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index.html
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<html>
<head>
<!-- Load plotly.js into the DOM -->
<!--<script src='https://cdn.plot.ly/plotly-2.12.1.min.js'></script> -->
<script src='./plotly-2.12.1.min.js'></script>
<!--
ChangeLog:
2022-08-04:
todo:
1.蛋白全长水平的domain regeion 区域 或者 template蛋白的二级结构注释(来自https://hub.0z.gs/YuLab-SMU/ggmsa/issues/47)
2.输入一小段蛋白序列(支持js的正则表达式)，在template中搜索和定位这段序列的位置，比如在5个不同位置都搜索到这段序列，则把这5个位置作为一个新的track data展示（相当在整个蛋白长度水平上高亮这5个位置），同时也展示这5个位置的信息作为一列（5行），每行表示一个match的位置都可以点击后使用plot_jump_to_x()函数和起始位置坐标 来跳转到对应的match区域。
-->
</head>
<body>
<p>输入fasta格式的MSA序列: <input type="button" onclick="clear_msa()" value="Clear Current sMSA" /></p>
<textarea id="msa" name="msa" rows="20" cols="100">
>UrCas13d-HA
-----------MAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNQT
QLSSKDNSNIQLGGVNEVNITFSSKHGFESGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGVKGSE--SH
DDFIGYLSTNNIYDVFIDPD-NSSLSDDKKANVRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDNRVSQAYKKR------VYHMLAIVGQIRQCVFHDKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEDNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISG-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYRNCIAH
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEK--YPYDVP
D---------YA--
>dUrCas13d-HA
-----------MAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNQT
QLSSKDNSNIQLGGVNEVNITFSSKHGFESGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGVKGSE--SH
DDFIGYLSTNNIYDVFIDPD-NSSLSDDKKANVRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDNRVSQAYKKR------VYHMLAIVGQIAQCVFADKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEDNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISG-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYANCIAA
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEK--YPYDVP
D---------YA--
>NLS-UrCas13d-NLS-HA
MSPKKKRKVEASAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNQT
QLSSKDNSNIQLGGVNEVNITFSSKHGFESGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGVKGSE--SH
DDFIGYLSTNNIYDVFIDPD-NSSLSDDKKANVRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDNRVSQAYKKR------VYHMLAIVGQIRQCVFHDKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEDNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISG-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYRNCIAH
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEKGSGPKKKR
K---------VAAA
>NLS-dUrCas13d-NLS-HA
MSPKKKRKVEASAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNQT
QLSSKDNSNIQLGGVNEVNITFSSKHGFESGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGVKGSE--SH
DDFIGYLSTNNIYDVFIDPD-NSSLSDDKKANVRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDNRVSQAYKKR------VYHMLAIVGQIAQCVFADKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEDNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISG-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYANCIAA
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEKGSGPKKKR
K---------VAAA
>P1E0Cas13d-HA
M----------------------------EREV----------KKPP-------------
-----KKSLAKAAGLKSTFVISPQEKELAMTAFGRGNDALLQKRIVDGVVRDVAGEKQQF
QVQRQDESRFRLQNSRLADRT------------VTA--DDPLHRAETPRRQPLGAGMDQL
RRKAILEQKYFGRTF-DDNIHIQLIYNILDIHKMLAVPANHIVHTLNLLGG------YGE
TDFVGMLPAGLPYD---------KLRVVKKKNGDTVDIKADIAAYAKRPQLAYLG--AAF
YDVTPGKSKRDAARGRVKREQDVYAILSLMSLLRQFCAHDSVRIWGQNTTAALYH-LQAL
PQDMKDLLDDGWRRALGGVNDHFLDTNKVNLLTLFEYYGAETKQARVALTQDFYRFVVLK
