diff --git a/lib/wasix/src/syscalls/wasi/path_open.rs b/lib/wasix/src/syscalls/wasi/path_open.rs
index e3aceea8561..5f8033cb63d 100644
--- a/lib/wasix/src/syscalls/wasi/path_open.rs
+++ b/lib/wasix/src/syscalls/wasi/path_open.rs
@@ -304,6 +304,12 @@ pub(crate) fn path_open_internal(
             if o_flags.contains(Oflags::DIRECTORY) {
                 return Ok(Err(Errno::Notdir));
             }
+
+            // Trailing slash matters. But the underlying opener normalizes it away later.
+            if path.ends_with('/') {
+                return Ok(Err(Errno::Isdir));
+            }
+
             // strip end file name
 
             let (parent_inode, new_entity_name) =
diff --git a/tests/wasi-fyi/fs_open_trailing_slash.rs b/tests/wasi-fyi/fs_open_trailing_slash.rs
index 1493e145fc3..e534d0442a1 100644
--- a/tests/wasi-fyi/fs_open_trailing_slash.rs
+++ b/tests/wasi-fyi/fs_open_trailing_slash.rs
@@ -14,14 +14,18 @@ extern "C" {
 }
 
 const ERRNO_SUCCESS: i32 = 0;
+const ERRNO_ISDIR: i32 = 31;
 const ERRNO_NOTDIR: i32 = 54;
+const OFLAGS_CREAT: i32 = 1;
 const RIGHTS_FD_READ: i64 = 2;
+const RIGHTS_FD_WRITE: i64 = 64;
 
 fn main() {
     unsafe {
         let fd = 5;
         let path_ok = "fyi/fs_open_trailing_slash.dir/file";
         let path_bad = "fyi/fs_open_trailing_slash.dir/file/";
+        let path_bad_new_file = "fyi/fs_open_trailing_slash.dir/new-file/";
         let errno = path_open(
             fd,
             0,
@@ -53,5 +57,21 @@ fn main() {
             errno, ERRNO_NOTDIR,
             "opening a regular file with a trailing slash should fail"
         );
+
+        let errno = path_open(
+            fd,
+            0,
+            path_bad_new_file.as_ptr() as i32,
+            path_bad_new_file.len() as i32,
+            OFLAGS_CREAT,
+            RIGHTS_FD_READ | RIGHTS_FD_WRITE,
+            0,
+            0,
+            1024,
+        );
+        assert_eq!(
+            errno, ERRNO_ISDIR,
+            "creating a regular file with a trailing slash should fail"
+        );
     }
 }