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104 changes: 104 additions & 0 deletions docs/source/api/glue-functions.rst
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=================
Utility Functions
=================

Glow includes a variety of utility functions for performing basic data manipulation.

Struct transformations
======================

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I think some motivation or example as to why the user might need these functions is needed. interoperability between bioinformatics tools said above is too generic.

Glow's struct transformation functions change the schema of a struct. These transformations integrate with functions
whose parameter structs require a certain schema.

- ``subset_struct``: subset fields from a struct

.. code-block:: py

from pyspark.sql import Row
row_one = Row(Row(str_col='foo', int_col=1, bool_col=True))
row_two = Row(Row(str_col='bar', int_col=2, bool_col=False))
base_df = spark.createDataFrame([row_one, row_two], schema=['base_col'])
base_df.selectExpr("subset_struct(base_col, 'str_col', 'bool_col') as subsetted_col")

- ``add_struct_fields``: append fields to a struct

.. code-block:: py

base_df.selectExpr("add_struct_fields(base_col, 'float_col', 3.14, 'rev_str_col', \
reverse(base_col.str_col)) as added_col")

- ``expand_struct``: explode a struct into columns

.. code-block:: py

base_df.selectExpr("expand_struct(base_col)")


Spark ML transformations
========================

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Same two comments regarding motivation/example of need and list format.

Glow supports transformations between double arrays and Spark ML vectors for integration with machine learning
libraries such as MLlib.

- ``array_to_dense_vector``: transform from an array to a dense vector

.. code-block:: py

array_df = spark.createDataFrame([Row([1.0, 2.0, 3.0]), Row([4.1, 5.1, 6.1])], schema=['array_col'])
array_df.selectExpr('array_to_dense_vector(array_col) as dense_vector_col')

- ``array_to_sparse_vector``: transform from an array to a sparse vector

.. code-block:: py

array_df.selectExpr('array_to_sparse_vector(array_col) as sparse_vector_col')

- ``vector_to_array``: transform from a vector to a double array

.. code-block:: py

from pyspark.ml.linalg import SparseVector
row_one = Row(vector_col=SparseVector(3, [0, 2], [1.0, 3.0]))
row_two = Row(vector_col=SparseVector(3, [1], [1.0]))
vector_df = spark.createDataFrame([row_one, row_two])
vector_df.selectExpr('vector_to_array(vector_col) as array_col')

- ``explode_matrix``: explode a Spark ML matrix such that each row becomes an array of doubles

.. code-block:: py

from pyspark.ml.linalg import DenseMatrix
matrix_df = spark.createDataFrame(Row([DenseMatrix(2, 3, range(6))]), schema=['matrix_col'])
matrix_df.selectExpr('explode_matrix(matrix_col) as array_col')

Variant data transformations
============================

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Same two comments regarding motivation/example of need and list format.

Glow supports numeric transformations on variant data for downstream calculations, such as GWAS.

- ``genotype_states``: create a numeric representation for each sample's genotype data. This calculates the sum of the
calls (or ``-1`` if any calls are missing); the sum is equivalent to the number of alternate alleles for biallelic
variants.

.. code-block:: py

from pyspark.sql.types import *

missing_and_hom_ref = Row([Row(calls=[-1,0]), Row(calls=[0,0])])
het_and_hom_alt = Row([Row(calls=[0,1]), Row(calls=[1,1])])
calls_schema = StructField('calls', ArrayType(IntegerType()))
genotypes_schema = StructField('genotypes_col', ArrayType(StructType([calls_schema])))
genotypes_df = spark.createDataFrame([missing_and_hom_ref, het_and_hom_alt], StructType([genotypes_schema]))
num_alt_alleles_df = genotypes_df.selectExpr('genotype_states(genotypes_col) as num_alt_alleles_col')

- ``hard_calls``: get hard calls from genotype probabilities. These are determined based on the number of alternate
alleles for the variant, whether the probabilities are phased (true for haplotypes and false for genotypes), and a
call threshold (if not provided, this defaults to ``0.9``). If no calls have a probability above the threshold, the
call is set to ``-1``.

.. code-block:: py

unphased_above_threshold = Row(probabilities=[0.0, 0.0, 0.0, 1.0, 0.0, 0.0], num_alts=2, phased=False)
phased_below_threshold = Row(probabilities=[0.1, 0.9, 0.8, 0.2], num_alts=1, phased=True)
uncalled_df = spark.createDataFrame([unphased_above_threshold, phased_below_threshold])
hard_calls_df = uncalled_df.selectExpr('hard_calls(probabilities, num_alts, phased, 0.95) as calls')
1 change: 1 addition & 0 deletions docs/source/index.rst
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Expand Up @@ -10,4 +10,5 @@ Glow is an open-source genomic data analysis tool using `Apache Spark <https://s
api/variant-qc
api/sample-qc
api/pipe-transformer
api/glue-functions
modules