diff --git a/zkevm-circuits/src/evm_circuit/execution.rs b/zkevm-circuits/src/evm_circuit/execution.rs
index b927b4d817..dc168048a2 100644
--- a/zkevm-circuits/src/evm_circuit/execution.rs
+++ b/zkevm-circuits/src/evm_circuit/execution.rs
@@ -58,6 +58,7 @@ mod memory_copy;
 mod msize;
 mod mul_div_mod;
 mod mulmod;
+mod not;
 mod origin;
 mod pc;
 mod pop;
@@ -107,6 +108,7 @@ use memory_copy::CopyToMemoryGadget;
 use msize::MsizeGadget;
 use mul_div_mod::MulDivModGadget;
 use mulmod::MulModGadget;
+use not::NotGadget;
 use origin::OriginGadget;
 use pc::PcGadget;
 use pop::PopGadget;
@@ -185,6 +187,7 @@ pub(crate) struct ExecutionConfig<F> {
     msize_gadget: MsizeGadget<F>,
     mul_div_mod_gadget: MulDivModGadget<F>,
     mulmod_gadget: MulModGadget<F>,
+    not_gadget: NotGadget<F>,
     origin_gadget: OriginGadget<F>,
     pc_gadget: PcGadget<F>,
     pop_gadget: PopGadget<F>,
@@ -379,6 +382,7 @@ impl<F: Field> ExecutionConfig<F> {
             msize_gadget: configure_gadget!(),
             mul_div_mod_gadget: configure_gadget!(),
             mulmod_gadget: configure_gadget!(),
+            not_gadget: configure_gadget!(),
             origin_gadget: configure_gadget!(),
             pc_gadget: configure_gadget!(),
             pop_gadget: configure_gadget!(),
@@ -818,6 +822,7 @@ impl<F: Field> ExecutionConfig<F> {
             ExecutionState::MSIZE => assign_exec_step!(self.msize_gadget),
             ExecutionState::MUL_DIV_MOD => assign_exec_step!(self.mul_div_mod_gadget),
             ExecutionState::MULMOD => assign_exec_step!(self.mulmod_gadget),
+            ExecutionState::NOT => assign_exec_step!(self.not_gadget),
             ExecutionState::ORIGIN => assign_exec_step!(self.origin_gadget),
             ExecutionState::PC => assign_exec_step!(self.pc_gadget),
             ExecutionState::POP => assign_exec_step!(self.pop_gadget),
diff --git a/zkevm-circuits/src/evm_circuit/execution/not.rs b/zkevm-circuits/src/evm_circuit/execution/not.rs
new file mode 100644
index 0000000000..b0b245ab2e
--- /dev/null
+++ b/zkevm-circuits/src/evm_circuit/execution/not.rs
@@ -0,0 +1,135 @@
+use crate::{
+    evm_circuit::{
+        execution::ExecutionGadget,
+        step::ExecutionState,
+        table::{FixedTableTag, Lookup},
+        util::{
+            common_gadget::SameContextGadget,
+            constraint_builder::{ConstraintBuilder, StepStateTransition, Transition::Delta},
+            CachedRegion, Word,
+        },
+        witness::{Block, Call, ExecStep, Transaction},
+    },
+    util::Expr,
+};
+use eth_types::evm_types::OpcodeId;
+use eth_types::{Field, ToLittleEndian};
+use halo2_proofs::plonk::Error;
+
+#[derive(Clone, Debug)]
+pub(crate) struct NotGadget<F> {
+    same_context: SameContextGadget<F>,
+    input: Word<F>,
+    output: Word<F>,
+}
+
+impl<F: Field> ExecutionGadget<F> for NotGadget<F> {
+    const NAME: &'static str = "NOT";
+
+    const EXECUTION_STATE: ExecutionState = ExecutionState::NOT;
+
+    fn configure(cb: &mut ConstraintBuilder<F>) -> Self {
+        let opcode = cb.query_cell();
+
+        let input = cb.query_word();
+        let output = cb.query_word();
+
+        cb.stack_pop(input.expr());
+        cb.stack_push(output.expr());
+
+        for (i, o) in input.cells.iter().zip(output.cells.iter()) {
+            cb.add_lookup(
+                "input XOR output is all 1's",
