diff --git a/packages/vitest/src/integrations/env/jsdom.ts b/packages/vitest/src/integrations/env/jsdom.ts
index e9a68ffd0937..ef6ed33d6428 100644
--- a/packages/vitest/src/integrations/env/jsdom.ts
+++ b/packages/vitest/src/integrations/env/jsdom.ts
@@ -249,6 +249,10 @@ function createCompatRequest(utils: CompatUtils) {
         super(...args)
       }
     }
+
+    static [Symbol.hasInstance](instance: unknown): boolean {
+      return instance instanceof NodeRequest_
+    }
   }
 }
 
@@ -261,6 +265,10 @@ function createJSDOMCompatURL(utils: CompatUtils): typeof URL {
       }
       return NodeURL.createObjectURL(blob)
     }
+
+    static [Symbol.hasInstance](instance: unknown): boolean {
+      return instance instanceof NodeURL
+    }
   } as typeof URL
 }
 
diff --git a/test/core/test/environments/jsdom.spec.ts b/test/core/test/environments/jsdom.spec.ts
index 158aac3c1eab..b4327bc775c5 100644
--- a/test/core/test/environments/jsdom.spec.ts
+++ b/test/core/test/environments/jsdom.spec.ts
@@ -38,6 +38,9 @@ test('Fetch API accepts other APIs', async () => {
   const searchParams = new URLSearchParams()
   searchParams.set('key', 'value')
   expect.soft(() => new Request('http://localhost', { method: 'POST', body: searchParams })).not.toThrowError()
+
+  const clone = request.clone()
+  expect.soft(clone).toBeInstanceOf(Request)
 })
 
 test('fetch api doesnt override the init object', () => {