Software for BART-Seq

Software required for BART-Seq – a cost-effective target enrichment technology using forward and reverse barcoding to analyze selected set of genes in single cells and/or bulk RNA/DNA samples

Barcode design

Design barcodes using the bartender python software

Primer design

http://icb-bar.helmholtz-muenchen.de

Design primers using a web app, implemented in python in the bartender software

Demultiplex Sequencing Data

The demultiplexing pipeline lives in the repository bartseq-pipeline

Normalization

Sample R script to normalize read counts for Bart-Seq technology

Authors

• Steffen Sass
• Richa Batra
• Fatma Uzbas
• Nikola Müller
• Philipp Angerer