diff --git a/packages/vite/src/node/__tests__/plugins/workerImportMetaUrl.spec.ts b/packages/vite/src/node/__tests__/plugins/workerImportMetaUrl.spec.ts
index a6d60a84b345f3..348e3e39be0147 100644
--- a/packages/vite/src/node/__tests__/plugins/workerImportMetaUrl.spec.ts
+++ b/packages/vite/src/node/__tests__/plugins/workerImportMetaUrl.spec.ts
@@ -221,4 +221,36 @@ new Worker(
       `"repro(new Worker(new URL(/* @vite-ignore */ "/worker?worker_file&type=classic", '' + import.meta.url)), { type: "module" })"`,
     )
   })
+
+  test('with multi-line new URL and trailing comma', async () => {
+    expect(
+      await transform(`new Worker(
+  new URL(
+    "./worker.js",
+    import.meta.url,
+  )
+)`),
+    ).toMatchInlineSnapshot(`
+      "new Worker(
+        new URL(/* @vite-ignore */ "/worker.js?worker_file&type=classic", '' + import.meta.url)
+      )"
+    `)
+  })
+
+  test('with multi-line new URL, trailing comma, and worker options', async () => {
+    expect(
+      await transform(`const worker = new Worker(
+  new URL(
+    "./worker.js",
+    import.meta.url,
+  ),
+  { type: "module" },
+)`),
+    ).toMatchInlineSnapshot(`
+      "const worker = new Worker(
+        new URL(/* @vite-ignore */ "/worker.js?worker_file&type=module", '' + import.meta.url),
+        { type: "module" },
+      )"
+    `)
+  })
 })
diff --git a/packages/vite/src/node/plugins/workerImportMetaUrl.ts b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
index a8b25a262ff0f4..991783b64e075b 100644
--- a/packages/vite/src/node/plugins/workerImportMetaUrl.ts
+++ b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
@@ -209,7 +209,7 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
         let s: MagicString | undefined
         const cleanString = stripLiteral(code)
         const workerImportMetaUrlRE =
-          /\bnew\s+(?:Worker|SharedWorker)\s*\(\s*(new\s+URL\s*\(\s*('[^']+'|"[^"]+"|`[^`]+`)\s*,\s*import\.meta\.url\s*\))/dg
+          /\bnew\s+(?:Worker|SharedWorker)\s*\(\s*(new\s+URL\s*\(\s*('[^']+'|"[^"]+"|`[^`]+`)\s*,\s*import\.meta\.url\s*(?:,\s*)?\))/dg
 
         let match: RegExpExecArray | null
         while ((match = workerImportMetaUrlRE.exec(cleanString))) {