+                Lookup::Fixed {
+                    tag: FixedTableTag::BitwiseXor.expr(),
+                    values: [i.expr(), o.expr(), 255.expr()],
+                },
+            );
+        }
+
+        // State transition
+        let step_state_transition = StepStateTransition {
+            rw_counter: Delta(2.expr()),
+            program_counter: Delta(1.expr()),
+            stack_pointer: Delta(0.expr()),
+            gas_left: Delta(-OpcodeId::NOT.constant_gas_cost().expr()),
+            ..Default::default()
+        };
+        let same_context = SameContextGadget::construct(cb, opcode, step_state_transition);
+
+        Self {
+            same_context,
+            input,
+            output,
+        }
+    }
+
+    fn assign_exec_step(
+        &self,
+        region: &mut CachedRegion<'_, '_, F>,
+        offset: usize,
+        block: &Block<F>,
+        _: &Transaction,
+        _: &Call,
+        step: &ExecStep,
+    ) -> Result<(), Error> {
+        self.same_context.assign_exec_step(region, offset, step)?;
+
+        let [input, output] =
+            [step.rw_indices[0], step.rw_indices[1]].map(|idx| block.rws[idx].stack_value());
+        self.input
+            .assign(region, offset, Some(input.to_le_bytes()))?;
+        self.output
+            .assign(region, offset, Some(output.to_le_bytes()))?;
+
+        Ok(())
+    }
+}
+
+#[cfg(test)]
+mod test {
+    use crate::{
+        evm_circuit::test::rand_word,
+        test_util::{get_fixed_table, run_test_circuits, BytecodeTestConfig, FixedTableConfig},
+    };
+    use eth_types::{bytecode, Word};
+    use mock::TestContext;
+
+    fn test_ok(a: Word) {
+        let bytecode = bytecode! {
+            PUSH32(a)
+            NOT
+            STOP
+        };
+        let test_config = BytecodeTestConfig {
+            evm_circuit_lookup_tags: get_fixed_table(FixedTableConfig::Complete),
+            ..Default::default()
+        };
+
+        assert_eq!(
+            run_test_circuits(
+                TestContext::<1, 1>::simple_ctx_with_bytecode(bytecode).unwrap(),
+                Some(test_config)
+            ),
+            Ok(())
+        );
+    }
+
+    #[test]
+    fn not_gadget_simple() {
+        test_ok(0.into());
+        test_ok(1.into());
+        test_ok(255.into());
+        test_ok(256.into());
+        test_ok(Word::MAX);
+    }
+
+    #[test]
+    fn not_gadget_rand() {
+        let a = rand_word();
+        // the debug statement is useful for random tests so in case it fails, the
+        // failing example shows up in the logs.
+        dbg!(a);
+        test_ok(a);
+    }
+}
diff --git a/zkevm-circuits/src/evm_circuit/witness.rs b/zkevm-circuits/src/evm_circuit/witness.rs
index 083a68fe05..3896340559 100644
--- a/zkevm-circuits/src/evm_circuit/witness.rs
+++ b/zkevm-circuits/src/evm_circuit/witness.rs
@@ -1207,6 +1207,7 @@ impl From<&circuit_input_builder::ExecStep> for ExecutionState {
                     OpcodeId::AND => ExecutionState::BITWISE,
                     OpcodeId::XOR => ExecutionState::BITWISE,
                     OpcodeId::OR => ExecutionState::BITWISE,
+                    OpcodeId::NOT => ExecutionState::NOT,
                     OpcodeId::POP => ExecutionState::POP,
                     OpcodeId::PUSH32 => ExecutionState::PUSH,
                     OpcodeId::BYTE => ExecutionState::BYTE,