diff --git a/e2e/cases/stylus/inline-query/index.test.ts b/e2e/cases/stylus/inline-query/index.test.ts
new file mode 100644
index 0000000000..18c3ec4078
--- /dev/null
+++ b/e2e/cases/stylus/inline-query/index.test.ts
@@ -0,0 +1,43 @@
+import { build, dev, rspackOnlyTest } from '@e2e/helper';
+import { expect } from '@playwright/test';
+
+rspackOnlyTest(
+  'should allow to import inline Stylus files in development mode',
+  async ({ page }) => {
+    const rsbuild = await dev({
+      cwd: __dirname,
+      page,
+    });
+
+    const aInline: string = await page.evaluate('window.aInline');
+    const bInline: string = await page.evaluate('window.bInline');
+
+    expect(
+      aInline.includes('.header-class') && aInline.includes('color: red'),
+    ).toBe(true);
+    expect(
+      bInline.includes('.title-class') && bInline.includes('font-size: 14px'),
+    ).toBe(true);
+
+    await rsbuild.close();
+  },
+);
+
+rspackOnlyTest(
+  'should allow to import inline Stylus files in production mode',
+  async ({ page }) => {
+    const rsbuild = await build({
+      cwd: __dirname,
+      page,
+    });
+
+    expect(await page.evaluate('window.aInline')).toBe(
+      '.header-class{color:red}',
+    );
+    expect(await page.evaluate('window.bInline')).toBe(
+      '.title-class{font-size:14px}',
+    );
+
+    await rsbuild.close();
+  },
+);
diff --git a/e2e/cases/stylus/raw/rsbuild.config.ts b/e2e/cases/stylus/inline-query/rsbuild.config.ts
similarity index 100%
rename from e2e/cases/stylus/raw/rsbuild.config.ts
rename to e2e/cases/stylus/inline-query/rsbuild.config.ts
diff --git a/e2e/cases/stylus/raw/src/a.styl b/e2e/cases/stylus/inline-query/src/a.styl
similarity index 100%
rename from e2e/cases/stylus/raw/src/a.styl
rename to e2e/cases/stylus/inline-query/src/a.styl
diff --git a/e2e/cases/stylus/raw/src/b.module.styl b/e2e/cases/stylus/inline-query/src/b.module.styl
similarity index 100%
rename from e2e/cases/stylus/raw/src/b.module.styl
rename to e2e/cases/stylus/inline-query/src/b.module.styl
diff --git a/e2e/cases/stylus/inline-query/src/index.js b/e2e/cases/stylus/inline-query/src/index.js
new file mode 100644
index 0000000000..eedbbf898f
--- /dev/null
+++ b/e2e/cases/stylus/inline-query/src/index.js
@@ -0,0 +1,5 @@
+import aInline from './a.styl?inline';
+import bInline from './b.module.styl?inline';
+
+window.aInline = aInline;
+window.bInline = bInline;
diff --git a/e2e/cases/stylus/raw/index.test.ts b/e2e/cases/stylus/raw-query/index.test.ts
similarity index 100%
rename from e2e/cases/stylus/raw/index.test.ts
rename to e2e/cases/stylus/raw-query/index.test.ts
diff --git a/e2e/cases/stylus/raw-query/rsbuild.config.ts b/e2e/cases/stylus/raw-query/rsbuild.config.ts
new file mode 100644
index 0000000000..accfadda7d
--- /dev/null
+++ b/e2e/cases/stylus/raw-query/rsbuild.config.ts
@@ -0,0 +1,5 @@
+import { pluginStylus } from '@rsbuild/plugin-stylus';
+
+export default {
+  plugins: [pluginStylus()],
+};
diff --git a/e2e/cases/stylus/raw-query/src/a.styl b/e2e/cases/stylus/raw-query/src/a.styl
new file mode 100644
index 0000000000..a608dcd44e
--- /dev/null
+++ b/e2e/cases/stylus/raw-query/src/a.styl
@@ -0,0 +1,3 @@
+.header
+  &-class
+    color red
diff --git a/e2e/cases/stylus/raw-query/src/b.module.styl b/e2e/cases/stylus/raw-query/src/b.module.styl
new file mode 100644
index 0000000000..9319b87565
--- /dev/null
+++ b/e2e/cases/stylus/raw-query/src/b.module.styl
@@ -0,0 +1,3 @@
+.title
+  &-class
+    font-size 14px
diff --git a/e2e/cases/stylus/raw/src/index.js b/e2e/cases/stylus/raw-query/src/index.js
similarity index 100%
rename from e2e/cases/stylus/raw/src/index.js
rename to e2e/cases/stylus/raw-query/src/index.js
diff --git a/packages/core/src/configChain.ts b/packages/core/src/configChain.ts
index 2c6c1a8cc4..f442284003 100644
--- a/packages/core/src/configChain.ts
+++ b/packages/core/src/configChain.ts
@@ -69,6 +69,8 @@ export const CHAIN_ID = {
     STYLUS: 'stylus',
     /** Rule for raw stylus */
     STYLUS_RAW: 'stylus-raw',
+    /** Rule for inline stylus */
+    STYLUS_INLINE: 'stylus-inline',
     /** Rule for svg */
     SVG: 'svg',
     /** Rule for pug */
diff --git a/packages/core/types.d.ts b/packages/core/types.d.ts
index 1ba8ddf13e..d6a37f743a 100644
--- a/packages/core/types.d.ts
+++ b/packages/core/types.d.ts
@@ -203,6 +203,14 @@ declare module '*.less?inline' {
   const content: string;
   export default content;
 }
+declare module '*.styl?inline' {
+  const content: string;
+  export default content;
+}
+declare module '*.stylus?inline' {
+  const content: string;
+  export default content;
+}
 
