diff --git a/TUnit.Assertions.SourceGenerator.Tests/MethodAssertionGeneratorTests.cs b/TUnit.Assertions.SourceGenerator.Tests/MethodAssertionGeneratorTests.cs
index 5f975588103..565893474b6 100644
--- a/TUnit.Assertions.SourceGenerator.Tests/MethodAssertionGeneratorTests.cs
+++ b/TUnit.Assertions.SourceGenerator.Tests/MethodAssertionGeneratorTests.cs
@@ -201,4 +201,37 @@ public Task FileScopedClassWithInlining() => RunTest(
             // Snapshot test - the actual verification is done by snapshot comparison
             await Assert.That(generatedFiles.Count).IsGreaterThanOrEqualTo(1);
         });
+
+#if NET6_0_OR_GREATER
+    [Test]
+    public Task RefStructParameter() => RunTest(
+        Path.Combine(Sourcy.Git.RootDirectory.FullName,
+            "TUnit.Assertions.SourceGenerator.Tests",
+            "TestData",
+            "RefStructParameterAssertion.cs"),
+        async generatedFiles =>
+        {
+            await Assert.That(generatedFiles).HasCount(1);
+
+            var mainFile = generatedFiles.First();
+            await Assert.That(mainFile).IsNotNull();
+
+            // Verify that the field type is string, not the ref struct
+            await Assert.That(mainFile).Contains("private readonly string _message;");
+            await Assert.That(mainFile).Contains("private readonly string _suffix;");
+
+            // Verify that the extension method converts the ref struct to string
+            await Assert.That(mainFile).Contains("message.ToStringAndClear()");
+            await Assert.That(mainFile).Contains("suffix.ToStringAndClear()");
+
+            // Verify the constructor takes string, not the ref struct
+            await Assert.That(mainFile).Contains("string message)");
+            await Assert.That(mainFile).Contains("string suffix)");
+
+            // Verify that .ToStringAndClear() is removed in the inlined body
+            // (since the field is already a string)
+            // The inlined body should use _message directly, not _message.ToStringAndClear()
+            await Assert.That(mainFile).Contains("value!.Contains(_message)");
+        });
+#endif
 }
diff --git a/TUnit.Assertions.SourceGenerator.Tests/TestData/RefStructParameterAssertion.cs b/TUnit.Assertions.SourceGenerator.Tests/TestData/RefStructParameterAssertion.cs
new file mode 100644
index 00000000000..6fa3559fa05
--- /dev/null
+++ b/TUnit.Assertions.SourceGenerator.Tests/TestData/RefStructParameterAssertion.cs
@@ -0,0 +1,30 @@
+#if NET6_0_OR_GREATER
+using System.Runtime.CompilerServices;
+using TUnit.Assertions.Attributes;
+
+namespace TUnit.Assertions.Tests.TestData;
+
+/// <summary>
+/// Test case: Method with ref struct parameter (DefaultInterpolatedStringHandler)
+/// The generator should convert the ref struct to string before storing it
+/// </summary>
+public static class RefStructParameterAssertions
+{
+    /// <summary>
+    /// Test that interpolated string handlers are properly converted to strings
+    /// </summary>
+    [GenerateAssertion(ExpectationMessage = "to contain {message}", InlineMethodBody = true)]
+    public static bool ContainsMessage(this string value, ref DefaultInterpolatedStringHandler message)
+    {
+        var stringMessage = message.ToStringAndClear();
+        return value.Contains(stringMessage);
+    }
+
+    /// <summary>
+    /// Test with a simpler expression body
+    /// </summary>
+    [GenerateAssertion(ExpectationMessage = "to end with {suffix}", InlineMethodBody = true)]
+    public static bool EndsWithMessage(this string value, ref DefaultInterpolatedStringHandler suffix)
+        => value.EndsWith(suffix.ToStringAndClear());
+}
+#endif
diff --git a/TUnit.Assertions.SourceGenerator/Generators/MethodAssertionGenerator.cs b/TUnit.Assertions.SourceGenerator/Generators/MethodAssertionGenerator.cs
index 177cc51d831..95bbfcae85f 100644
--- a/TUnit.Assertions.SourceGenerator/Generators/MethodAssertionGenerator.cs
+++ b/TUnit.Assertions.SourceGenerator/Generators/MethodAssertionGenerator.cs
@@ -47,6 +47,15 @@ public sealed class MethodAssertionGenerator : IIncrementalGenerator
         isEnabledByDefault: true,
         description: "Methods decorated with [GenerateAssertion] must return bool, AssertionResult, Task<bool>, or Task<AssertionResult>.");
 
