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config.yaml
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references:
basepath: "path_to_references_data"
provider: "reference_provider"
release: "reference_release"
genome_fasta: "_genome_fasta"
mirna_ref: "../data/ref/miRNA/miRBase_main_mature.fasta"
mirna_ref_precursors: "../data/ref/miRNA/miRBase_main_precursors.fasta"
mirna_other: "../data/ref/miRNA/miRBase_other_mature.fasta"
htseq_gff: "../data/ref/mirbase/file.gff3"
rules:
trim_galore_se:
params: "--small_rna --dont_gzip"
multiqc:
arguments: "--force "
mirdeep2_alignment:
params: "-e -h -j -l 18 -m -v"
mirdeep2_identification:
params: "-t hsa -P -b 0 -v"
fastq_screen:
params: "../data/fastq_screen.config"
bowtie_mapping:
params: "-v 2 -m 10 -a"
basename: "ucsc_hg19 " ## to edit
mir_trace:
params: "-s hsa"
htseq:
params: "-f bam -r pos -t miRNA -i Name"
### common codes: Homo sapiens= hsa; Mus musculus=mmu; Rattus norvegicus=rno
wrappers:
fastqc: "fastqc_wrapper"
trim_galore_se: "trim_galore_se_wrapper"
samples: samples.tsv
units: units.tsv
reheader: reheader.tsv
internal_sid: "LIMS"