diff --git a/doc/whatsnew/fragments/8463.internal b/doc/whatsnew/fragments/8463.internal
new file mode 100644
index 0000000000..de5df25200
--- /dev/null
+++ b/doc/whatsnew/fragments/8463.internal
@@ -0,0 +1,3 @@
+Following a deprecation period, ``Pylinter.check`` now only work with sequences of strings, not strings.
+
+Refs #8463
diff --git a/pylint/lint/pylinter.py b/pylint/lint/pylinter.py
index 90f7cd75cf..cde21627de 100644
--- a/pylint/lint/pylinter.py
+++ b/pylint/lint/pylinter.py
@@ -637,23 +637,12 @@ def _discover_files(self, files_or_modules: Sequence[str]) -> Iterator[str]:
             else:
                 yield something
 
-    def check(self, files_or_modules: Sequence[str] | str) -> None:
+    def check(self, files_or_modules: Sequence[str]) -> None:
         """Main checking entry: check a list of files or modules from their name.
 
         files_or_modules is either a string or list of strings presenting modules to check.
         """
-        # 1) Initialize
         self.initialize()
-
-        # 2) Gather all files
-        if not isinstance(files_or_modules, (list, tuple)):
-            # TODO: 3.0: Remove deprecated typing and update docstring
-            warnings.warn(
-                "In pylint 3.0, the checkers check function will only accept sequence of string",
-                DeprecationWarning,
-                stacklevel=2,
-            )
-            files_or_modules = (files_or_modules,)  # type: ignore[assignment]
         if self.config.recursive:
             files_or_modules = tuple(self._discover_files(files_or_modules))
         if self.config.from_stdin:
@@ -669,7 +658,7 @@ def check(self, files_or_modules: Sequence[str] | str) -> None:
             }
         )
 
-        # TODO: Move the parallel invocation into step 5 of the checking process
+        # TODO: Move the parallel invocation into step 3 of the checking process
         if not self.config.from_stdin and self.config.jobs > 1:
             original_sys_path = sys.path[:]
             check_parallel(
@@ -681,7 +670,7 @@ def check(self, files_or_modules: Sequence[str] | str) -> None:
             sys.path = original_sys_path
             return
 
-        # 3) Get all FileItems
+        # 1) Get all FileItems
         with augmented_sys_path(extra_packages_paths):
             if self.config.from_stdin:
                 fileitems = self._get_file_descr_from_stdin(files_or_modules[0])
@@ -693,10 +682,10 @@ def check(self, files_or_modules: Sequence[str] | str) -> None:
         # The contextmanager also opens all checkers and sets up the PyLinter class
         with augmented_sys_path(extra_packages_paths):
             with self._astroid_module_checker() as check_astroid_module:
-                # 4) Get the AST for each FileItem
+                # 2) Get the AST for each FileItem
                 ast_per_fileitem = self._get_asts(fileitems, data)
 
-                # 5) Lint each ast
+                # 3) Lint each ast
                 self._lint_files(ast_per_fileitem, check_astroid_module)
 
     def _get_asts(
diff --git a/tests/lint/test_pylinter.py b/tests/lint/test_pylinter.py
index 6ccce8fca7..f7e7cf4c47 100644
--- a/tests/lint/test_pylinter.py
+++ b/tests/lint/test_pylinter.py
@@ -8,7 +8,6 @@
 from unittest import mock
 from unittest.mock import patch
 
-from _pytest.recwarn import WarningsRecorder
 from pytest import CaptureFixture
 
 from pylint.lint.pylinter import PyLinter
@@ -34,12 +33,6 @@ def test_crash_in_file(
     assert any(m.symbol == "fatal" for m in linter.reporter.messages)
 
 
-def test_check_deprecation(linter: PyLinter, recwarn: WarningsRecorder) -> None:
-    linter.check("myfile.py")
-    msg = recwarn.pop()
-    assert "check function will only accept sequence" in str(msg)
-
-
 def test_crash_during_linting(
     linter: PyLinter, capsys: CaptureFixture[str], tmp_path: Path
 ) -> None: