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import datetime | ||
import os | ||
import glob | ||
from slurmkit import SlurmParams, slurm_function, submit_function | ||
from natsort import natsorted | ||
import click | ||
from iohub import open_ome_zarr | ||
from recOrder.cli.settings import ReconstructionSettings | ||
import torch | ||
from recOrder.io import utils | ||
from recOrder.cli.utils import ( | ||
apply_inverse_to_zyx_and_save, | ||
create_empty_hcs_zarr, | ||
) | ||
from recOrder.cli.compute_transfer_function import ( | ||
compute_transfer_function_cli, | ||
) | ||
|
||
from recOrder.cli.apply_inverse_transfer_function import ( | ||
get_reconstruction_output_metadata, | ||
apply_inverse_transfer_function_single_position, | ||
) | ||
from pathlib import Path | ||
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||
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# Reconstruction parameters | ||
config_path = ( | ||
"/home/eduardo.hirata/repos/recOrder/recOrder/tests/cli_tests/phase.yml" | ||
) | ||
transfer_function_path = "/home/eduardo.hirata/repos/recOrder/recOrder/tests/cli_tests/TF_phase3D.zarr" | ||
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||
# io parameters | ||
input_position_dirpaths = "/home/eduardo.hirata/repos/recOrder/recOrder/tests/cli_tests/data_temp/2022_08_04_20x_04NA_BF.zarr/*/*/*" | ||
output_dirpath = "/home/eduardo.hirata/repos/recOrder/recOrder/tests/cli_tests/test_delete_phase.zarr" | ||
# sbatch and resource parameters | ||
cpus_per_task = 16 | ||
mem_per_cpu = "16G" | ||
time = 40 # minutes | ||
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||
# Path handling | ||
input_position_dirpaths = [ | ||
Path(path) for path in natsorted(glob.glob(input_position_dirpaths)) | ||
] | ||
output_dirpath = Path(output_dirpath) | ||
slurm_out_path = output_dirpath.parent / "slurm_output/recon-%j.out" | ||
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||
transfer_function_path = Path(transfer_function_path) | ||
config_path = Path(config_path) | ||
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## First compute-tf | ||
# Handle transfer function path | ||
# Compute transfer function | ||
compute_transfer_function_cli( | ||
input_position_dirpath=input_position_dirpaths[0], | ||
config_filepath=config_path, | ||
output_dirpath=transfer_function_path, | ||
) | ||
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## Second apply-inv-tf | ||
output_metadata = get_reconstruction_output_metadata( | ||
input_position_dirpaths[0], config_path | ||
) | ||
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||
create_empty_hcs_zarr( | ||
store_path=output_dirpath, | ||
position_keys=[p.parts[-3:] for p in input_position_dirpaths], | ||
**output_metadata, | ||
) | ||
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click.echo(f"in: {input_position_dirpaths}, out: {output_dirpath}") | ||
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# prepare slurm parameters | ||
params = SlurmParams( | ||
partition="cpu", | ||
cpus_per_task=cpus_per_task, | ||
mem_per_cpu=mem_per_cpu, | ||
time=datetime.timedelta(minutes=time), | ||
output=slurm_out_path, | ||
) | ||
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# wrap our process_single_position() function with slurmkit | ||
slurm_reconstruct_single_position = slurm_function( | ||
apply_inverse_transfer_function_single_position | ||
) | ||
reconstruct_func = slurm_reconstruct_single_position( | ||
transfer_function_dirpath=transfer_function_path, | ||
config_filepath=config_path, | ||
num_processes=cpus_per_task, | ||
) | ||
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# generate an array of jobs by passing the in_path and out_path to slurm wrapped function | ||
recon_jobs = [ | ||
submit_function( | ||
reconstruct_func, | ||
slurm_params=params, | ||
input_position_dirpath=input_position_dirpath, | ||
output_position_dirpath=output_dirpath | ||
/ Path(*input_position_dirpath.parts[-3:]), | ||
) | ||
for input_position_dirpath in input_position_dirpaths | ||
] |