diff --git a/code/addons/vitest/package.json b/code/addons/vitest/package.json
index ce6482ce27ab..480654bcdb2f 100644
--- a/code/addons/vitest/package.json
+++ b/code/addons/vitest/package.json
@@ -107,11 +107,11 @@
     "vitest": "^4.1.6"
   },
   "peerDependencies": {
-    "@vitest/browser": "^3.0.0 || ^4.0.0",
-    "@vitest/browser-playwright": "^4.0.0",
-    "@vitest/runner": "^3.0.0 || ^4.0.0",
+    "@vitest/browser": "^3.0.0 || ^4.0.0 || ^5.0.0",
+    "@vitest/browser-playwright": "^4.0.0 || ^5.0.0",
+    "@vitest/runner": "^3.0.0 || ^4.0.0 || ^5.0.0",
     "storybook": "workspace:^",
-    "vitest": "^3.0.0 || ^4.0.0"
+    "vitest": "^3.0.0 || ^4.0.0 || ^5.0.0"
   },
   "peerDependenciesMeta": {
     "@vitest/browser": {
diff --git a/code/addons/vitest/src/constants.ts b/code/addons/vitest/src/constants.ts
index b1fbfdf1ece4..69d510194efb 100644
--- a/code/addons/vitest/src/constants.ts
+++ b/code/addons/vitest/src/constants.ts
@@ -71,6 +71,7 @@ export const STORYBOOK_TEST_PROVIDE_KEY = 'storybook/test-provided';
 export const STORYBOOK_CORE_GHOST_STORIES_PROVIDE_KEY = 'storybook/core-ghost-stories';
 export const STORYBOOK_CORE_RENDER_ANALYSIS_PROVIDE_KEY = 'storybook/core-render-analysis';
 export const STORYBOOK_TEST_INITIAL_GLOBALS_PROVIDE_KEY = 'storybook/test-initial-globals';
+export const STORYBOOK_TEST_FEATURES_PROVIDE_KEY = 'storybook/test-features';
 
 // Channel event names for programmatic test triggering
 export const TRIGGER_TEST_RUN_REQUEST = `${ADDON_ID}/trigger-test-run-request`;
diff --git a/code/addons/vitest/src/vitest-plugin/index.ts b/code/addons/vitest/src/vitest-plugin/index.ts
index 9d11c6cba22f..b61ef4b86ec5 100644
--- a/code/addons/vitest/src/vitest-plugin/index.ts
+++ b/code/addons/vitest/src/vitest-plugin/index.ts
@@ -44,6 +44,7 @@ import { withoutVitePlugins } from '../../../../builders/builder-vite/src/utils/
 import {
   STORYBOOK_CORE_GHOST_STORIES_PROVIDE_KEY,
   STORYBOOK_CORE_RENDER_ANALYSIS_PROVIDE_KEY,
+  STORYBOOK_TEST_FEATURES_PROVIDE_KEY,
   STORYBOOK_TEST_INITIAL_GLOBALS_PROVIDE_KEY,
 } from '../constants.ts';
 import type { InternalOptions, UserOptions } from './types.ts';
@@ -391,6 +392,7 @@ export const storybookTest = async (options?: UserOptions): Promise<Plugin[]> =>
           },
 
           provide: {
+            [STORYBOOK_TEST_FEATURES_PROVIDE_KEY]: features,
             [STORYBOOK_CORE_GHOST_STORIES_PROVIDE_KEY]: !!process.env.STORYBOOK_COMPONENT_PATHS,
             [STORYBOOK_CORE_RENDER_ANALYSIS_PROVIDE_KEY]:
               !!process.env.STORYBOOK_COMPONENT_PATHS || withinAgenticSetupSession,
diff --git a/code/addons/vitest/src/vitest-plugin/setup-file.ts b/code/addons/vitest/src/vitest-plugin/setup-file.ts
index d8a02e183d4c..466816172a92 100644
--- a/code/addons/vitest/src/vitest-plugin/setup-file.ts
+++ b/code/addons/vitest/src/vitest-plugin/setup-file.ts
@@ -1,10 +1,17 @@
-import { afterEach, beforeAll, vi } from 'vitest';
+import { afterEach, beforeAll, beforeEach, inject, vi } from 'vitest';
 import type { RunnerTask } from 'vitest';
 
 import { Channel } from 'storybook/internal/channels';
 import { getChannel, setChannel } from 'storybook/internal/channels';
 
-import { COMPONENT_TESTING_PANEL_ID } from '../constants.ts';
+import { COMPONENT_TESTING_PANEL_ID, STORYBOOK_TEST_FEATURES_PROVIDE_KEY } from '../constants.ts';
+
+// Vitest 5 replaces the Vite define without exposing it on the runtime global.
+const features = inject(STORYBOOK_TEST_FEATURES_PROVIDE_KEY);
+if (features) {
+  vi.stubGlobal('FEATURES', features);
+  beforeEach(() => vi.stubGlobal('FEATURES', features));
+}
 
 declare global {
   // eslint-disable-next-line @typescript-eslint/ban-ts-comment
diff --git a/code/addons/vitest/src/vitest-provided-context.d.ts b/code/addons/vitest/src/vitest-provided-context.d.ts
index 352ef54d885f..8bd9a640971f 100644
--- a/code/addons/vitest/src/vitest-provided-context.d.ts
+++ b/code/addons/vitest/src/vitest-provided-context.d.ts
@@ -1,14 +1,18 @@
 import 'vitest';
 
+import type { StorybookConfigRaw } from 'storybook/internal/types';
+
 import type {
   STORYBOOK_CORE_GHOST_STORIES_PROVIDE_KEY,
   STORYBOOK_CORE_RENDER_ANALYSIS_PROVIDE_KEY,
+  STORYBOOK_TEST_FEATURES_PROVIDE_KEY,
   STORYBOOK_TEST_INITIAL_GLOBALS_PROVIDE_KEY,
   STORYBOOK_TEST_PROVIDE_KEY,
 } from './constants.ts';
 
 declare module 'vitest' {
   interface ProvidedContext {
+    [STORYBOOK_TEST_FEATURES_PROVIDE_KEY]: StorybookConfigRaw['features'];
     [STORYBOOK_TEST_PROVIDE_KEY]: Record<string, unknown>;
     [STORYBOOK_CORE_GHOST_STORIES_PROVIDE_KEY]: boolean;
     [STORYBOOK_CORE_RENDER_ANALYSIS_PROVIDE_KEY]: boolean;
diff --git a/yarn.lock b/yarn.lock
index 8d472c9e6f65..10e79bc366b5 100644
--- a/yarn.lock
+++ b/yarn.lock
@@ -11168,11 +11168,11 @@ __metadata:
     typescript: "npm:^6.0.3"
     vitest: "npm:^4.1.6"
   peerDependencies:
-    "@vitest/browser": ^3.0.0 || ^4.0.0
-    "@vitest/browser-playwright": ^4.0.0
-    "@vitest/runner": ^3.0.0 || ^4.0.0
+    "@vitest/browser": ^3.0.0 || ^4.0.0 || ^5.0.0
+    "@vitest/browser-playwright": ^4.0.0 || ^5.0.0
+    "@vitest/runner": ^3.0.0 || ^4.0.0 || ^5.0.0
     storybook: "workspace:^"
-    vitest: ^3.0.0 || ^4.0.0
+    vitest: ^3.0.0 || ^4.0.0 || ^5.0.0
   peerDependenciesMeta:
     "@vitest/browser":
       optional: true