EQKNMGFSLRRLREELLKLPDAAYLTGQEYDSVRQKLYMLLDFLLCRLYAQERADRCEEL
VSALRCALSDEEKDTVYQAEAAALWQALGDTLR--------RKLLPLLKGKKLQDKDKKK
SDELGLSRDVLDGVLFRPAQQGSRANADYFCRLMHLSTWFMDGKEINTLLTTLISKLENI
DSLRSVLES--MGLAYSFVPAYAMFDH---SRYIAGQLRVVNNIARMRKPAIGAKREMYR
AAVVLLGVDSPEAAAAITDDLLQI-------DPETGKVRPRSDSARDTGLRNFIANNVVE
SRRFTYLLRYMTPEQARVLAQNEKLIAFVLSTVPDTQLERYCRTCGR-EDIT-GRPAQIR
YLTAQIMGVRYESFTDVE-----QRGRG-DNPKKERYKALIGLYLTVLYLAVKNMVNCNA
RYVIAFYCRDRDTALYQK---EVCWYDLEE---DKKSGKQRQVEDYT-------------
------ALTRYFVSQGYLNRHACGYLRSNMNGIS--------NS-----LLTAYRNAVDH
LNAIPPLGSLCRDI-------GRVDSYFALYHYAVQQYLNGRYYRKTPREQELF---AAM
AQHRTWC-------SDLVKALNTPFGYNLARYKNLSIDGLFDREGDHVVRED--GEKPAE
--------------
>NLS-P1E0Cas13d-NLS-HA
M-----------------SPKKKRKVEASEREV----------KKPP-------------
-----KKSLAKAAGLKSTFVISPQEKELAMTAFGRGNDALLQKRIVDGVVRDVAGEKQQF
QVQRQDESRFRLQNSRLADRT------------VTA--DDPLHRAETPRRQPLGAGMDQL
RRKAILEQKYFGRTF-DDNIHIQLIYNILDIHKMLAVPANHIVHTLNLLGG------YGE
TDFVGMLPAGLPYD---------KLRVVKKKNGDTVDIKADIAAYAKRPQLAYLG--AAF
YDVTPGKSKRDAARGRVKREQDVYAILSLMSLLRQFCAHDSVRIWGQNTTAALYH-LQAL
PQDMKDLLDDGWRRALGGVNDHFLDTNKVNLLTLFEYYGAETKQARVALTQDFYRFVVLK
EQKNMGFSLRRLREELLKLPDAAYLTGQEYDSVRQKLYMLLDFLLCRLYAQERADRCEEL
VSALRCALSDEEKDTVYQAEAAALWQALGDTLR--------RKLLPLLKGKKLQDKDKKK
SDELGLSRDVLDGVLFRPAQQGSRANADYFCRLMHLSTWFMDGKEINTLLTTLISKLENI
DSLRSVLES--MGLAYSFVPAYAMFDH---SRYIAGQLRVVNNIARMRKPAIGAKREMYR
AAVVLLGVDSPEAAAAITDDLLQI-------DPETGKVRPRSDSARDTGLRNFIANNVVE
SRRFTYLLRYMTPEQARVLAQNEKLIAFVLSTVPDTQLERYCRTCGR-EDIT-GRPAQIR
YLTAQIMGVRYESFTDVE-----QRGRG-DNPKKERYKALIGLYLTVLYLAVKNMVNCNA
RYVIAFYCRDRDTALYQK---EVCWYDLEE---DKKSGKQRQVEDYT-------------
------ALTRYFVSQGYLNRHACGYLRSNMNGIS--------NS-----LLTAYRNAVDH
LNAIPPLGSLCRDI-------GRVDSYFALYHYAVQQYLNGRYYRKTPREQELF---AAM
AQHRTWC-------SDLVKALNTPFGYNLARYKNLSIDGLFDREGDHVVRED--GEKPAE
G-SGPKKKRKVAAA
>RffCas13d-HA
M-------------KKKMSLREKREAEKQAKKAAYSAASKNTDSKPAEKKAETPKPAEII
SDNSRNKTAVKAAGLKSTII---SGDKLYMTSFGKGNAAVIEQKIDI-------NDYSFS
AM--KDTPSLEVDKAESKEISFSSHHPFVKNDKLTT--YNPLYGGKDNPEKP--VGRDML
GLKDKLEERYFGCTF-NDNLHIQIIYNILDIEKILAVHSANITTALDHMVD-EDDEKYLN
SDYIGYMNTINTYDVFMDPSKNSSLSPKDRKNIDNSRAKFE--KLLSTKRLGYFG--FDY
DANGKDKKKNEEIKKR------LYHLTAFAGQLRQWSFHSAGN----YPRTWLYK-LDSL
DKEYLDTLDHYFDKRFNDINDDFVTKNATNLYILKEVFPEANFKDIADL---YYDFIVIK
SHKNMGFSIKKLREKMLECDGADRIKEQDMDSVRSKLYKLIDFCIF-KYYHEFPELSEKN
VDILRAAVSDTKKDNLYSDEAARLWSIFKEKFL-GFCDKI---VVW-VTGEHEKDITSVI
DK----------------DAYRNRSNVSYFSKLMYAMCFFLDGKEINDLLTTLINKFDNI
ANQIKTAKE--LGINTAFVKNYDFFNH---SEKYVDELNIVKNIARMKKPSSNAKKAMYH
DALTILGIPEDMDE-KALDEELDL-ILEKKTDPVTGKPLKGKN-----PLRNFIANNVIE
NSRFIYLIKFCNPENVRKIVNNTKVTEFVLKRIPDAQIERYYKSCTD-SEMNPPTEKKIT
ELAGKLKDMNFGNFRNVR-----QSAK--ENMEKERFKAVIGLYLTVVYRVVKNLVDVNS
RYIMAFHSLERDSQLY--------NVSVDN---DYLALTDTLVKEGD-------------
------NSRSRYLAG---NKRLRDCVKQDIDNAKKWFVSDKYNS-----IT-KYRNNVAH
LTAVRNCAEFIGDI-------TKIDSYFALYHYLIQRQLAKGLDHERSGFDRNYPQYAPL
FKWHTYV-------KDVVKALNAPFGYNIPRFKNLSIDALFDR--NEIKKND--GEKKSD
D-------------
>NLS-RffCas13d-NLS-HA
MSPKKKRKV--EASKKKMSLREKREAEKQAKKAAYSAASKNTDSKPAEKKAETPKPAEII
SDNSRNKTAVKAAGLKSTII---SGDKLYMTSFGKGNAAVIEQKIDI-------NDYSFS
AM--KDTPSLEVDKAESKEISFSSHHPFVKNDKLTT--YNPLYGGKDNPEKP--VGRDML
GLKDKLEERYFGCTF-NDNLHIQIIYNILDIEKILAVHSANITTALDHMVD-EDDEKYLN
SDYIGYMNTINTYDVFMDPSKNSSLSPKDRKNIDNSRAKFE--KLLSTKRLGYFG--FDY
DANGKDKKKNEEIKKR------LYHLTAFAGQLRQWSFHSAGN----YPRTWLYK-LDSL
DKEYLDTLDHYFDKRFNDINDDFVTKNATNLYILKEVFPEANFKDIADL---YYDFIVIK
SHKNMGFSIKKLREKMLECDGADRIKEQDMDSVRSKLYKLIDFCIF-KYYHEFPELSEKN
VDILRAAVSDTKKDNLYSDEAARLWSIFKEKFL-GFCDKI---VVW-VTGEHEKDITSVI
DK----------------DAYRNRSNVSYFSKLMYAMCFFLDGKEINDLLTTLINKFDNI
ANQIKTAKE--LGINTAFVKNYDFFNH---SEKYVDELNIVKNIARMKKPSSNAKKAMYH
DALTILGIPEDMDE-KALDEELDL-ILEKKTDPVTGKPLKGKN-----PLRNFIANNVIE
NSRFIYLIKFCNPENVRKIVNNTKVTEFVLKRIPDAQIERYYKSCTD-SEMNPPTEKKIT
ELAGKLKDMNFGNFRNVR-----QSAK--ENMEKERFKAVIGLYLTVVYRVVKNLVDVNS
RYIMAFHSLERDSQLY--------NVSVDN---DYLALTDTLVKEGD-------------
------NSRSRYLAG---NKRLRDCVKQDIDNAKKWFVSDKYNS-----IT-KYRNNVAH
LTAVRNCAEFIGDI-------TKIDSYFALYHYLIQRQLAKGLDHERSGFDRNYPQYAPL
FKWHTYV-------KDVVKALNAPFGYNIPRFKNLSIDALFDR--NEIKKND--GEKKSD
DGSGPKKKRKVAAA
>UrCas13d
-----------MAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PVAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNKT
QLSSKDNSNIELGDVNEVNITFSSKHGFGSGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGIKDSE--SY
DDFMGYLSARNTYEVFTHPD-KSNLSDKVKGNIKKSLSKFN--DLLKTKRLGYFG--LE-
EPKTKDTRASEAYKKR------VYHMLAIVGQIRQCVFHDKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEGNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLEEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDVAAGEAL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----NITDDRISE-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLEAKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCDDRN----------
------ESSNLFLKK---NKRLRKCVEVDINNAD--------SS-----MTRKYRNCIAH
LTVVRELKEYIGDI-------RTVDSYFSIYHYVMQRCITKRGDDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEK--------
--------------
>cas13_RBI_RS12820.cas13d_WP_041337480.1
-----------MAKKNKMKPRELREAQKKARQL--KAAEINNNAVPA--IAAMPAAEAAA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNKT
QLSSKDNSNIELCDVGKVNITFSSRRGFESGVEINT--SNPTHR--SGESSS--VRGDML
GLKSELEKRFFGKNF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLG-EGDE--SN
YDFMGYLSTFNTYKVFTNPN-GSTLSDDKKENIRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDTRASEAYKKR------VYHMLAIVGQIRQCVFHDKSG----AKRFDLYSFINNI
DPEYRETLDYLVDERFDSINKGFIQGNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISE-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYRNCIAH
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEK--------
--------------
</textarea>
<input type="button" onclick="plot_msa()" value="Plot!" />
<br><br>
<input type="button" onclick="plot_jump_to_x(1)" value="(msa mastrix coordinate)jump to" /> <input type="number" id="jump_pos_start" /><text>bp</text>
<br><br>
<input type="button" onclick="plot_jump_to_x(2)" value="(first sequence as template coordinate)jump to" /> <input type="number" id="jump_pos_start2" /><text>bp</text>
<br><br>
<input type="button" onclick="plot_msa()" value="set margin of sequence name region" /> <input type="number" id="seq_name_left_margin" value="200" /><text>px</text>
<div id='myDiv'><!-- Plotly chart will be drawn inside this DIV --></div>
<script>
// open this by https://codepen.io/pen
// https://plotly.com/javascript/colorscales/
// colorschemes from ggmsa at http://yulab-smu.top/ggmsa/articles/guides/Color_schemes_And_Font_Families.html
window.data = [];
window.layout = {};
function plot_jump_to_x(e){
var xstart = 0;
var xend=0;
var xrange = window.layout.xaxis.range;
xrange_unit = xrange[1]-xrange[0]; // 计算xaxis的range长度
if(e == 1){ // msa坐标
console.log(window.layout.xaxis.range);
xstart = document.getElementById("jump_pos_start").value; // 输入感兴趣的区域在msa中起始点坐标
}else if(e == 2){ // template序列的实际坐标
xstart = document.getElementById("jump_pos_start2").value; // 输入感兴趣的区域在msa中起始点坐标
xstart = pos_from_seq_to_msa[xstart];
}else{
alert("error: not support plot_jump_to_x for"+e);
}
xstart = xstart -0.5-1;
xend = xstart + xrange_unit;
window.layout.xaxis.range = [xstart, xend];
Plotly.relayout('myDiv', window.layout);
}
function clear_msa(){
document.getElementById("msa").value = "";
}
function plot_msa(){
// read msa fasta
var msa_str = document.getElementById("msa").value;
var msa = read_fasta(msa_str);
// 定义color
var colorscale = generate_colorscale("clustal");
var the_colorscale = colorscale['config'];
//自定义template
var pseq_name_list = Object.keys(msa);
if(pseq_name_list.length == 0){
alert("error: msa is null ?");
}
var template_pid = pseq_name_list[0]; // 暂时定义第一条序列作为msa的template
var y_len = pseq_name_list.length;
var x_lens = [];
var pfa_dict = {};
var p_index = 0;
var template_seq = "";
var pseq_name_list = [];
var pos_from_seq_to_msa = {};
for([key, val] of Object.entries(msa)) {
var pid=key;
var pseq=val;
if (pid == template_pid){
if(template_seq == ""){
template_seq = pseq;
if(pfa_dict.hasOwnProperty(y_len-1)){
alert("error: y_len-1=" + y_len-1 + " had exist!")
}else{
pfa_dict[y_len-1] = pseq;
}
}else{
alert("error: get two template_pid="+template_pid+" ?");
}
}else if(!pfa_dict.hasOwnProperty(p_index)){
pfa_dict[p_index] = pseq;
pseq_name_list.push(pid);
p_index += 1;
}else{
alert("error: bug here");
}
if (! x_lens.includes(pseq.length)){
x_lens.push(pseq.length);
}
}
// 检查msa序列长度是否一致
if(x_lens.length != 1){
console.log(msa);
console.log(x_lens);
alert("error: x_lens.length="+x_lens.length);
}
pseq_name_list.push(template_pid); // template_pid 作为msa matrix的第一行！
var ytickval = [...Array(y_len).keys()];
// msa每个位点的top频率
consensus_maxfreq_list = [];
if(template_seq==""){
alert("error: template_seq is null!");
}
// 定义 pos_from_seq_to_msa
for(var i=0; i< template_seq.length; i++){
var real_i = template_seq.slice(0,i+1).replaceAll("-", "").length
if(pos_from_seq_to_msa.hasOwnProperty(real_i)){continue}
pos_from_seq_to_msa[real_i] = i;
}
var msapos = [...Array(template_seq.length).keys()];
for (var i=0; i < msapos.length; i++){
var base_column_dict = {};
var total = y_len;
var max_count = 0;
ytickval.forEach((j) => {
var base = pfa_dict[j][i];
if(base == "-"){return }; // return in ForEach mean continue!
if(! base_column_dict.hasOwnProperty(base)){
base_column_dict[base] = 0;
};
base_column_dict[base] +=1;
if(base_column_dict[base] > max_count){
max_count = base_column_dict[base];
};
});
consensus_maxfreq_list.push(max_count/total);
}
// msa matrix中每个cell对应的z值(for heatmap color)
var xlist = [];
var ylist = [];
var zlist = [];
var text_list = [];
var template_x_list = [];
ytickval.forEach((y) => {
var z_line = [];
msapos.forEach((x) => {
xlist.push(x);
ylist.push(y);
var text = pfa_dict[y][x];
text_list.push(text);
var z = get_z_for_colorscale(text, colorscale);
z_line.push(z);
var template_x = get_template_x(x, template_seq);
template_x_list.push(template_x);
});
zlist.push(z_line);
});
// output
var res = {};
res['x'] = xlist;
res['y'] = ylist;
res['z'] = zlist;
res['text'] = text_list;
res['template_x'] = template_x_list;
res['msa_len'] = x_lens[0];
res['the_colorscale'] = the_colorscale;
res['consensus_maxfreq_list'] = consensus_maxfreq_list;
res['pseq_name_list'] = pseq_name_list;
res['ytickval'] = ytickval;
res['template_pid'] = template_pid;
res['pos_from_seq_to_msa'] = pos_from_seq_to_msa;
window.pos_from_seq_to_msa = pos_from_seq_to_msa;
console.log("res:");
console.log(res);
// start plot now!
