diff --git a/changelog/11028.bugfix.rst b/changelog/11028.bugfix.rst
new file mode 100644
index 00000000000..9efc04ba69f
--- /dev/null
+++ b/changelog/11028.bugfix.rst
@@ -0,0 +1 @@
+Fixed bug in assertion rewriting where a variable assigned with the walrus operator could not be used later in a function call.
diff --git a/src/_pytest/assertion/rewrite.py b/src/_pytest/assertion/rewrite.py
index 8b182347052..ab8169da2ef 100644
--- a/src/_pytest/assertion/rewrite.py
+++ b/src/_pytest/assertion/rewrite.py
@@ -996,7 +996,9 @@ def visit_BoolOp(self, boolop: ast.BoolOp) -> Tuple[ast.Name, str]:
                     ]
                 ):
                     pytest_temp = self.variable()
-                    self.variables_overwrite[v.left.target.id] = pytest_temp
+                    self.variables_overwrite[
+                        v.left.target.id
+                    ] = v.left  # type:ignore[assignment]
                     v.left.target.id = pytest_temp
             self.push_format_context()
             res, expl = self.visit(v)
@@ -1037,10 +1039,19 @@ def visit_Call(self, call: ast.Call) -> Tuple[ast.Name, str]:
         new_args = []
         new_kwargs = []
         for arg in call.args:
+            if isinstance(arg, ast.Name) and arg.id in self.variables_overwrite:
+                arg = self.variables_overwrite[arg.id]  # type:ignore[assignment]
             res, expl = self.visit(arg)
             arg_expls.append(expl)
             new_args.append(res)
         for keyword in call.keywords:
+            if (
+                isinstance(keyword.value, ast.Name)
+                and keyword.value.id in self.variables_overwrite
+            ):
+                keyword.value = self.variables_overwrite[
+                    keyword.value.id
+                ]  # type:ignore[assignment]
             res, expl = self.visit(keyword.value)
             new_kwargs.append(ast.keyword(keyword.arg, res))
             if keyword.arg:
@@ -1075,7 +1086,13 @@ def visit_Compare(self, comp: ast.Compare) -> Tuple[ast.expr, str]:
         self.push_format_context()
         # We first check if we have overwritten a variable in the previous assert
         if isinstance(comp.left, ast.Name) and comp.left.id in self.variables_overwrite:
-            comp.left.id = self.variables_overwrite[comp.left.id]
+            comp.left = self.variables_overwrite[
+                comp.left.id
+            ]  # type:ignore[assignment]
+        if isinstance(comp.left, namedExpr):
+            self.variables_overwrite[
+                comp.left.target.id
+            ] = comp.left  # type:ignore[assignment]
         left_res, left_expl = self.visit(comp.left)
         if isinstance(comp.left, (ast.Compare, ast.BoolOp)):
             left_expl = f"({left_expl})"
@@ -1093,7 +1110,9 @@ def visit_Compare(self, comp: ast.Compare) -> Tuple[ast.expr, str]:
                 and next_operand.target.id == left_res.id
             ):
                 next_operand.target.id = self.variable()
-                self.variables_overwrite[left_res.id] = next_operand.target.id
+                self.variables_overwrite[
+                    left_res.id
+                ] = next_operand  # type:ignore[assignment]
             next_res, next_expl = self.visit(next_operand)
             if isinstance(next_operand, (ast.Compare, ast.BoolOp)):
                 next_expl = f"({next_expl})"
diff --git a/testing/test_assertrewrite.py b/testing/test_assertrewrite.py
index 8d944140307..245241af2b2 100644
--- a/testing/test_assertrewrite.py
+++ b/testing/test_assertrewrite.py
@@ -1436,6 +1436,96 @@ def test_walrus_operator_not_override_value():
         assert result.ret == 0
 
 
+@pytest.mark.skipif(
+    sys.version_info < (3, 8), reason="walrus operator not available in py<38"
+)
+class TestIssue11028:
+    def test_assertion_walrus_operator_in_operand(self, pytester: Pytester) -> None:
+        pytester.makepyfile(
+            """
+            def test_in_string():
+              assert (obj := "foo") in obj
+        """
+        )
+        result = pytester.runpytest()
+        assert result.ret == 0
+
+    def test_assertion_walrus_operator_in_operand_json_dumps(
+        self, pytester: Pytester
+    ) -> None:
+        pytester.makepyfile(
+            """
+            import json
+
+            def test_json_encoder():
+                assert (obj := "foo") in json.dumps(obj)
+        """
+        )
+        result = pytester.runpytest()
+        assert result.ret == 0
+
+    def test_assertion_walrus_operator_equals_operand_function(
+        self, pytester: Pytester
+    ) -> None:
+        pytester.makepyfile(
+            """
+            def f(a):
+                return a
+
+            def test_call_other_function_arg():
+              assert (obj := "foo") == f(obj)
+        """
+        )
+        result = pytester.runpytest()
+        assert result.ret == 0
+
+    def test_assertion_walrus_operator_equals_operand_function_keyword_arg(
+        self, pytester: Pytester
+    ) -> None:
+        pytester.makepyfile(
+            """
+            def f(a='test'):
+                return a
+
+            def test_call_other_function_k_arg():
+              assert (obj := "foo") == f(a=obj)
+        """
+        )
+        result = pytester.runpytest()
+        assert result.ret == 0
+
+    def test_assertion_walrus_operator_equals_operand_function_arg_as_function(
+        self, pytester: Pytester
+    ) -> None:
+        pytester.makepyfile(
+            """
+            def f(a='test'):
+                return a
+
+            def test_function_of_function():
+              assert (obj := "foo") == f(f(obj))
+        """
+        )
+        result = pytester.runpytest()
+        assert result.ret == 0
+
+    def test_assertion_walrus_operator_gt_operand_function(
+        self, pytester: Pytester
+    ) -> None:
+        pytester.makepyfile(
+            """
+            def add_one(a):
+                return a + 1
+
+            def test_gt():
+              assert (obj := 4) > add_one(obj)
+        """
+        )
+        result = pytester.runpytest()
+        assert result.ret == 1
+        result.stdout.fnmatch_lines(["*assert 4 > 5", "*where 5 = add_one(4)"])
+
+
 @pytest.mark.skipif(
     sys.maxsize <= (2**31 - 1), reason="Causes OverflowError on 32bit systems"
 )