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filterCRs IndexError:list index out of range #88
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For step2, scTE requires the reads has both CB and UB tags. How dose the Pan_troglodytes_C1_scTEtmp/o1 and Pan_troglodytes_C1_scTEtmp/o2 folder looks like, seems the format of *count.gz file under the Pan_troglodytes_C1_scTEtmp/o2 are incorrect. |
What's the *count.gz file looks like? |
I can't see any problem. Could you try using a different file? Maybe this error is specific to this file. Can you run scTE succssufully for the test.bam file under the scTE folder? |
I have a total of four samples, then all of them were quantified using cellranger atac, scTE for TE count, the other three were normal, only this sample data showed the following error |
Yes, there are some hidden problems with this input file, I'm also not aware of what issues there might be |
Hello!
When I run the software according to the following code, there is an index error. How can I solve this problem?
Here's my code:
step1:build index
scTE_build -g panTro6
step2: filter bam
samtools view possorted_genome_bam.bam -h | awk '/^@/ || /CB:/' | samtools view -h -b > possorted_genome_bam_CB.bam
samtools view possorted_genome_bam.bam -h | awk '/^@/ || /UB:/' | samtools view -h -b > possorted_genome_bam_UB.bam
step3: TE count
scTE -i /work/xtt/data_input/fastq_out/Pan_troglodytes_C1_out/outs/possorted_genome_bam_UB.bam
-o Pan_troglodytes_C1
-x /work/xtt/biosoft/scTE_ref/panTro6.exclusive.idx
--hdf5 True -CB CB -UMI UB -p 40
echo "Pan_troglodytes_C1 done!"
The possorted_genome_bam_UB.bam file format is as follows:

The error message is as follows:

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