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Schema docs and changelog for --protocol custom
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See also nf-core#41 and nf-core#42
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ewels committed Nov 7, 2020
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1 change: 1 addition & 0 deletions CHANGELOG.md
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Expand Up @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
* Move all parameter documentation into new `nextflow_schema.json` file.
* Update with the latest `TEMPLATE` version for nf-core `v1.11`
* Update conda environment with new packages and updates
* Added `--protocol custom` to allow custom adapter trimming modes [[#41]](https://github.com/nf-core/smrnaseq/issues/41)]

### Packaged software updates

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4 changes: 2 additions & 2 deletions nextflow_schema.json
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"default": "illumina",
"fa_icon": "fas fa-vial",
"description": "Protocol for constructing smRNA-seq libraries.",
"help_text": "Presets for trimming parameters and 3' adapter sequence with a specified protocol.\n\n| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence |\n| :------------ | :-------------------------------------- | :-------------------------------------- | :------------------- |\n| illumina | Illumina TruSeq Small RNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `TGGAATTCTCGGGTGCCAAGG` |\n| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | `clip_r1 = 4` `three_prime_clip_r1 = 4` | `TGGAATTCTCGGGTGCCAAGG` |\n| qiaseq | QIAGEN QIAseq miRNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `AACTGTAGGCACCATCAAT` |\n| cats | Diagenode CATS Small RNA-seq | `clip_r1 = 3` `three_prime_clip_r1 = 0` | `AAAAAAAAAAA` + `GATCGGAAGAGCACACGTCTG` (only polyA is used for trimming) |",
"help_text": "Presets for trimming parameters and 3' adapter sequence with a specified protocol.\n\n| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence |\n| :------------ | :-------------------------------------- | :-------------------------------------- | :--------------------- |\n| illumina | Illumina TruSeq Small RNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `TGGAATTCTCGGGTGCCAAGG` |\n| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | `clip_r1 = 4` `three_prime_clip_r1 = 4` | `TGGAATTCTCGGGTGCCAAGG` |\n| qiaseq | QIAGEN QIAseq miRNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `AACTGTAGGCACCATCAAT` |\n| cats | Diagenode CATS Small RNA-seq | `clip_r1 = 3` `three_prime_clip_r1 = 0` | `AAAAAAAAAAA` + `GATCGGAAGAGCACACGTCTG` (only polyA is used for trimming) |\n| custom | user defined | user defined | user defined |\n\n> NB: When running `--protocol custom` the user ***must define the 3' Adapter Sequence***.\n> If trimming parameters aren't provided the pipeline will deafult to `clip_R1 = 0` and `three_prime_clip_R1 = 0` (i.e. no extra clipping).",
"enum": [
"illumina",
"nextflex",
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"$ref": "#/definitions/institutional_config_options"
}
]
}
}

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