diff --git a/docs/changelog/3160.bugfix.rst b/docs/changelog/3160.bugfix.rst
new file mode 100644
index 000000000..00ed6176d
--- /dev/null
+++ b/docs/changelog/3160.bugfix.rst
@@ -0,0 +1 @@
+Handle CYGWIN/MSYS/MINGW path conversions in fish activation script - by user::`LuNoX`.
diff --git a/src/virtualenv/activation/fish/activate.fish b/src/virtualenv/activation/fish/activate.fish
index 33a8c7fc4..58a12d761 100644
--- a/src/virtualenv/activation/fish/activate.fish
+++ b/src/virtualenv/activation/fish/activate.fish
@@ -61,6 +61,9 @@ end
 deactivate nondestructive
 
 set -gx VIRTUAL_ENV __VIRTUAL_ENV__
+if string match -qr 'CYGWIN|MSYS|MINGW' (uname)
+    set -gx VIRTUAL_ENV (cygpath -u $VIRTUAL_ENV)
+end
 
 set -gx _OLD_PKG_CONFIG_PATH "$PKG_CONFIG_PATH"
 set -gx PKG_CONFIG_PATH "$VIRTUAL_ENV/lib/pkgconfig:$PKG_CONFIG_PATH"