+    private static readonly DiagnosticDescriptor RefStructRequiresInliningRule = new DiagnosticDescriptor(
+        id: "TUNITGEN004",
+        title: "Ref struct parameter requires method body inlining",
+        messageFormat: "Method '{0}' has ref struct parameter '{1}' of type '{2}'. Use InlineMethodBody = true and ensure the method has a single-expression or single-return-statement body",
+        category: "TUnit.Assertions.SourceGenerator",
+        defaultSeverity: DiagnosticSeverity.Error,
+        isEnabledByDefault: true,
+        description: "Methods with ref struct parameters (like DefaultInterpolatedStringHandler) require InlineMethodBody = true because ref structs cannot be stored as class fields. The method must have a simple body that can be inlined.");
+
     public void Initialize(IncrementalGeneratorInitializationContext context)
     {
         // Find all methods decorated with [GenerateAssertion]
@@ -259,6 +268,22 @@ private static (AssertionMethodData? Data, Diagnostic? Diagnostic) GetAssertionM
             }
         }
 
+        // Validate that methods with ref struct parameters have inlined method bodies
+        // Ref structs cannot be stored as class fields, so we need to inline the method body
+        foreach (var param in additionalParameters)
+        {
+            if (IsRefStruct(param.Type) && string.IsNullOrEmpty(methodBody))
+            {
+                var diagnostic = Diagnostic.Create(
+                    RefStructRequiresInliningRule,
+                    location,
+                    methodSymbol.Name,
+                    param.Name,
+                    param.Type.ToDisplayString());
+                return (null, diagnostic);
+            }
+        }
+
         var data = new AssertionMethodData(
             methodSymbol,
             targetType,
@@ -545,9 +570,11 @@ private static void GenerateAssertionClass(StringBuilder sb, AssertionMethodData
         sb.AppendLine("{");
 
         // Private fields for additional parameters
+        // Note: Ref struct types (like DefaultInterpolatedStringHandler) are stored as string
         foreach (var param in data.AdditionalParameters)
         {
-            sb.AppendLine($"    private readonly {param.Type.ToDisplayString()} _{param.Name};");
+            var fieldType = IsRefStruct(param.Type) ? "string" : param.Type.ToDisplayString();
+            sb.AppendLine($"    private readonly {fieldType} _{param.Name};");
         }
 
         if (data.AdditionalParameters.Length > 0)
@@ -556,10 +583,12 @@ private static void GenerateAssertionClass(StringBuilder sb, AssertionMethodData
         }
 
         // Constructor
+        // Note: Ref struct parameters are received as string (pre-converted by extension method)
         sb.Append($"    public {className}(AssertionContext<{targetTypeName}> context");
         foreach (var param in data.AdditionalParameters)
         {
-            sb.Append($", {param.Type.ToDisplayString()} {param.Name}");
+            var paramType = IsRefStruct(param.Type) ? "string" : param.Type.ToDisplayString();
+            sb.Append($", {paramType} {param.Name}");
         }
         sb.AppendLine(")");
         sb.AppendLine("        : base(context)");
@@ -730,6 +759,26 @@ private static string BuildInlinedExpression(AssertionMethodData data)
                 $"_{paramName}");
         }
 
