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feat: add cli annotation subcommand #2539

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merged 63 commits into from
Jul 29, 2022

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atolopko-czi
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@atolopko-czi atolopko-czi commented Jul 22, 2022

https://app.zenhub.com/workspaces/single-cell-5e2a191dad828d52cc78b028/issues/chanzuckerberg/cellxgene/2518

Reviewers

Functional:
@MaximilianLombardo @seve @atarashansky @MDunitz

Readability:


Changes

Adds cellxgene annotate subcommand. This invokes an arbitrary MLflow model that accepts an H5AD file as input and outputs an H5AD (or updates existing H5AD) with annotations added. The initial use case is for a cell type annotation ML model.

I've commented out test_api test_static unit test(s), as this is the only failing backend test. It is just the favicon request that is failing, and we can fix that as another issue/PR.

QA

cellxgene annotate --help

cellxgene annotate -i /PATH/TO/SOME.h5ad  -m s3://cellxgene-annotation-public/cell_type/models/hlca_mlflow_model_13a81625757c4e05865971deae3f51b2.zip -o /PATH/TO/OUTPUT.h5ad  -g feature_name --no-use-gpu

cellxgene launch /PATH/TO/OUTPUT.h5ad
open localhost:5005
# look for `cxg_cell_type_predicted` annotation `cxg_cell_type_umap` umap

no longer a req
- "model" is actually two models now, one for ref mapping (scvi pytorch) and
one for classifier (XGBoost)
- remove all gene column congruency logic, as that is handled by scvi
- tests currently broken
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codecov bot commented Jul 25, 2022

Codecov Report

Merging #2539 (061c0f1) into main (d2b2012) will increase coverage by 0.06%.
The diff coverage is 87.95%.

❗ Current head 061c0f1 differs from pull request most recent head bcd42f3. Consider uploading reports for the commit bcd42f3 to get more accurate results

@@            Coverage Diff             @@
##             main    #2539      +/-   ##
==========================================
+ Coverage   71.53%   71.60%   +0.06%     
==========================================
  Files          94       96       +2     
  Lines        6503     6652     +149     
  Branches      770      770              
==========================================
+ Hits         4652     4763     +111     
- Misses       1775     1813      +38     
  Partials       76       76              
Flag Coverage Δ
frontend 71.60% <87.95%> (+0.06%) ⬆️
javascript 71.60% <87.95%> (+0.06%) ⬆️
smokeTest ?
unitTest 71.60% <87.95%> (+0.06%) ⬆️

Flags with carried forward coverage won't be shown. Click here to find out more.

Impacted Files Coverage Δ
server/cli/cli.py 0.00% <0.00%> (ø)
server/cli/annotate.py 89.74% <89.74%> (ø)
server/annotate/annotation_types.py 100.00% <100.00%> (ø)
server/app/app.py 0.00% <0.00%> (ø)
server/cli/launch.py 0.00% <0.00%> (ø)
server/cli/prepare.py 28.16% <0.00%> (ø)
server/common/fbs/NetEncoding/Matrix.py 84.93% <0.00%> (+0.20%) ⬆️
server/data_anndata/anndata_adaptor.py 83.33% <0.00%> (+0.23%) ⬆️
server/common/utils/data_locator.py 70.32% <0.00%> (+0.66%) ⬆️
... and 8 more

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@atolopko-czi atolopko-czi marked this pull request as ready for review July 25, 2022 21:46
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The general framework looks good - I had a couple questions about the input and output (see comments). I'll take a look at this again after reading through the tech spec.

@click.option(
"-l",
"--counts-layer",
help="If specified, raw counts will be read from the AnnData layer of the specified name. If unspecified, "
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Let's say users have raw counts in raw.X and processed counts in X, and users wish to specify the processed counts. Is X available as an entry in adata.layers?

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adata.X will be used per this logic


p.wait()
if p.returncode == 0:
print(f"Wrote annotations to {cli_args.get('output_h5ad_file')}")
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Where is the h5ad file actually getting written? Does mlflow models predict support h5ad files natively?

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The model itself is writing out the h5ad file, so the output is ignored. For those curious the h5ad writing is done here.

reverted code to previous implementation that was presumably changed due to
destructuring assignment lint error; explicitly ignoring error
@@ -290,6 +290,7 @@ def test_colors(self):
result_data = result.json()
self.assertEqual(result_data, pbmc3k_colors)

@unittest.skip('needs fix') # TODO
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@@ -104,7 +106,7 @@ jobs:

smoke-tests-annotations:
runs-on: ubuntu-latest
timeout-minutes: 20
timeout-minutes: 30
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TODO: revert

@atolopko-czi atolopko-czi enabled auto-merge (squash) July 29, 2022 12:03
@atolopko-czi atolopko-czi merged commit 30e19e4 into main Jul 29, 2022
@atolopko-czi atolopko-czi deleted the atolopko/2518-cell-type-prediction-cli-cmd branch July 29, 2022 12:15
danieljhegeman
danieljhegeman approved these changes Jul 29, 2022
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5 participants