Systemin perception and signaling rely on the systemin receptor SYR1. However, SYR1-dependent signaling, and how systemin signaling differs from other peptide-mediated signaling pathways, is largely unknown. This experiment contained WT and syr1(systemin receptor knockout) cell culture treated by systemin.Samples were harvested at 0,1,2,5,15,45 min and each time point had 6 biological replicates. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE27 partner repository with the dataset identifier PXD054229.

Script folder contains all analyses(code) done in R; Data folder has results from analyses(scripts folder) as well as tomato genome annotation ITAG3.2; Figure folder has all the plots present in the paper.

PPase linear regression plot (Figure2a) is a linear regression plot showing the phosphate release progress curves of different phosphatases.

Run using R version 4.4.0;Operating system is macOS Monterey(Version 12.7.6).

R packages including limma(3.60.4); ggplot2(3.5.1); tidyverse(2.0.0); missForest(1.5); stringr(1.5.1); factoextra(1.0.7); reshape2(1.4.4); hypeR(2.2.0); ggpubr(0.6.0); viridis(0.6.5).Packages should be compatible with Windows, Mac, and Linux operating systems.

If files in Data folder can not be viewed on-site, please press the [download] button at the upper right corner of the file of interest.