window.data, window.layout = plot_msa_by_plotlyjs(res);
console.log("msa:");
console.log(msa);
}
function get_template_x(x, template_seq){ // x is 0-based
return template_seq.slice(0, x+1).replaceAll("-", "").length ;
}
function get_z_for_colorscale(text, colorscale){
if (text == "-"){ text = "Gap"};
if(! colorscale['z'].hasOwnProperty(text)){
alert("error: text="+text+" not in colorscale"+colorscale);
//sys.exit(1)
}
return colorscale['z'][text];
}
function generate_colorscale(scheme){
if(scheme == "clustal"){
var the_colors = {
"A": 'orange',
"B": '#fff',
"C": 'green',
"D": 'red',
"E": 'red',
"F": 'blue',
"G": 'orange',
"H": 'red',
"I": 'green',
"J": '#fff',
"K": 'red',
"L": 'green',
"M": 'green',
"N": '#fff',
"O": '#fff',
"P": 'orange',
"Q": '#fff',
"R": 'red',
"S": 'orange',
"T": 'orange',
"U": '#fff',
"V": 'green',
"W": 'blue',
"X": '#fff',
"Y": 'blue',
"Z": '#fff',
"Gap": '#fff'
};
var colorscale = {};
colorscale['z'] = {};
colorscale['config'] = [];
var col_keys = Object.keys(the_colors);
var color_size = col_keys.length;
var col_index = 0;
var rgs = region_by_n(0, 1 ,color_size);
rgs.forEach((e)=>{
var start = e[0];
var end=e[1];
var z = (start+end)/2;
var base = col_keys[col_index];
colorscale['z'][base] = z;
var col = the_colors[base];
colorscale['config'].push([start, col]);
colorscale['config'].push([end, col]);
col_index ++;
});
}else{
alert("error: not support scheme="+scheme+" !");
}
return colorscale;
}
function region_by_n(s,e,n){
var res = [];
var step = (e-s)/n;
for(var i=0; i< n; i++){
var start = i * step;
var end = (i +1) * step;
res.push([start,end]);
}
return res;
}
function xx(){
var n = 250;
var t = 5;
var x = [];
var xx = [];
var y = [];
var z = [];
var text = [];
var arr = ["A","T","G", "C"];
for (var j = 0; j < t; j++){
ztemp = [];
for (var i = 0; i < n; i++) {
x.push(i);
y.push(j);
//ztemp.push(Math.floor(Math.random() * 10));
var base = arr[Math.floor(Math.random() * 4)];
text.push(base);
xx.push(i+"xx");
var base_colorvalue = 0;
if(base == "A"){
base_colorvalue = 0;
}else if(base== "T"){
base_colorvalue = 0.3;
}else if(base == "C"){
base_colorvalue=0.5;
}else{
base_colorvalue = 1;
}
ztemp.push(base_colorvalue);
}
z.push(ztemp);
}
}
function read_fasta(lines){
var res = {};
//var lines="\n>c1\nATCT\nACASTE\n>c2\nsdtattt\nsd\nstat\n>c3\ndat\nsdtsdt\ndstst\n\n";
var lines_arr = lines.replace(/^\s*/, "").split(/\n>/); // ['>c1\nATCT\nACASTE', 'c2\nsdtattt\nsd\nstatn>c3\ndat\nsdtsdt\ndstst\n\n']
if(! lines_arr[0].split(/\n/)[0].match(/^>.*/)){
alert("error: should start with > "+lines_arr[0]);
return res;
}else{
lines_arr[0] = lines_arr[0].replace(/^>/, "");
}
for(var i=0; i < lines_arr.length; i++){
var e = lines_arr[i];
var e_arr = e.split(/\n/);
//console.log(e_arr);
if(e_arr.length < 2){
console.log("skip "+e);
continue;
}
var cid=e_arr[0];
var cseq=e_arr.slice(1).join("").replace(/\s/g, "");
if(!( cid.match(/.*\S.*/) )){
alert("error:"+e);
return res;
}
if(!( cseq.match(/\S+/) )){
alert("error:"+e);
return res;
}
res[cid] = cseq;
}
//console.log(res);
return res;
}
function plot_msa_by_plotlyjs(res){
//var jsonResult="{'x': [1,2,3,1,2,3], 'y':['A','M','W','X','N','W'], 'z':[0.1,0.1,0.8,0.1,0.1,0.1], 'template_x':['1','2','3','1','2','3']}";
//var res = JSON.parse(jsonResult);
//var res={'msa_len': 200, 'x': [0, 1, 2, 0, 1, 2], y: [0, 0, 0, 1, 1, 1],
//'z': [[0, 0.2, 0.4], [0.6, 0.8, 1]],
//'text': ['A', 'M', 'W', 'X', 'N', 'W'],
//'template_x': ['1', '2', '3', '1', '2', '3']};
//var res = {'x': [0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598, 599, 600, 601, 602, 603, 604, 605, 606, 607, 608, 609, 610, 611, 612, 613, 614, 615, 616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 668, 669, 670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683, 684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729, 730, 731, 732, 733, 734, 735, 736, 737, 738, 739, 740, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767, 768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779, 780, 781, 782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794, 795, 796, 797, 798, 799, 800, 801, 802, 803, 804, 805, 806, 807, 808, 809, 810, 811, 812, 813, 814, 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828, 829, 830, 831, 832, 833, 834, 835, 836, 837, 838, 839, 840, 841, 842, 843, 844, 845, 846, 847, 848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861, 862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875, 876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889, 890, 891, 