integrate two separate datasets

[salvatore92]

Hi,

I want to integrate two datasets using seurat, So I found this tutorial https://satijalab.org/seurat/articles/integration_rpca.html
However there they are integrating over one dataset and split it into a list if I read that correctly. However what I want to do is integrating two separate datasets. But the function FindIntegrationAnchors requires to pass one list. Does anyone have a suggestion on how to create one list from two seurat objects?

[wudustan]

If you have two seurat objects eg: seuratA and seuratB

seurat.list <- list(seuratA, seurat)

However, the vignette you linked is out of date. Seurat v5 has implemented layers which holds multiple datasets as separate matrices inside one Seurat object. See the vignette https://satijalab.org/seurat/articles/seurat5_integration