diff --git a/go/test/endtoend/vreplication/partial_movetables_seq_test.go b/go/test/endtoend/vreplication/partial_movetables_seq_test.go
index 959a0169950..66cc81dcaff 100644
--- a/go/test/endtoend/vreplication/partial_movetables_seq_test.go
+++ b/go/test/endtoend/vreplication/partial_movetables_seq_test.go
@@ -21,6 +21,7 @@ import (
 	"strings"
 	"testing"
 
+	"github.com/stretchr/testify/assert"
 	"github.com/stretchr/testify/require"
 	"github.com/tidwall/gjson"
 
@@ -38,6 +39,50 @@ import (
 	As part of a separate cleanup we will build on this framework to replace the existing one.
 */
 
+var (
+	seqVSchema = `{
+		"sharded": false,
+		"tables": {
+			"customer_seq": {
+				"type": "sequence"
+			}
+		}
+	}`
+	seqSchema       = `create table customer_seq(id int, next_id bigint, cache bigint, primary key(id)) comment 'vitess_sequence';`
+	commerceSchema  = `create table customer(cid int, name varchar(128), ts timestamp(3) not null default current_timestamp(3), primary key(cid));`
+	commerceVSchema = `
+	{
+	  "tables": {
+		"customer": {}
+	  }
+	}
+`
+	customerSequenceVSchema = `
+	{
+	  "sharded": true,
+	  "vindexes": {
+		"reverse_bits": {
+		  "type": "reverse_bits"
+		}
+	  },
+	  "tables": {
+		"customer": {
+		  "column_vindexes": [
+			{
+			  "column": "cid",
+			  "name": "reverse_bits"
+			}
+		  ],
+		  "auto_increment": {
+			"column": "cid",
+			"sequence": "customer_seq"
+		  }
+		}
+	  }
+	}
+	`
+)
+
 type keyspace struct {
 	name    string
 	vschema string
@@ -74,50 +119,6 @@ type vrepTestCase struct {
 }
 
 func initPartialMoveTablesComplexTestCase(t *testing.T) *vrepTestCase {
-	const (
-		seqVSchema = `{
-			"sharded": false,
-			"tables": {
-				"customer_seq": {
-					"type": "sequence"
-				}
-			}
-		}`
-		seqSchema       = `create table customer_seq(id int, next_id bigint, cache bigint, primary key(id)) comment 'vitess_sequence';`
-		commerceSchema  = `create table customer(cid int, name varchar(128), ts timestamp(3) not null default current_timestamp(3), primary key(cid));`
-		commerceVSchema = `
-		{
-		  "tables": {
-			"customer": {}
-		  }
-		}
-`
-		customerSchema  = ""
-		customerVSchema = `
-		{
-		  "sharded": true,
-		  "vindexes": {
-			"reverse_bits": {
-			  "type": "reverse_bits"
-			}
-		  },
-		  "tables": {
-			"customer": {
-			  "column_vindexes": [
-				{
-				  "column": "cid",
-				  "name": "reverse_bits"
-				}
-			  ],
-			  "auto_increment": {
-				"column": "cid",
-				"sequence": "customer_seq"
-			  }
-			}
-		  }
-		}
-		`
-	)
 	tc := &vrepTestCase{
 		t:               t,
 		testName:        t.Name(),
@@ -134,14 +135,14 @@ func initPartialMoveTablesComplexTestCase(t *testing.T) *vrepTestCase {
 	}
 	tc.keyspaces["customer"] = &keyspace{
 		name:    "customer",
-		vschema: customerVSchema,
-		schema:  customerSchema,
+		vschema: customerSequenceVSchema,
+		schema:  "",
 		baseID:  200,
 		shards:  []string{"-80", "80-"},
 	}
 	tc.keyspaces["customer2"] = &keyspace{
 		name:    "customer2",
-		vschema: customerVSchema,
+		vschema: customerSequenceVSchema,
 		schema:  "",
 		baseID:  1200,
 		shards:  []string{"-80", "80-"},
@@ -165,6 +166,40 @@ func initPartialMoveTablesComplexTestCase(t *testing.T) *vrepTestCase {
 	return tc
 }
 