892, 893, 894, 895, 896, 897, 898, 899, 900, 901, 902, 903, 904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915, 916, 917, 918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928, 929, 930, 931, 932, 933, 934, 935, 936, 937, 938, 939, 940, 941, 942, 943, 944, 945, 946, 947, 948, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959, 960, 961, 962, 963, 964, 965, 966, 967, 968, 969, 970, 971, 972, 973, 974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987, 988, 989, 990, 991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013, 1014, 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659, 660, 661, 662, 663, 664, 665, 666, 667, 668, 669, 670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683, 684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 717, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729, 730, 731, 732, 733, 734, 735, 736, 737, 738, 739, 740, 741, 741, 741, 741, 741, 741, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767, 768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 777, 777, 778, 779, 780, 781, 782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794, 795, 796, 797, 798, 799, 800, 801, 802, 803, 804, 805, 806, 807, 808, 809, 810, 811, 812, 813, 814, 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828, 829, 830, 831, 832, 833, 834, 835, 836, 836, 836, 836, 836, 836, 836, 836, 837, 838, 839, 840, 840, 840, 841, 842, 843, 844, 845, 846, 847, 848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861, 862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875, 876, 877, 878, 879, 880, 880, 880, 880, 880, 881, 882, 883, 884, 885, 885, 886, 887, 888, 889, 890, 891, 892, 893, 893, 893, 893, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 19, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 41, 41, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 68, 69, 70, 71, 72, 72, 73, 74, 74, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 150, 150, 150, 150, 151, 152, 153, 153, 154, 155, 156, 157, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 173, 173, 173, 173, 173, 173, 173, 173, 173, 173, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 184, 184, 184, 184, 184, 184, 184, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 195, 195, 195, 195, 196, 197, 198, 199, 200, 201, 201, 201, 201, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 231, 231, 231, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 315, 316, 317, 318, 319, 320, 321, 322, 323, 323, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 340, 341, 342, 343, 344, 345, 346, 347, 348, 348, 349, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 386, 386, 386, 386, 386, 386, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 405, 405, 406, 407, 408, 408, 409, 410, 411, 412, 413, 413, 413, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 464, 464, 464, 464, 464, 464, 464, 464, 465, 466, 467, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 547, 547, 548, 549, 550, 551, 552, 553, 554, 555, 555, 555, 555, 555, 555, 556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598, 599, 600, 601, 602, 602, 602, 602, 602, 602, 602, 602, 602, 602, 602, 603, 604, 604, 605, 606, 607, 608, 609, 610, 611, 611, 611, 611, 612, 613, 614, 615, 616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 668, 669, 670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683, 684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 717, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729, 730, 731, 732, 733, 734, 735, 736, 737, 738, 739, 740, 741, 741, 741, 741, 741, 741, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767, 768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 777, 777, 778, 779, 780, 781, 782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794, 795, 796, 797, 798, 799, 800, 801, 802, 803, 804, 805, 806, 807, 808, 809, 810, 811, 812, 813, 814, 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828, 829, 830, 831, 832, 833, 834, 835, 836, 836, 836, 836, 836, 836, 836, 836, 837, 838, 839, 840, 840, 840, 841, 842, 843, 844, 845, 846, 847, 848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861, 862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875, 876, 877, 878, 879, 880, 880, 880, 880, 880, 881, 882, 883, 884, 885, 885, 886, 887, 888, 889, 890, 891, 892, 893, 893, 893, 893], 'msa_len': 1196};
//var res={'x': [0, 1, 2, 0, 1, 2, 0, 1, 2, 0, 1, 2, 0, 1, 2, 0, 1, 2, 0, 1, 2, 0, 1, 2, 0, 1, 2, 0, 1, 2, 0, 1, 2],
