Command line: /home/denis/miniconda3/envs/S_pades/bin/spades.py	--only-assembler	-1	/home/denis/data/Pot/R_GCTCATGA-TCTTACGC_L004_R1_001.fastq.gz	-2	/home/denis/data/Pot/R_GCTCATGA-TCTTACGC_L004_R2_001.fastq.gz	--nanopore	/home/denis/data/Pot_Spades_assembly/ONT_total.fq	--isolate	-t	15	-m	200	-o	/home/denis/data/Pot_Spades_assembly/Spades_PA_out	-t	35	-m	450
Restart-from=last
with updated parameters: -t	35	-m	450

System information:
  SPAdes version: 4.0.0
  Python version: 3.13.1
  OS: Linux-5.15.0-125-generic-x86_64-with-glibc2.35

Output dir: /home/denis/data/Pot_Spades_assembly/Spades_PA_out
Mode: ONLY assembling (without read error correction)
Debug mode is turned OFF

Dataset parameters:
  Isolate mode
  Reads:
    Library number: 1, library type: paired-end
      orientation: fr
      left reads: ['/home/denis/data/Pot/R_GCTCATGA-TCTTACGC_L004_R1_001.fastq.gz']
      right reads: ['/home/denis/data/Pot/R_GCTCATGA-TCTTACGC_L004_R2_001.fastq.gz']
      interlaced reads: not specified
      single reads: not specified
      merged reads: not specified
    Library number: 2, library type: nanopore
      left reads: not specified
      right reads: not specified
      interlaced reads: not specified
      single reads: ['/home/denis/data/Pot_Spades_assembly/ONT_total.fq']
      merged reads: not specified
Assembly parameters:
  k: automatic selection based on read length
  Repeat resolution is enabled
  Mismatch careful mode is turned OFF
  MismatchCorrector will be SKIPPED
  Coverage cutoff is turned OFF
  Assembly graph output will use GFA v1.2 format
Other parameters:
  Dir for temp files: /home/denis/data/Pot_Spades_assembly/Spades_PA_out/tmp
  Threads: 35
  Memory limit (in Gb): 450