diff --git a/qiskit_experiments/library/characterization/t1.py b/qiskit_experiments/library/characterization/t1.py
index 850305382c..62f593e32c 100644
--- a/qiskit_experiments/library/characterization/t1.py
+++ b/qiskit_experiments/library/characterization/t1.py
@@ -100,7 +100,7 @@ def _set_backend(self, backend: Backend):
         if not self._backend.configuration().simulator and not isinstance(backend, FakeBackend):
             timing_constraints = getattr(self.transpile_options, "timing_constraints", {})
             if "acquire_alignment" not in timing_constraints:
-                timing_constraints["aquire_aligment"] = 16
+                timing_constraints["acquire_alignment"] = 16
             scheduling_method = getattr(self.transpile_options, "scheduling_method", "alap")
             self.set_transpile_options(
                 timing_constraints=timing_constraints, scheduling_method=scheduling_method
diff --git a/qiskit_experiments/library/characterization/t2ramsey.py b/qiskit_experiments/library/characterization/t2ramsey.py
index b5791738f2..c1876801ea 100644
--- a/qiskit_experiments/library/characterization/t2ramsey.py
+++ b/qiskit_experiments/library/characterization/t2ramsey.py
@@ -111,7 +111,7 @@ def _set_backend(self, backend: Backend):
         if not self._backend.configuration().simulator and not isinstance(backend, FakeBackend):
             timing_constraints = getattr(self.transpile_options, "timing_constraints", {})
             if "acquire_alignment" not in timing_constraints:
-                timing_constraints["aquire_aligment"] = 16
+                timing_constraints["acquire_alignment"] = 16
             scheduling_method = getattr(self.transpile_options, "scheduling_method", "alap")
             self.set_transpile_options(
                 timing_constraints=timing_constraints, scheduling_method=scheduling_method