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Gesmira authored and dcollins15 committed Dec 20, 2023
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4 changes: 2 additions & 2 deletions vignettes/run_azimuth_tutorial.Rmd
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This vignette demonstrates how to map scRNA-seq and scATAC-seq data to Azimuth reference datasets directly in R, without the need to upload data into the web application.

Reference-based mapping provides an attractive alternative to unsupervised analysis. When well-curated and annotated references are available, reference-mapping can rapdidly, robustly, and sensitively annotate and interpret query datasets. As part of the [Human Biomolecular Atlas Project](portal.hubmapconsortium.org), we have built integrated references for multiple human tissues, available at [azimuth.hubmapconsortium.org](azimuth.hubmapconsortium.org). Azimuth is a web-tool that maps user-uploaded datasets - starting from an unnormalized expression counts matrix.
Reference-based mapping provides an attractive alternative to unsupervised analysis. When well-curated and annotated references are available, reference-mapping can rapdidly, robustly, and sensitively annotate and interpret query datasets. As part of the [Human Biomolecular Atlas Project](https://portal.hubmapconsortium.org/), we have built integrated references for multiple human tissues, available at [azimuth.hubmapconsortium.org](https://azimuth.hubmapconsortium.org/). Azimuth is a web-tool that maps user-uploaded datasets - starting from an unnormalized expression counts matrix.

In this vignette, we demonstrate the use of a function `RunAzimuth()` which facilitates annotation of single cell datasets.

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We recently released **Azimuth ATAC**, which uses the bridge integration methodology introduced in Hao, et al 2022. and demonstrated in [this vignette](https://satijalab.org/seurat/articles/bridge_integration_vignette.html). A multimodal bridge dataset, measuring both scRNA-seq and scATAC-seq data per cell, is used to transfer annotations from our high quality RNA references to an ATAC query.
The [Azimuth ATAC web application](https://azimuth.hubmapconsortium.org/) increases app efficiency by using a fast requantification method based on overlap to match the peaks in your query ATAC data to that of the multiome. Thus, users only need to upload the peak-cell matrix from their data.
Alternatively, if users would like to requantify peaks from the information stored in their fragment file, the `RunAzimuth()` function in R will run standard bridge integration using both the peak-cell matrix and the fragment file. For Azimuth ATAC, you can download the reference dataset [here]().
Alternatively, if users would like to requantify peaks from the information stored in their fragment file, the `RunAzimuth()` function in R will run standard bridge integration using both the peak-cell matrix and the fragment file. The workflow for scRNA-seq queries is included [below](https://satijalab.github.io/azimuth/articles/run_azimuth_tutorial.html#scatac-seq-queries).

# Installation

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