diff --git a/code/builders/builder-vite/src/plugins/storybook-optimize-deps-plugin.test.ts b/code/builders/builder-vite/src/plugins/storybook-optimize-deps-plugin.test.ts
index f42d94a5fe12..b9f1062943ba 100644
--- a/code/builders/builder-vite/src/plugins/storybook-optimize-deps-plugin.test.ts
+++ b/code/builders/builder-vite/src/plugins/storybook-optimize-deps-plugin.test.ts
@@ -1,6 +1,6 @@
 import { describe, expect, it } from 'vitest';
 
-import { escapeGlobPath } from './storybook-optimize-deps-plugin';
+import { escapeGlobPath, getMockRedirectIncludeEntries } from './storybook-optimize-deps-plugin';
 
 describe('escapeGlobPath', () => {
   it('should not modify a plain path without special characters', () => {
@@ -42,3 +42,35 @@ describe('escapeGlobPath', () => {
     );
   });
 });
+
+describe('getMockRedirectIncludeEntries', () => {
+  it('should include only manual mock redirect paths', () => {
+    expect(
+      getMockRedirectIncludeEntries([
+        { redirectPath: '/project/src/lib/__mocks__/db.ts' },
+        { redirectPath: null },
+      ])
+    ).toEqual(['/project/src/lib/__mocks__/db.ts']);
+  });
+
+  it('should escape special glob characters in redirect paths', () => {
+    expect(
+      getMockRedirectIncludeEntries([
+        { redirectPath: '/project/src/(group)/__mocks__/db.ts' },
+        { redirectPath: '/project/src/[id]/__mocks__/db.ts' },
+      ])
+    ).toEqual([
+      '/project/src/\\(group\\)/__mocks__/db.ts',
+      '/project/src/\\[id\\]/__mocks__/db.ts',
+    ]);
+  });
+
+  it('should dedupe redirect paths', () => {
+    expect(
+      getMockRedirectIncludeEntries([
+        { redirectPath: '/project/src/lib/__mocks__/db.ts' },
+        { redirectPath: '/project/src/lib/__mocks__/db.ts' },
+      ])
+    ).toEqual(['/project/src/lib/__mocks__/db.ts']);
+  });
+});
diff --git a/code/builders/builder-vite/src/plugins/storybook-optimize-deps-plugin.ts b/code/builders/builder-vite/src/plugins/storybook-optimize-deps-plugin.ts
index e75ab293dff1..cf3e2e8cc3f0 100644
--- a/code/builders/builder-vite/src/plugins/storybook-optimize-deps-plugin.ts
+++ b/code/builders/builder-vite/src/plugins/storybook-optimize-deps-plugin.ts
@@ -1,5 +1,6 @@
 import { loadPreviewOrConfigFile } from 'storybook/internal/common';
 import type { StoryIndexGenerator } from 'storybook/internal/core-server';
+import { babelParser, extractMockCalls, findMockRedirect } from 'storybook/internal/mocking-utils';
 import type { Options, PreviewAnnotation, StoryIndex } from 'storybook/internal/types';
 
 import { resolve } from 'pathe';
@@ -18,6 +19,20 @@ export function escapeGlobPath(filePath: string): string {
   return filePath.replace(/[()[\]{}!*?|+@]/g, '\\$&');
 }
 
+/** Converts extracted sb.mock calls into optimizeDeps include entries for manual **mocks** files. */
+export function getMockRedirectIncludeEntries(
+  mockCalls: Array<{ redirectPath: string | null }>
+): string[] {
+  return Array.from(
+    new Set(
+      mockCalls
+        .map((mockCall) => mockCall.redirectPath)
+        .filter((redirectPath): redirectPath is string => redirectPath !== null)
+        .map(escapeGlobPath)
+    )
+  );
+}
+
 /** A Vite plugin that configures dependency optimization for Storybook's dev server. */
 export function storybookOptimizeDepsPlugin(options: Options): Plugin {
   return {
@@ -41,6 +56,22 @@ export function storybookOptimizeDepsPlugin(options: Options): Plugin {
       // Include the user's preview file and all addon/framework/renderer preview annotations
       // as optimizer entries so Vite can discover all transitive CJS dependencies automatically.
       const previewOrConfigFile = loadPreviewOrConfigFile({ configDir: options.configDir });
+
+      const mockRedirectIncludeEntries = previewOrConfigFile
+        ? getMockRedirectIncludeEntries(
+            extractMockCalls(
+              {
+                previewConfigPath: previewOrConfigFile,
+                coreOptions: { disableTelemetry: true },
+                configDir: options.configDir,
+              },
+              babelParser,
+              projectRoot,
+              findMockRedirect
+            )
+          )
+        : [];
+
       const previewAnnotationEntries = [...previewAnnotations, previewOrConfigFile]
         .filter((path): path is PreviewAnnotation => path !== undefined)
         .map((path) => processPreviewAnnotation(path, projectRoot));
@@ -56,6 +87,7 @@ export function storybookOptimizeDepsPlugin(options: Options): Plugin {
             ...(typeof config.optimizeDeps?.entries === 'string'
               ? [config.optimizeDeps.entries]
               : (config.optimizeDeps?.entries ?? [])),
+            ...mockRedirectIncludeEntries,
             ...getUniqueImportPaths(index).map(escapeGlobPath),
             ...previewAnnotationEntries.map(escapeGlobPath),
           ],