"Single-cell new RNA sequencing reveals principles of transcription at the resolution of individual bursts".

This repository contains scripts and tabular data related to 4sU-based single-cell RNA-seq for improved inference of transcriptional bursting kinetics, and transcriptome-wide analysis of co-bursting.

We provide the following tabular data: New RNA counts tables (at allelic resolition) and transcriptome-wide inferred kinetic parameters. Both these are found in the folder data_tables of this repository.

For script, there are three catogories, corresponding to the sections of the manuscript:

In the folder data_processing, you can find scripts and instructions on how to go from NASC-Seq2 sequencing output to molecular counts.

In the folder kinetics_lookup, you can find script for generating burst kinetic estimates from molecular counts, and code we used for plots in the manuscript, simulations and format conversions.

In the folder cobursting, you can find script that were used for our cobursting analysis.

Some of the folders contain additional readme files for further information.

Detailed information on how to pre-process data and run these scripts are provided in the data_processing/demo folder.