+func initSequenceResetTestCase(t *testing.T) *vrepTestCase {
+	tc := &vrepTestCase{
+		t:               t,
+		testName:        t.Name(),
+		keyspaces:       make(map[string]*keyspace),
+		defaultCellName: "zone1",
+		workflows:       make(map[string]*workflow),
+	}
+	tc.keyspaces["commerce"] = &keyspace{
+		name:    "commerce",
+		vschema: commerceVSchema,
+		schema:  commerceSchema,
+		baseID:  100,
+		shards:  []string{"0"},
+	}
+	tc.keyspaces["customer"] = &keyspace{
+		name:    "customer",
+		vschema: customerSequenceVSchema,
+		schema:  "",
+		baseID:  200,
+		shards:  []string{"-80", "80-"},
+	}
+	tc.keyspaces["seqSrc"] = &keyspace{
+		name:    "seqSrc",
+		vschema: seqVSchema,
+		schema:  seqSchema,
+		baseID:  400,
+		shards:  []string{"0"},
+	}
+	tc.setupCluster()
+	tc.initData()
+	return tc
+}
+
 func (tc *vrepTestCase) teardown() {
 	tc.vtgateConn.Close()
 	vc.TearDown()
@@ -268,6 +303,93 @@ func (wf *workflow) complete() {
 	require.NoError(wf.tc.t, tstWorkflowExec(wf.tc.t, wf.tc.defaultCellName, wf.name, wf.fromKeyspace, wf.toKeyspace, "", workflowActionComplete, "", "", "", defaultWorkflowExecOptions))
 }
 
+// TestSequenceResetOnSwitchTraffic tests that in-memory sequence info is
+// reset when switching traffic back and forth between keyspaces during
+// MoveTables workflow. This catches a bug where cached sequence values would
+// persist after traffic switches, causing sequence generation to produce
+// duplicate values in target keyspace.
+func TestSequenceResetOnSwitchTraffic(t *testing.T) {
+	origExtraVTGateArgs := extraVTGateArgs
+	extraVTGateArgs = append(extraVTGateArgs, []string{
+		"--enable-partial-keyspace-migration",
+		"--schema_change_signal=false",
+	}...)
+	defer func() {
+		extraVTGateArgs = origExtraVTGateArgs
+	}()
+
+	tc := initSequenceResetTestCase(t)
+	defer tc.teardown()
+
+	currentCustomerCount = getCustomerCount(t, "")
+	newCustomerCount = 4
+	t.Run("Verify sequence reset during traffic switching", func(t *testing.T) {
+		tc.setupKeyspaces([]string{"customer"})
+		wf := tc.newWorkflow("MoveTables", "customer", "commerce", "customer", &workflowOptions{
+			tables: []string{"customer"},
+		})
+		wf.create()
+
+		vtgateConn, closeConn := getVTGateConn()
+		defer closeConn()
+
+		getSequenceNextID := func() int64 {
+			qr := execVtgateQuery(t, vtgateConn, "", "SELECT next_id FROM seqSrc.customer_seq WHERE id = 0")
+			nextID, _ := qr.Rows[0][0].ToInt64()
+			return nextID
+		}
+
+		initialSeqValue := getSequenceNextID()
+		t.Logf("Initial sequence next_id: %d", initialSeqValue)
+
+		wf.switchTraffic()
+
+		insertCustomers(t)
+
+		afterFirstSwitchSeqValue := getSequenceNextID()
+		t.Logf("After first switch sequence next_id: %d", afterFirstSwitchSeqValue)
+		require.Greater(t, afterFirstSwitchSeqValue, initialSeqValue, "Sequence should increment after inserting customers")
+
+		wf.reverseTraffic()
+
+		afterReverseSeqValue := getSequenceNextID()
+		t.Logf("After reverse switch sequence next_id: %d", afterReverseSeqValue)
+
+		// Insert some random values when all writes are reversed back to
+		// source keyspace. We are inserting here rows with IDs 1004, 1005,
+		// 1006 (since the cache value was 1000) which would be the next
+		// in-memory sequence IDs for inserting any new rows in `customer`
+		// table if the sequence info isn't reset. This will result in
+		// duplicate primary key value error in the next insert.
+		//
+		// Hence, this way we verify that even if there are any new
+		// values inserted after the traffic has been reversed, the in-memory
+		// sequence info is reset, so that on switching back the traffic to
+		// the target keyspace the tablet refetches the next_id from the
+		// sequence table for generating the next insert ID.
+		_, err := tc.vtgateConn.ExecuteFetch("insert into customer(cid, name) values(1004, 'customer8'), (1005, 'customer9'),(1006, 'customer10')", 1000, false)
+		require.NoError(t, err)
+		_, err = tc.vtgateConn.ExecuteFetch("insert into customer(cid, name) values(2004, 'customer11'), (2005, 'customer12'),(2006, 'customer13')", 1000, false)
+		require.NoError(t, err)
+
+		wf.switchTraffic()
+
+		afterSecondSwitchSeqValue := getSequenceNextID()
+		// Since the highest ID before switching traffic was 2026, which is
+		// greater than 2000 (the expected next_id from sequence table before switch.)
+		assert.Equal(t, int64(2007), afterSecondSwitchSeqValue)
+
+		currentCustomerCount = getCustomerCount(t, "after second switch")
+		newCustomerCount = 4
+		insertCustomers(t)
+
+		finalSeqValue := getSequenceNextID()
+		assert.Equal(t, int64(3007), finalSeqValue, "Since the cache is set to 1000, next_id is expected to be incremented to 3007")
+
+		wf.complete()
+	})
+}
+
 // TestPartialMoveTablesWithSequences enhances TestPartialMoveTables by adding an unsharded keyspace which has a
 // sequence. This tests that the sequence is migrated correctly and that we can reverse traffic back to the source
 func TestPartialMoveTablesWithSequences(t *testing.T) {
@@ -324,7 +446,6 @@ func TestPartialMoveTablesWithSequences(t *testing.T) {
 	targetKs := "customer2"
 	shard := "80-"
 	var wf80Dash, wfDash80 *workflow
-	currentCustomerCount = getCustomerCount(t, "before customer2.80-")
 	vtgateConn, closeConn := getVTGateConn()
 	t.Run("Start MoveTables on customer2.80-", func(t *testing.T) {
 		// Now setup the customer2 keyspace so we can do a partial move tables for one of the two shards: 80-.
@@ -336,14 +457,11 @@ func TestPartialMoveTablesWithSequences(t *testing.T) {
 		})
 		wf80Dash.create()
 
