diff --git a/packages/vite/src/node/plugins/workerImportMetaUrl.ts b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
index a8b25a262ff0f4..991783b64e075b 100644
--- a/packages/vite/src/node/plugins/workerImportMetaUrl.ts
+++ b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
@@ -209,7 +209,7 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
         let s: MagicString | undefined
         const cleanString = stripLiteral(code)
         const workerImportMetaUrlRE =
-          /\bnew\s+(?:Worker|SharedWorker)\s*\(\s*(new\s+URL\s*\(\s*('[^']+'|"[^"]+"|`[^`]+`)\s*,\s*import\.meta\.url\s*\))/dg
+          /\bnew\s+(?:Worker|SharedWorker)\s*\(\s*(new\s+URL\s*\(\s*('[^']+'|"[^"]+"|`[^`]+`)\s*,\s*import\.meta\.url\s*(?:,\s*)?\))/dg
 
         let match: RegExpExecArray | null
         while ((match = workerImportMetaUrlRE.exec(cleanString))) {
diff --git a/playground/worker/__tests__/es/worker-es.spec.ts b/playground/worker/__tests__/es/worker-es.spec.ts
index 95282d30fa27fb..e24ef67646c0e2 100644
--- a/playground/worker/__tests__/es/worker-es.spec.ts
+++ b/playground/worker/__tests__/es/worker-es.spec.ts
@@ -148,6 +148,12 @@ test('module worker', async () => {
     .toMatch('A string')
 })
 
+test('new URL(..., import.meta.url) (multiline with trailing comma)', async () => {
+  await expect
+    .poll(() => page.textContent('.worker-import-meta-url-multiline'))
+    .toMatch('A string')
+})
+
 test('classic worker', async () => {
   await expect
     .poll(() => page.textContent('.classic-worker'))
diff --git a/playground/worker/__tests__/iife/worker-iife.spec.ts b/playground/worker/__tests__/iife/worker-iife.spec.ts
index 07ae7ab0bc9221..645c55d90f9061 100644
--- a/playground/worker/__tests__/iife/worker-iife.spec.ts
+++ b/playground/worker/__tests__/iife/worker-iife.spec.ts
@@ -133,6 +133,12 @@ test('module worker', async () => {
     .toMatch('A string')
 })
 
+test('new URL(..., import.meta.url) (multiline with trailing comma)', async () => {
+  await expect
+    .poll(() => page.textContent('.worker-import-meta-url-multiline'))
+    .toMatch(/A\sstring.*\/iife\/.+url-worker\.js.+url-worker\.js/)
+})
+
 test('classic worker', async () => {
   await expect
     .poll(() => page.textContent('.classic-worker'))
diff --git a/playground/worker/index.html b/playground/worker/index.html
index 8ece3711324053..e26b4cdfad39cf 100644
--- a/playground/worker/index.html
+++ b/playground/worker/index.html
@@ -88,6 +88,12 @@ <h2 class="format-iife">format iife:</h2>
 </p>
 <code class="worker-import-meta-url"></code>
 
+<p>
+  new Worker(new URL(..., import.meta.url,), ...) (multiline with trailing comma)
+  <span class="classname">.worker-import-meta-url-multiline</span>
+</p>
+<code class="worker-import-meta-url-multiline"></code>
+
 <p>
   new Worker(new URL('@/url-worker', import.meta.url), { type: 'module' })
   <span class="classname">.worker-import-meta-url-resolve</span>
diff --git a/playground/worker/worker/main-module.js b/playground/worker/worker/main-module.js
index a659aa438fd5ca..3ea13fba7fbdc4 100644
--- a/playground/worker/worker/main-module.js
+++ b/playground/worker/worker/main-module.js
@@ -118,6 +118,18 @@ w.addEventListener('message', (ev) =>
   text('.worker-import-meta-url', JSON.stringify(ev.data)),
 )
 
+// url import worker (multiline with trailing comma - formatted by prettier)
+const wMultiline = new Worker(
+  new URL(
+    '../url-worker.js',
+    import.meta.url,
+  ),
+  { type: 'module' },
+)
+wMultiline.addEventListener('message', (ev) =>
+  text('.worker-import-meta-url-multiline', JSON.stringify(ev.data)),
+)
+
 // url import worker with alias path
 const wResolve = new Worker(
   new URL('@/url-worker.js', import.meta.url),