+        // For ref struct parameters that have been converted to strings,
+        // remove calls to .ToStringAndClear() and .ToString() since the value is already a string
+        foreach (var param in data.AdditionalParameters)
+        {
+            if (IsRefStruct(param.Type))
+            {
+                var fieldName = $"_{param.Name}";
+                // Remove .ToStringAndClear() - the value is already a string
+                inlinedBody = Regex.Replace(
+                    inlinedBody,
+                    $@"{Regex.Escape(fieldName)}\.ToStringAndClear\(\)",
+                    fieldName);
+                // Remove .ToString() - the value is already a string
+                inlinedBody = Regex.Replace(
+                    inlinedBody,
+                    $@"{Regex.Escape(fieldName)}\.ToString\(\)",
+                    fieldName);
+            }
+        }
+
         // Add null-forgiving operator for reference types if not already present
         // This is safe because we've already checked for null above
         var isNullable = data.TargetType.IsReferenceType || data.TargetType.NullableAnnotation == NullableAnnotation.Annotated;
@@ -873,10 +922,23 @@ private static void GenerateExtensionMethod(StringBuilder sb, AssertionMethodDat
         }
 
         // Construct and return assertion
+        // Note: Ref struct parameters (like interpolated string handlers) are converted to string
         sb.Append($"        return new {className}{genericDeclaration}(source.Context");
         foreach (var param in data.AdditionalParameters)
         {
-            sb.Append($", {param.Name}");
+            if (IsRefStruct(param.Type))
+            {
+                // Convert ref struct to string - use ToStringAndClear for interpolated string handlers
+                // or ToString() for other ref structs
+                var conversion = IsInterpolatedStringHandler(param.Type)
+                    ? $"{param.Name}.ToStringAndClear()"
+                    : $"{param.Name}.ToString()";
+                sb.Append($", {conversion}");
+            }
+            else
+            {
+                sb.Append($", {param.Name}");
+            }
         }
         sb.AppendLine(");");
 
@@ -1001,6 +1063,61 @@ private static List<string> CollectGenericConstraints(IMethodSymbol method)
         return constraints;
     }
 
+    /// <summary>
+    /// Checks if a type is a ref struct (ref-like type).
+    /// Ref structs cannot be stored as fields in classes.
+    /// </summary>
+    private static bool IsRefStruct(ITypeSymbol type)
+    {
+        if (type is not INamedTypeSymbol namedType)
+        {
+            return false;
+        }
+
+        // Use reflection to access IsRefLikeType property which may not be available in all Roslyn versions
+        var isRefLikeProperty = namedType.GetType().GetProperty("IsRefLikeType");
+        if (isRefLikeProperty?.GetValue(namedType) is bool isRefLike && isRefLike)
+        {
+            return true;
+        }
+
+        // Fallback: check for common ref struct types by name
+        var typeName = namedType.ToDisplayString();
+        if (typeName.StartsWith("System.Span<") ||
+            typeName.StartsWith("System.ReadOnlySpan<") ||
+            typeName == "System.Runtime.CompilerServices.DefaultInterpolatedStringHandler")
+        {
+            return true;
+        }
+
+        // Check for InterpolatedStringHandlerAttribute on the type
+        return namedType.GetAttributes().Any(attr =>
+            attr.AttributeClass?.ToDisplayString() == "System.Runtime.CompilerServices.InterpolatedStringHandlerAttribute");
+    }
+
+    /// <summary>
+    /// Checks if a type is an interpolated string handler (e.g., DefaultInterpolatedStringHandler).
+    /// These types need special handling as they should be converted to string.
+    /// </summary>
+    private static bool IsInterpolatedStringHandler(ITypeSymbol type)
+    {
+        if (type is not INamedTypeSymbol namedType)
+        {
+            return false;
+        }
+
+        // Check for DefaultInterpolatedStringHandler specifically
+        var typeName = namedType.ToDisplayString();
+        if (typeName == "System.Runtime.CompilerServices.DefaultInterpolatedStringHandler")
+        {
+            return true;
+        }
+
+        // Check for InterpolatedStringHandlerAttribute on the type
+        return namedType.GetAttributes().Any(attr =>
+            attr.AttributeClass?.ToDisplayString() == "System.Runtime.CompilerServices.InterpolatedStringHandlerAttribute");
+    }
+
     private enum ReturnTypeKind
     {
         Bool,