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rfm-targa authored Mar 22, 2024
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**chewBBACA** is a software suite for the creation and evaluation of core genome and whole genome MultiLocus Sequence
Typing (cg/wgMLST) schemas and results. The "BBACA" stands for "BSR-Based Allele Calling Algorithm". BSR stands for
BLAST Score Ratio as proposed by [Rasko DA et al.](http://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-6-2). The "chew" part adds extra coolness to the name and could be thought of as "Comprehensive and Highly Efficient Workflow". chewBBACA allows to define the target loci in a schema based on multiple genomes (e.g. define target loci based on the distinct loci identified in a dataset of high-quality genomes for a species or lineage of interest) and performs allele calling to determine the allelic profiles of bacterial strains, easily scaling to thousands of genomes with modest computational resources. chewBBACA includes functionalities to annotate the schema loci, compute the set of loci that constitute the core genome for a given dataset, and generate interactive reports for schema and allele calling results evaluation to enable intuitive analysis of the results in surveillance and outbreak detection settings or population studies. Pre-defined cg/wgMLST schemas can be downloaded from [Chewie-NS ](https://chewbbaca.online/) or adapted from other cg/wgMLST platforms.
BLAST Score Ratio as proposed by [Rasko DA et al.](http://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-6-2). The "chew" part adds extra coolness to the name and could be thought of as "Comprehensive and Highly Efficient Workflow". chewBBACA allows to define the target loci in a schema based on multiple genomes (e.g. define target loci based on the distinct loci identified in a dataset of high-quality genomes for a species or lineage of interest) and performs allele calling to determine the allelic profiles of bacterial strains, easily scaling to thousands of genomes with modest computational resources. chewBBACA includes functionalities to annotate the schema loci, compute the set of loci that constitute the core genome for a given dataset, and generate interactive reports for schema and allele calling results evaluation to enable an intuitive analysis of the results in surveillance and outbreak detection settings or population studies. Pre-defined cg/wgMLST schemas can be downloaded from [Chewie-NS ](https://chewbbaca.online/) or adapted from other cg/wgMLST platforms.

### Check the [documentation](https://chewbbaca.readthedocs.io/en/latest/index.html) for implementation details and guidance on using chewBBACA.

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- Improved BLAST exception capturing.

- CreateSchema and Allelecall exit if any of the input files include blank spaces in the filename.
- CreateSchema and Allelecall exit if input files include blank spaces in the filename.

- Removed global variable that could lead to issues during multiprocessing.

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