-		currentCustomerCount = getCustomerCount(t, "after customer2.80-")
 		waitForRowCount(t, vtgateConn, "customer2:80-", "customer", 2) // customer2: 80-
 		waitForRowCount(t, vtgateConn, "customer", "customer", 3)      // customer: all shards
 		waitForRowCount(t, vtgateConn, "customer2", "customer", 3)     // customer2: all shards
 	})
 
-	currentCustomerCount = getCustomerCount(t, "after customer2.80-/2")
-
 	// This query uses an ID that should always get routed to shard 80-
 	shard80DashRoutedQuery := "select name from customer where cid = 1 and noexistcol = 'foo'"
 	// This query uses an ID that should always get routed to shard -80
@@ -382,14 +500,12 @@ func TestPartialMoveTablesWithSequences(t *testing.T) {
 
 		_, err = vtgateConn.ExecuteFetch("use `customer`", 0, false) // switch vtgate default db back to customer
 		require.NoError(t, err)
-		currentCustomerCount = getCustomerCount(t, "")
 
 		// Switch all traffic for the shard
 		wf80Dash.switchTraffic()
 		expectedSwitchOutput := fmt.Sprintf("SwitchTraffic was successful for workflow %s.%s\n\nStart State: Reads Not Switched. Writes Not Switched\nCurrent State: Reads partially switched, for shards: %s. Writes partially switched, for shards: %s\n\n",
 			targetKs, wfName, shard, shard)
 		require.Equal(t, expectedSwitchOutput, lastOutput)
-		currentCustomerCount = getCustomerCount(t, "")
 
 		// Confirm global routing rules -- everything should still be routed
 		// to the source side, customer, globally.
@@ -431,7 +547,6 @@ func TestPartialMoveTablesWithSequences(t *testing.T) {
 		_, err = vtgateConn.ExecuteFetch("use `customer`", 0, false) // switch vtgate default db back to customer
 		require.NoError(t, err)
 	})
-	currentCustomerCount = getCustomerCount(t, "")
 
 	// Now move the other shard: -80
 	t.Run("Move shard -80 and validate routing rules", func(t *testing.T) {
diff --git a/go/vt/vtctl/workflow/sequences.go b/go/vt/vtctl/workflow/sequences.go
index 2ef83013e94..b2f6e8e70e0 100644
--- a/go/vt/vtctl/workflow/sequences.go
+++ b/go/vt/vtctl/workflow/sequences.go
@@ -290,6 +290,13 @@ func (ts *trafficSwitcher) updateSequenceValue(ctx context.Context, seq *sequenc
 			MaxRows: 1,
 		})
 		if err == nil {
+			// It is important to reset in-memory sequence counters on the table,
+			// since it is possible for it to be outdated, this will prevent duplicate
+			// key errors.
+			err := ts.TabletManagerClient().ResetSequences(ctx, sequenceTablet.Tablet, []string{seq.backingTableName})
+			if err != nil {
+				return vterrors.Errorf(vtrpcpb.Code_INTERNAL, "failed to reset sequences on %q: %v", seq.backingTableKeyspace, err)
+			}
 			return nil
 		}
 
diff --git a/test/config.json b/test/config.json
index 0bd874af373..a7135c4c13f 100644
--- a/test/config.json
+++ b/test/config.json
@@ -1209,6 +1209,15 @@
 			"RetryMax": 1,
 			"Tags": []
 		},
+		"vreplication_sequence_reset_on_switch_traffic": {
+			"File": "unused.go",
+			"Args": ["vitess.io/vitess/go/test/endtoend/vreplication", "-run", "TestSequenceResetOnSwitchTraffic"],
+			"Command": [],
+			"Manual": false,
+			"Shard": "vreplication_partial_movetables_and_materialize",
+			"RetryMax": 1,
+			"Tags": []
+		},
 		"vstream_flush_binlog": {
 			"File": "unused.go",
 			"Args": ["vitess.io/vitess/go/test/endtoend/vreplication", "-run", "TestVStreamFlushBinlog"],