//'y': [0, 0, 0, 1, 1, 1, 2, 2, 2, 3, 3, 3, 4, 4, 4, 5, 5, 5, 6, 6, 6, 7, 7, 7, 8, 8, 8, 9, 9, 9, 10, 10, 10],
//'z': [[0.9814814814814814, 0.9814814814814814, 0.9814814814814814], [0.9814814814814814, 0.9814814814814814, 0.9814814814814814], [0.4629629629629629, 0.6851851851851851, 0.5740740740740741], [0.4629629629629629, 0.6851851851851851, 0.5740740740740741], [0.4629629629629629, 0.9814814814814814, 0.9814814814814814], [0.4629629629629629, 0.9814814814814814, 0.9814814814814814], [0.4629629629629629, 0.9814814814814814, 0.9814814814814814], [0.4629629629629629, 0.6851851851851851, 0.5740740740740741], [0.9814814814814814, 0.9814814814814814, 0.9814814814814814], [0.9814814814814814, 0.9814814814814814, 0.9814814814814814], [0.4629629629629629, 0.9814814814814814, 0.9814814814814814]],
//'text': ['-', '-', '-', '-', '-', '-', 'M', 'S', 'P', 'M', 'S', 'P', 'M', '-', '-', 'M', '-', '-', 'M', '-', '-', 'M', 'S', 'P', '-', '-', '-', '-', '-', '-', 'M', '-', '-'], 'template_x': [1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1], 'msa_len': 3};
var x = res["x"];
var y = res["y"];
var z = res["z"];
var text = res["text"];
var n = res['msa_len']; // msa每条序列长度
var template_x = res["template_x"]; // template模板的坐标
var the_colorscale = res["the_colorscale"];
var consensus_maxfreq_list = res['consensus_maxfreq_list'];
var pseq_name_list = res['pseq_name_list'];
var ytickval = res['ytickval'];
var template_pid = res['template_pid'];
//var pseq_name_max_len = 10;
var fig_left_margin = parseInt(document.getElementById("seq_name_left_margin").value); // convert string to int by parseInt
//var the_colorscale = [[0.0, 'orange'], [0.037037037037037035, 'orange'], [0.037037037037037035, '#fff'], [0.07407407407407407, '#fff'], [0.07407407407407407, 'green'], [0.1111111111111111, 'green'], [0.1111111111111111, 'red'], [0.14814814814814814, 'red'], [0.14814814814814814, 'red'], [0.18518518518518517, 'red'], [0.18518518518518517, 'blue'], [0.2222222222222222, 'blue'], [0.2222222222222222, 'orange'], [0.25925925925925924, 'orange'], [0.25925925925925924, 'red'], [0.2962962962962963, 'red'], [0.2962962962962963, 'green'], [0.3333333333333333, 'green'], [0.3333333333333333, '#fff'], [0.37037037037037035, '#fff'], [0.37037037037037035, 'red'], [0.4074074074074074, 'red'], [0.4074074074074074, 'green'], [0.4444444444444444, 'green'], [0.4444444444444444, 'green'], [0.48148148148148145, 'green'], [0.48148148148148145, '#fff'], [0.5185185185185185, '#fff'], [0.5185185185185185, '#fff'], [0.5555555555555556, '#fff'], [0.5555555555555556, 'orange'], [0.5925925925925926, 'orange'], [0.5925925925925926, '#fff'], [0.6296296296296295, '#fff'], [0.6296296296296295, 'red'], [0.6666666666666665, 'red'], [0.6666666666666666, 'orange'], [0.7037037037037037, 'orange'], [0.7037037037037037, 'orange'], [0.7407407407407407, 'orange'], [0.7407407407407407, '#fff'], [0.7777777777777777, '#fff'], [0.7777777777777777, 'green'], [0.8148148148148147, 'green'], [0.8148148148148148, 'blue'], [0.8518518518518519, 'blue'], [0.8518518518518519, '#fff'], [0.8888888888888888, '#fff'], [0.8888888888888888, 'blue'], [0.9259259259259258, 'blue'], [0.9259259259259258, '#fff'], [0.9629629629629628, '#fff'], [0.9629629629629629, '#fff'], [1.0, '#fff']];
//var the_colorscale = [[0, "orange"], [1, "orange"]];
view_block_size = 30;
var data1 = {
x: x,
y: y,
mode: "text",
text: text,
type: 'scattergl',
customdata: template_x,
hovertemplate: "<br>%{text} at <b>%{customdata}</b> of "+ template_pid +", %{x}+1 off the whole msa",
//hovertemplate: "<br>myth %{x}: %{y} %{customdata}",
//hovertemplate: "<extra>%{fullData.customdata}</extra>",
//xaxis: 'x', // 默认就是x
//yaxis: 'y', // 默认就是x
textfont: {
size: 13, //20,
family: 'Courier New, monospace', // Courier New 和 monospace都属等宽字体，优先用前者。
color: "black",
},
}
var data2 = {
z: z,
zmin: 0,
zmax: 1,
colorscale: the_colorscale,
type: "heatmap",
showscale: false, // 是否显示legend
hoverinfo: "none", // 关闭hover
opacity: 0.5, // 这个trace的整体颜色透明度，这个trace作为data1的背景。
}
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var x3 = consensus_maxfreq_list;
var data3 = {
y: x3,
ymin: 0,
ymax: 1,
//base: x3.map(function(element) { return element*-1}),
type: 'bar',
xaxis: 'x', // 默认是x。x not x3,因为和data1共有一个x
yaxis: 'y3', // 默认是y。
};
// todo: 蛋白全长水平的domain regeion 区域 或者 template蛋白的二级结构注释(来自https://hub.0z.gs/YuLab-SMU/ggmsa/issues/47)
var data4 = {
x: [0.5, 2, 3, 5, 9.5], // 把蛋白上每个domain实际的区域转化为msa中的坐标！
y: [1, 1, 1, 1, 1],
width: [2, 1, 1, 3, 6],
customdata: ["xx->yy","xx->yy","xx->yy","xx->yy","xx->yy"],
