Paolo_may_2016_affy50k_markers2_keep - Markers wrongly positioned because of assembly problems See
assembly_fail_markers_to_exclude_*in affy50k and illumina 777k folders in Dropbox. - Have Paolo recieved and included the latest Affymetrix data?
- Keep most recent or higher density sample when same sample is genotyped on same or several platforms.
See Paolos script. In addition to this, do a chromsomoe loop in plink and do something like for i in seq 1 29; do plink --recode A --chr $i --cow --bfile prefix --out prefix.$i.raw; done
Paolo: TODO