OSError: Unable to synchronously open file (truncated file: eof = 7084411, sblock->base_addr = 0, stored_eof = 196566066)

[wrab12]

Error Description

When running the following code from the Spatiotemporal trajectory of mouse organogenesis tutorial:

adata = datasets.mosta()
adata.obs["time"] = adata.obs["time"].astype("category")
adata

Solution:

1. Navigate to the cache directory:

   • Path: C:\Users\{username}\.cache\moscot\mosta.h5ad

2. Delete the mosta.h5ad file

3. Rerun the code, which will trigger a fresh download

Why This Works

• The existing file is likely corrupted or incompletely downloaded
• Deleting the file forces moscot to download a fresh, complete dataset

Note: Replace {username} with your actual Windows username.

Alternative Approach

If deletion doesn't resolve the issue, you can also:

• Check your internet connection
• Manually download the file from the original source
• Verify you have the latest versions of moscot, h5py, and anndata