Immunology: DP -> DN -> SP -> B, T Cells
- Conducted pairwise comparisons in in-vivo data
- Drew mRNA expression correlation map
- Processed STAR-aligned data with DESEQ2 and GSEA
- Counted significant numbers for each step
- Identified Ribosome, Oxidative Phosphorylation, and Cell Cycle as common pathways for all stages
- Utilized Cytoscape for path enrichment network analysis to explore pathway interactions
- Nodes: KEGG Gene Sets
- Edges: Common number of gene sets
- Color-coded nodes based on significant adjusted p-values:
- Green: Immune cells (e.g., Allograft Rejection, IGA Production, Cytokine-Cytokine Receptor Interaction, Jackstat Signaling Pathway)
- Orange: Receptor and Immune Cell Signaling
- Blue: DNA, RNA, Protein Synthesis or Regulation related to Cell Development
