diff --git a/src/ObjCRuntime/Runtime.cs b/src/ObjCRuntime/Runtime.cs
index ddf2240c1bdd..38abcc97c069 100644
--- a/src/ObjCRuntime/Runtime.cs
+++ b/src/ObjCRuntime/Runtime.cs
@@ -621,6 +621,12 @@ static IntPtr PrintAllExceptions (IntPtr exception_gchandle)
 		// For XM it will also register all assemblies loaded in the current appdomain.
 		internal static void RegisterAssemblies ()
 		{
+#if NET
+			if (IsNativeAOT) {
+				return;
+			}
+#endif
+
 #if PROFILE
 			var watch = new Stopwatch ();
 #endif
diff --git a/src/ObjCRuntime/Runtime.mac.cs b/src/ObjCRuntime/Runtime.mac.cs
index 9f092f860e95..caaad538a4f5 100644
--- a/src/ObjCRuntime/Runtime.mac.cs
+++ b/src/ObjCRuntime/Runtime.mac.cs
@@ -194,11 +194,13 @@ unsafe static void InitializePlatform (InitializationOptions* options)
 			FrameworksPath = Path.Combine (basePath, "Frameworks");
 		}
 
+#if !NET
 		[Preserve]
 		static IntPtr GetNullableType (IntPtr type)
 		{
 			return AllocGCHandle (Registrar.GetNullableType ((Type) GetGCHandleTarget (type)!));
 		}
+#endif // !NET
 #endif // !COREBUILD
 	}
 }
diff --git a/tests/monotouch-test/ObjCRuntime/RegistrarTest.cs b/tests/monotouch-test/ObjCRuntime/RegistrarTest.cs
index dd17244c45c2..ae30163d06d2 100644
--- a/tests/monotouch-test/ObjCRuntime/RegistrarTest.cs
+++ b/tests/monotouch-test/ObjCRuntime/RegistrarTest.cs
@@ -2246,7 +2246,8 @@ public void TestCtors ()
 			}
 		}
 
-#if __MACOS__
+		// This test uses Assembly.LoadFrom, which isn't supported with NativeAOT
+#if __MACOS__ && !NATIVEAOT
 		[Test]
 		public void CustomUserTypeWithDynamicallyLoadedAssembly ()
 		{
diff --git a/tests/mtouch/Cache.cs b/tests/mtouch/Cache.cs
index c44910246f15..5e93b130ed48 100644
--- a/tests/mtouch/Cache.cs
+++ b/tests/mtouch/Cache.cs
@@ -18,7 +18,11 @@ public static class Cache // Not really a cache (since the root directory is cle
 
 		static Cache ()
 		{
+#if NATIVEAOT
+			root = Path.Combine (Path.GetDirectoryName (Environment.ProcessPath)!, "tmp-test-dir");
+#else
 			root = Path.Combine (Path.GetDirectoryName (System.Reflection.Assembly.GetExecutingAssembly ().Location)!, "tmp-test-dir");
+#endif
 			if (Directory.Exists (root)) {
 				var movedRoot = root + DateTime.UtcNow.Ticks.ToString () + "-deletion-in-progress";
 				// The temporary directory can be big, and it can take a while to clean it out.
diff --git a/tests/xharness/Jenkins/TestVariationsFactory.cs b/tests/xharness/Jenkins/TestVariationsFactory.cs
index 9954bb966ae2..636ffdacd748 100644
--- a/tests/xharness/Jenkins/TestVariationsFactory.cs
+++ b/tests/xharness/Jenkins/TestVariationsFactory.cs
@@ -169,9 +169,8 @@ IEnumerable<TestData> GetTestData (RunTestTask test)
 						if (test.Platform != TestPlatform.MacCatalyst) {
 							yield return new TestData { Variation = "Debug (static registrar)", Registrar = "static", Debug = true, Ignored = !jenkins.TestSelection.IsEnabled (TestLabel.Monotouch) || !jenkins.TestSelection.IsEnabled (PlatformLabel.Mac), };
 							yield return new TestData { Variation = "Debug (static registrar, ARM64)", Registrar = "static", Debug = true, Profiling = false, Ignored = !jenkins.TestSelection.IsEnabled (TestLabel.Monotouch) || !jenkins.TestSelection.IsEnabled (PlatformLabel.Mac) || !mac_supports_arm64, RuntimeIdentifier = arm64_runtime_identifier, };
-							// Pending: We need the NativeAOT's runtime bits to ship using runtime packs for macOS (https://github.com/dotnet/runtime/issues/87060) before we can enable this
-							// yield return new TestData { Variation = "Release (NativeAOT)", Debug = false, PublishAot = true, Ignored = !jenkins.TestSelection.IsEnabled (TestLabel.Monotouch) || !jenkins.TestSelection.IsEnabled (PlatformLabel.Mac), Defines = "NATIVEAOT", LinkMode = "Full" };
-							// yield return new TestData { Variation = "Release (NativeAOT, ARM64)", Debug = false, PublishAot = true, Ignored = !jenkins.TestSelection.IsEnabled (TestLabel.Monotouch) || !jenkins.TestSelection.IsEnabled (PlatformLabel.Mac) || !mac_supports_arm64, Defines = "NATIVEAOT", RuntimeIdentifier = arm64_runtime_identifier, LinkMode = "Full" };
+							yield return new TestData { Variation = "Release (NativeAOT, ARM64)", Debug = false, PublishAot = true, Ignored = !jenkins.TestSelection.IsEnabled (TestLabel.Monotouch) || !jenkins.TestSelection.IsEnabled (PlatformLabel.Mac) || !mac_supports_arm64, Defines = "NATIVEAOT", RuntimeIdentifier = arm64_runtime_identifier, LinkMode = "Full" };
+							yield return new TestData { Variation = "Release (NativeAOT, x64)", Debug = false, PublishAot = true, Ignored = !jenkins.TestSelection.IsEnabled (TestLabel.Monotouch) || !jenkins.TestSelection.IsEnabled (PlatformLabel.Mac), Defines = "NATIVEAOT", RuntimeIdentifier = "osx-x64", LinkMode = "Full" };
 						}
 						if (test.Platform == TestPlatform.MacCatalyst) {
 							yield return new TestData { Variation = "Release (ARM64, LLVM)", Debug = false, UseLlvm = true, Ignored = !jenkins.TestSelection.IsEnabled (TestLabel.Monotouch) || !jenkins.TestSelection.IsEnabled (PlatformLabel.MacCatalyst) || !mac_supports_arm64, RuntimeIdentifier = arm64_runtime_identifier };
diff --git a/tools/common/Optimizations.cs b/tools/common/Optimizations.cs
index 16e40bdf4c4d..b6ea74fdc497 100644
--- a/tools/common/Optimizations.cs
+++ b/tools/common/Optimizations.cs
@@ -188,7 +188,7 @@ public void Initialize (Application app, out List<ProductException> messages)
 				// This means it won't be listed in --help, and it won't be enabled if all optimizations
 				// are enabled. Yet we still might want to enable it manually, and this condition
 				// allows us to manually pass --optimize=remove-dynamic-registrar and enable it that way.
-				if (app.Platform == ApplePlatform.MacOSX && (Opt) i == Opt.RemoveDynamicRegistrar)
+				if (app.Platform == ApplePlatform.MacOSX && app.XamarinRuntime != XamarinRuntime.NativeAOT && (Opt) i == Opt.RemoveDynamicRegistrar)
 					continue;
 
