diff --git a/.changeset/yellow-shoes-glow.md b/.changeset/yellow-shoes-glow.md
new file mode 100644
index 00000000000..3a39d531690
--- /dev/null
+++ b/.changeset/yellow-shoes-glow.md
@@ -0,0 +1,5 @@
+---
+'@primer/react': patch
+---
+
+Trigger onSelect when TreeView items are middle clicked
diff --git a/src/TreeView/TreeView.test.tsx b/src/TreeView/TreeView.test.tsx
index 03c77138768..5e443db9edc 100644
--- a/src/TreeView/TreeView.test.tsx
+++ b/src/TreeView/TreeView.test.tsx
@@ -778,6 +778,13 @@ describe('Keyboard interactions', () => {
 
       // onSelect should have been called
       expect(onSelect).toHaveBeenCalledTimes(1)
+
+      onSelect.mockClear()
+      // Press middle click
+      fireEvent.click(document.activeElement?.firstChild || document.body, {button: 1})
+
+      // onSelect should have been called
+      expect(onSelect).toHaveBeenCalledTimes(1)
     })
 
     it('toggles expanded state if no onSelect function is provided', () => {
diff --git a/src/TreeView/TreeView.tsx b/src/TreeView/TreeView.tsx
index 21f2660f3b2..d623bab37d9 100644
--- a/src/TreeView/TreeView.tsx
+++ b/src/TreeView/TreeView.tsx
@@ -429,6 +429,11 @@ const Item = React.forwardRef<HTMLElement, TreeViewItemProps>(
                 toggle(event)
               }
             }}
+            onAuxClick={event => {
+              if (onSelect && event.button === 1) {
+                onSelect(event)
+              }
+            }}
           >
             <div style={{gridArea: 'spacer', display: 'flex'}}>
               <LevelIndicatorLines level={level} />