 /**
  * Raw CSS
diff --git a/packages/plugin-stylus/src/index.ts b/packages/plugin-stylus/src/index.ts
index 6cd64f6336..5d293a72f8 100644
--- a/packages/plugin-stylus/src/index.ts
+++ b/packages/plugin-stylus/src/index.ts
@@ -1,5 +1,5 @@
 import { createRequire } from 'node:module';
-import type { RsbuildPlugin } from '@rsbuild/core';
+import type { RsbuildPlugin, RspackChain } from '@rsbuild/core';
 import deepmerge from 'deepmerge';
 import { reduceConfigs } from 'reduce-configs';
 
@@ -54,11 +54,16 @@ export const pluginStylus = (options?: PluginStylusOptions): RsbuildPlugin => ({
       const rule = chain.module
         .rule(CHAIN_ID.RULE.STYLUS)
         .test(test)
+        // exclude `import './foo.styl?raw'` and `import './foo.stylus?inline'`
+        .resourceQuery({ not: /raw|inline/ })
         .sideEffects(true)
         .resolve.preferRelative(true)
-        .end()
-        // exclude `import './foo.styl?raw'`
-        .resourceQuery({ not: /raw/ });
+        .end();
+
+      const inlineRule = chain.module
+        .rule(CHAIN_ID.RULE.STYLUS_INLINE)
+        .test(test)
+        .resourceQuery(/inline/);
 
       // Support for importing raw Stylus files
       chain.module
@@ -67,27 +72,39 @@ export const pluginStylus = (options?: PluginStylusOptions): RsbuildPlugin => ({
         .type('asset/source')
         .resourceQuery(/raw/);
 
-      // Copy the builtin CSS rules
-      const cssRule = chain.module.rules.get(CHAIN_ID.RULE.CSS);
-      rule.dependency(cssRule.get('dependency'));
+      // Update the normal rule and the inline rule
+      const updateRules = (
+        callback: (rule: RspackChain.Rule, type: 'normal' | 'inline') => void,
+      ) => {
+        callback(rule, 'normal');
+        callback(inlineRule, 'inline');
+      };
 
-      for (const id of Object.keys(cssRule.uses.entries())) {
-        const loader = cssRule.uses.get(id);
-        const options = loader.get('options') ?? {};
-        const clonedOptions = deepmerge<Record<string, any>>({}, options);
+      updateRules((rule, type) => {
+        // Copy the builtin CSS rules
+        const cssRule = chain.module.rules.get(
+          type === 'normal' ? CHAIN_ID.RULE.CSS : CHAIN_ID.RULE.CSS_INLINE,
+        );
+        rule.dependency(cssRule.get('dependency'));
 
