diff --git a/.changeset/clever-cats-carry.md b/.changeset/clever-cats-carry.md
new file mode 100644
index 0000000000..a9cb6bdeed
--- /dev/null
+++ b/.changeset/clever-cats-carry.md
@@ -0,0 +1,5 @@
+---
+"@primer/view-components": patch
+---
+
+Make Retry button focusable in loading_failure_story
diff --git a/previews/primer/alpha/tree_view_preview/loading_failure.html.erb b/previews/primer/alpha/tree_view_preview/loading_failure.html.erb
index d324e8e703..7810d52b3d 100644
--- a/previews/primer/alpha/tree_view_preview/loading_failure.html.erb
+++ b/previews/primer/alpha/tree_view_preview/loading_failure.html.erb
@@ -29,8 +29,60 @@
     const includeFragment = subject.querySelector('tree-view-include-fragment')
     if (!includeFragment) return
 
-    includeFragment.addEventListener('loadend', (event) => {
+    function retryButton() {
+      return subject.querySelector("[data-target='tree-view-sub-tree-node.retryButton']")
+    }
+
+    function focusTreeItem() {
+      const treeItem = subject.querySelector("[role='treeitem']")
+      if (treeItem) treeItem.focus()
+    }
+
+    function makeRetryTabbable() {
+      const retry = retryButton()
+      if (!retry) return
+      // Remove tabindex="-1" so the button can be reached by keyboard.
+      retry.removeAttribute('tabindex')
+    }
+
+    function addRetryFocusHandler() {
+      const retry = retryButton()
+      if (!retry) return
+
+      if (retry.dataset.focusHandlerAttached === "true") return
+      retry.dataset.focusHandlerAttached = "true"
+
+      retry.addEventListener('click', () => {
+        focusTreeItem()
+      })
+    }
+
+    // When the failure UI loads, ensure Retry is tabbable
+    includeFragment.addEventListener('loadend', (_event) => {
+      makeRetryTabbable()
+      addRetryFocusHandler()
       subject.setAttribute('data-ready', 'true')
     })
+
+    // If include-fragment swaps in new markup on re-render, re-apply tabindex removal.
+    includeFragment.addEventListener('include-fragment-replaced', () => {
+      makeRetryTabbable()
+      addRetryFocusHandler()
+    })
+
+    // If Retry exists and the user presses Tab while focused inside this TreeView,
+    // redirect focus to Retry so it is reachable without requiring a mouse click.
+    subject.addEventListener('keydown', (event) => {
+      if (event.key !== 'Tab' || event.shiftKey) return
+      const active = document.activeElement
+      if (!(active instanceof HTMLElement)) return
+      if (!subject.contains(active)) return
+      if (active.getAttribute('role') !== 'treeitem') return
+      const retry = retryButton()
+      if (!retry) return
+      makeRetryTabbable()
+      event.preventDefault()
+      retry.focus()
+    })
   })
 </script>