diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 850ecd409b5c..40d68b4ad9c7 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -87,6 +87,12 @@ repos:
         language: system
         files: ^\.github/workflows/.*\.yml$
         pass_filenames: false
+      - id: check-registered-tests
+        name: check registered tests have CI registry
+        entry: python3 scripts/ci/check_registered_tests.py
+        language: system
+        files: ^test/registered/.*\.py$
+        pass_filenames: false
   - repo: https://github.com/lycheeverse/lychee.git
     rev: lychee-v0.22.0
     hooks:
diff --git a/scripts/ci/check_registered_tests.py b/scripts/ci/check_registered_tests.py
new file mode 100755
index 000000000000..28459a4b848b
--- /dev/null
+++ b/scripts/ci/check_registered_tests.py
@@ -0,0 +1,56 @@
+#!/usr/bin/env python3
+"""
+Pre-commit hook: validate that all Python test files under test/registered/
+contain a CI registry call (register_cuda_ci, register_amd_ci, etc.).
+
+Reuses ut_parse_one_file() from ci_register.py (AST-based parsing)
+to match the same logic used by run_suite.py's collect_tests().
+"""
+
+import glob
+import importlib.util
+import os
+import sys
+
+
+def main() -> int:
+    # Import ci_register directly to avoid pulling in all of sglang
+    spec = importlib.util.spec_from_file_location(
+        "ci_register",
+        os.path.join("python", "sglang", "test", "ci", "ci_register.py"),
+    )
+    ci_register = importlib.util.module_from_spec(spec)
+    spec.loader.exec_module(ci_register)
+
+    # Same filter as run_suite.py: skip conftest.py and __init__.py
+    files = sorted(
+        f
+        for f in glob.glob("test/registered/**/*.py", recursive=True)
+        if not f.endswith("/conftest.py") and not f.endswith("/__init__.py")
+    )
+    if not files:
+        return 0
+
+    errors = []
+    for f in files:
+        try:
+            registries = ci_register.ut_parse_one_file(f)
+            if len(registries) == 0:
+                errors.append(f)
+        except Exception:
+            # Skip files that can't be parsed (syntax errors, etc.)
+            pass
+
+    if errors:
+        print("ERROR: Files in test/registered/ missing CI registry call:")
+        print("  Move manual-only tests to test/manual/.\n")
+        for f in errors:
+            print(f"  {f}")
+        print()
+        return 1
+
+    return 0
+
+
+if __name__ == "__main__":
+    sys.exit(main())