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Update minIon_workflow.md
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lerch-a authored Nov 18, 2024
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11 changes: 4 additions & 7 deletions docs/documentation/minIon_workflow.md
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Expand Up @@ -57,7 +57,10 @@ file.copy(from=system.file(package="HaplotypR", "extdata/ex3"), to=".", recursiv
# List files example files in output direcoty
dir(file.path("ex3"))
```
The following files should be listed with the last R command: "marker_file.txt", "reads2_F.fastq.gz", "sample_file.txt".
The following files should be listed with the last R command: "marker_file.txt", "reads3.fastq.gz", "sample_file.txt".


WORK IN PROGRESS

Run demultiplexing by sample and rename output files
```R
Expand Down Expand Up @@ -92,12 +95,6 @@ dePlexMarker <- demultiplexByMarker(dePlexSample, markerTab, outDeplexMarker)
write.table(dePlexMarker, file.path(outputDir, "demultiplexMarkerSummary.txt"), sep="\t", row.names=F)
```

subset: remove markers without sufficient reads
```R
procReads <- procReads[procReads$numRead>10,]

```

Call Haplotypes
```R
# call haplotype options
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