-        if (id === CHAIN_ID.USE.CSS) {
-          // add stylus-loader
-          clonedOptions.importLoaders += 1;
-        }
+        for (const id of Object.keys(cssRule.uses.entries())) {
+          const loader = cssRule.uses.get(id);
+          const options = loader.get('options') ?? {};
+          const clonedOptions = deepmerge<Record<string, any>>({}, options);
 
-        rule.use(id).loader(loader.get('loader')).options(clonedOptions);
-      }
+          if (id === CHAIN_ID.USE.CSS) {
+            // add stylus-loader
+            clonedOptions.importLoaders += 1;
+          }
 
-      rule
-        .use(CHAIN_ID.USE.STYLUS)
-        .loader(require.resolve('stylus-loader'))
-        .options(mergedOptions);
+          rule.use(id).loader(loader.get('loader')).options(clonedOptions);
+        }
+
+        rule
+          .use(CHAIN_ID.USE.STYLUS)
+          .loader(require.resolve('stylus-loader'))
+          .options(mergedOptions);
+      });
     });
   },
 });
diff --git a/packages/plugin-stylus/tests/__snapshots__/index.test.ts.snap b/packages/plugin-stylus/tests/__snapshots__/index.test.ts.snap
index 2bfb3a2a3b..b6105ad047 100644
--- a/packages/plugin-stylus/tests/__snapshots__/index.test.ts.snap
+++ b/packages/plugin-stylus/tests/__snapshots__/index.test.ts.snap
@@ -10,7 +10,7 @@ exports[`plugin-stylus > should add stylus loader config correctly 1`] = `
       "preferRelative": true,
     },
     "resourceQuery": {
-      "not": /raw/,
+      "not": /raw\\|inline/,
     },
     "sideEffects": true,
     "test": /\\\\\\.styl\\(us\\)\\?\\$/,
@@ -51,6 +51,38 @@ exports[`plugin-stylus > should add stylus loader config correctly 1`] = `
       },
     ],
   },
+  {
+    "resourceQuery": /inline/,
+    "test": /\\\\\\.styl\\(us\\)\\?\\$/,
+    "use": [
+      {
+        "loader": "<ROOT>/packages/core/compiled/css-loader/index.js",
+        "options": {
+          "exportType": "string",
+          "importLoaders": 2,
+          "modules": false,
+          "sourceMap": false,
+        },
+      },
+      {
+        "loader": "builtin:lightningcss-loader",
+        "options": {
+          "targets": [
+            "chrome >= 87",
+            "edge >= 88",
+            "firefox >= 78",
+            "safari >= 14",
+          ],
+        },
+      },
+      {
+        "loader": "<ROOT>/node_modules/<PNPM_INNER>/stylus-loader/dist/cjs.js",
+        "options": {
+          "sourceMap": false,
+        },
+      },
+    ],
+  },
   {
     "resourceQuery": /raw/,
     "test": /\\\\\\.styl\\(us\\)\\?\\$/,
@@ -69,7 +101,7 @@ exports[`plugin-stylus > should allow to configure stylus options 1`] = `
       "preferRelative": true,
     },
     "resourceQuery": {
-      "not": /raw/,
+      "not": /raw\\|inline/,
     },
     "sideEffects": true,
     "test": /\\\\\\.styl\\(us\\)\\?\\$/,
@@ -113,6 +145,41 @@ exports[`plugin-stylus > should allow to configure stylus options 1`] = `
       },
     ],
   },
+  {
+    "resourceQuery": /inline/,
+    "test": /\\\\\\.styl\\(us\\)\\?\\$/,
+    "use": [
+      {
+        "loader": "<ROOT>/packages/core/compiled/css-loader/index.js",
+        "options": {
+          "exportType": "string",
+          "importLoaders": 2,
+          "modules": false,
+          "sourceMap": false,
+        },
+      },
+      {
+        "loader": "builtin:lightningcss-loader",
+        "options": {
+          "targets": [
+            "chrome >= 87",
+            "edge >= 88",
+            "firefox >= 78",
+            "safari >= 14",
+          ],
+        },
+      },
+      {
+        "loader": "<ROOT>/node_modules/<PNPM_INNER>/stylus-loader/dist/cjs.js",
+        "options": {
+          "sourceMap": false,
+          "stylusOptions": {
+            "lineNumbers": false,
+          },
+        },
+      },
+    ],
+  },
   {
     "resourceQuery": /raw/,
     "test": /\\\\\\.styl\\(us\\)\\?\\$/,