 				// check if the optimization is valid for the current platform
@@ -264,7 +264,7 @@ public void Initialize (Application app, out List<ProductException> messages)
 
 			// We will register protocols if the static registrar is enabled and loading assemblies is not possible
 			if (!RegisterProtocols.HasValue) {
-				if (app.Platform != ApplePlatform.MacOSX) {
+				if (app.Platform != ApplePlatform.MacOSX || app.XamarinRuntime == XamarinRuntime.NativeAOT) {
 					RegisterProtocols = (app.Registrar == RegistrarMode.Static || app.Registrar == RegistrarMode.ManagedStatic) && !app.UseInterpreter;
 				} else {
 					RegisterProtocols = false;
diff --git a/tools/common/StaticRegistrar.cs b/tools/common/StaticRegistrar.cs
index 1977fec031b9..b907dedd6bdf 100644
--- a/tools/common/StaticRegistrar.cs
+++ b/tools/common/StaticRegistrar.cs
@@ -3424,6 +3424,12 @@ bool SpecializeTrampoline (AutoIndentStringBuilder sb, ObjCMethod method, List<E
 				sb.AppendLine ("}");
 				return true;
 			case Trampoline.CopyWithZone2:
+#if NET
+				// Managed Static Registrar handles CopyWithZone2 in GenerateCallToUnmanagedCallersOnlyMethod
+				if (LinkContext.App.Registrar == RegistrarMode.ManagedStatic) {
+					return false;
+				}
+#endif
 				sb.AppendLine ("-(id) copyWithZone: (NSZone *) zone");
 				sb.AppendLine ("{");
 				sb.AppendLine ("return xamarin_copyWithZone_trampoline2 (self, _cmd, zone);");
@@ -3472,6 +3478,7 @@ bool TryGetReturnType (ObjCMethod method, string descriptiveMethodName, List<Exc
 			case Trampoline.X86_DoubleABI_StretTrampoline:
 			case Trampoline.StaticStret:
 			case Trampoline.Stret:
+			case Trampoline.CopyWithZone2:
 				switch (method.NativeReturnType.FullName) {
 				case "System.Int64":
 					rettype = "long long";
@@ -4351,6 +4358,14 @@ void GenerateCallToUnmanagedCallersOnlyMethod (AutoIndentStringBuilder sb, ObjCM
 				sb.WriteLine ($"bool call_super = false;");
 			if (hasReturnType)
 				sb.WriteLine ($"{callbackReturnType} rv = {{ 0 }};");
+			if (method.CurrentTrampoline == Trampoline.CopyWithZone2) {
+				sb.WriteLine ("id p0 = (id)zone;");
+				sb.WriteLine ("GCHandle gchandle;");
+				sb.WriteLine ("enum XamarinGCHandleFlags flags = XamarinGCHandleFlags_None;");
+				sb.WriteLine ("gchandle = xamarin_get_gchandle_with_flags (self, &flags);");
+				sb.WriteLine ("if (gchandle != INVALID_GCHANDLE)");
+				sb.Indent ().WriteLine ("xamarin_set_gchandle_with_flags (self, INVALID_GCHANDLE, XamarinGCHandleFlags_None);").Unindent ();
+			}
 
 			if (!staticCall) {
 				sb.WriteLine ($"static {ucoEntryPoint}_function {ucoEntryPoint};");
@@ -4374,6 +4389,11 @@ void GenerateCallToUnmanagedCallersOnlyMethod (AutoIndentStringBuilder sb, ObjCM
 				GenerateCallToSuperForConstructor (sb, method, exceptions);
 			}
 
+			if (method.CurrentTrampoline == Trampoline.CopyWithZone2) {
+				sb.WriteLine ("if (gchandle != INVALID_GCHANDLE)");
+				sb.Indent ().WriteLine ("xamarin_set_gchandle_with_flags (self, gchandle, flags);").Unindent ();
+			}
+
 			if (hasReturnType)
 				sb.WriteLine ("return rv;");