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Munsky committed Oct 23, 2023
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## Command Line Version

To get started with the Command line Tools, navigate to the directory "CommandLine" and open one of the tutorial scripts "example_XXX.m". Or you caan start creating and solving models as follows.
To get started with the Command line Tools, navigate to the directory "Examples" and open one of the tutorial scripts "example_XXX.m". We recommend getting started using the following examples:

[Examples/example_SSITBasics.m](https://htmlpreview.github.io/?https://github.com/MunskyGroup/SSIT/blob/main/Examples/html/example_SSITBasics.html) This example will get you started on creating and solving SSIT models.

[Examples/example_EscapeTimes.m](https://htmlpreview.github.io/?https://github.com/MunskyGroup/SSIT/blob/main/Examples/html/example_EscapeTimes.html) This example will get you started on using the SSIT to calculate escape time distributions.

[Examples/example_FIMCalculation.m](https://htmlpreview.github.io/?https://github.com/MunskyGroup/SSIT/blob/main/Examples/html/example_FIMCalculation.html) This example demonstrates the use of the SSIT to calculate Sensitivity of the CME solution and the Fisher Information Matrix.

[Examples/example_FittingAndDesigningExperiments.m](https://htmlpreview.github.io/?https://github.com/MunskyGroup/SSIT/blob/main/Examples/html/example_FittingAndDesigningExperiments.html) This example demonstrates the use of the SSIT and Fisher Information Matrix to design optimally informative experiments.

[Examples/example_HybridModel.m](https://htmlpreview.github.io/?https://github.com/MunskyGroup/SSIT/blob/main/Examples/html/example_HybridModel.html) This example demonstrates the use of the SSIT for creating hybrid models where some (upstream) species evolve determistically while other (downstream) species are stochastic.

[Examples/example_ModelReductionTool.m](https://htmlpreview.github.io/?https://github.com/MunskyGroup/SSIT/blob/main/Examples/html/example_ModelReductionTool.html) This example demonstrates the use of the SSIT for applying linear transformations to reduce the size of the CME solution scheme (e.g., eigvenvalue decomposition, principle orthoginal decomposition, quasi-steady state assumptions, or coarse mesh solutions).

[Examples/example_MultiModelTool.m](https://htmlpreview.github.io/?https://github.com/MunskyGroup/SSIT/blob/main/Examples/html/example_MultiModelTool.html) This example demonstrates the use of the SSIT for fitting multiple different models with shared or related pameters, but fit to different data sets.

Or you can start creating and solving models as follows.

Example for generating an FSI model and fitting it to smFISH data for Dusp1 activation following glucocorticoid stimulation:

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>> Model = Model.loadData('../ExampleData/DUSP1_Dex_100nM_Rep1_Rep2.csv',{'rna','RNA_nuc'});
>> Model.tSpan = unique([Model.initialTime,Model.dataSet.times]);
>> fitOptions = optimset('Display','iter','MaxIter',100);
>> pars,likelihood] = Model.maximizeLikelihood([],fitOptions);
>> [pars,likelihood] = Model.maximizeLikelihood([],fitOptions);
Update Model and Make Plots of Results

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