hovertemplate: "xx: %{customdata}", // 定制hover 内容
ymin: 0,
ymax: 1,
type: 'bar',
xaxis: 'x', // 默认是x。即data4和data1公用一个x轴。
yaxis: 'y4', // 默认是y。
marker:{
color: ["green", "black", "red", "yellow", "blue"],
},
opacity: 0.8, // 这个trace的整体颜色透明度。
}
layout = {
//grid: {rows: 2, columns: 1, pattern: 'independent'},
//sliders: sliders,
//height: "1600px",
margin: {l: fig_left_margin}, // 左侧(1即left)margin(px)
xaxis: {
range: [-0.5, view_block_size +0.5],
showline: false,
zeroline: false,
showgrid: false,
constraintoward: "left", // x轴左对齐
rangeslider: {
autorange: true,
//range: [0, view_block_size],
//visible: true,
},
},
yaxis: {
showline: false,
zeroline: false,
showgrid: false,
tickmode: "array",
tickvals: ytickval,
ticktext: pseq_name_list,
scaleanchor: "x", // xy比例对齐
scaleratio: 1,
domain: [0.35, 0.9],
},
xaxis3: {
constraintoward: "left", // x轴左对齐
},
yaxis3: {
domain: [0, 0.2],
},
xaxis4: {
constraintoward: "left", // x轴左对齐
},
yaxis4: {
domain: [0.92, 1],
showticklabels: false, // 隐藏y轴tick
},
}
// window.myDiv = document.getElementById('myDiv'); myDiv.on('plotly_click', function(eventdata){{ window.eventdata = eventdata}});
//data = [data2, data1, data3];
data = [data4, data3, data2, data1]; // 最后画data1，这样data1就在data2上方，不会被data2盖住。
Plotly.newPlot('myDiv', {data:data, layout:layout});
// 跳转到指定开始位置
return data, layout;
}
/*
> schemes.getScheme("taylor"); // npm install msa-colorschemes --save
{
A: '#ccff00',
R: '#0000ff',
N: '#cc00ff',
D: '#ff0000',
C: '#ffff00',
Q: '#ff00cc',
E: '#ff0066',
G: '#ff9900',
H: '#0066ff',
I: '#66ff00',
L: '#33ff00',
K: '#6600ff',
M: '#00ff00',
F: '#00ff66',
P: '#ffcc00',
S: '#ff3300',
T: '#ff6600',
W: '#00ccff',
Y: '#00ffcc',
V: '#99ff00',
B: '#fff',
X: '#fff',
Z: '#fff'
}
> schemes.getScheme("clustal");
{
A: 'orange',
B: '#fff',
C: 'green',
D: 'red',
E: 'red',
F: 'blue',
G: 'orange',
H: 'red',
I: 'green',
J: '#fff',
K: 'red',
L: 'green',
M: 'green',
N: '#fff',
O: '#fff',
P: 'orange',
Q: '#fff',
R: 'red',
S: 'orange',
T: 'orange',
U: '#fff',
V: 'green',
W: 'blue',
X: '#fff',
Y: 'blue',
Z: '#fff',
Gap: '#fff'
}
var msa=">UrCas13d-HA
-----------MAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNQT
QLSSKDNSNIQLGGVNEVNITFSSKHGFESGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGVKGSE--SH
DDFIGYLSTNNIYDVFIDPD-NSSLSDDKKANVRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDNRVSQAYKKR------VYHMLAIVGQIRQCVFHDKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEDNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISG-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYRNCIAH
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEK--YPYDVP
D---------YA--
>dUrCas13d-HA
-----------MAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNQT
QLSSKDNSNIQLGGVNEVNITFSSKHGFESGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGVKGSE--SH
DDFIGYLSTNNIYDVFIDPD-NSSLSDDKKANVRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDNRVSQAYKKR------VYHMLAIVGQIAQCVFADKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEDNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISG-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYANCIAA
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEK--YPYDVP
D---------YA--
>NLS-UrCas13d-NLS-HA
MSPKKKRKVEASAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNQT
QLSSKDNSNIQLGGVNEVNITFSSKHGFESGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGVKGSE--SH
DDFIGYLSTNNIYDVFIDPD-NSSLSDDKKANVRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDNRVSQAYKKR------VYHMLAIVGQIRQCVFHDKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEDNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISG-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYRNCIAH
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEKGSGPKKKR
K---------VAAA
>NLS-dUrCas13d-NLS-HA
MSPKKKRKVEASAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PAAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNQT
QLSSKDNSNIQLGGVNEVNITFSSKHGFESGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGVKGSE--SH
DDFIGYLSTNNIYDVFIDPD-NSSLSDDKKANVRKSLSKFN--ALLKTKRLGYFG--LE-
EPKTKDNRVSQAYKKR------VYHMLAIVGQIAQCVFADKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEDNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLDEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDIAAGESL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----KITDDRISG-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLGVKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCD-------------
------KSPNLFLKK---NERLRKCVEVDINNAD--------SS-----MTRKYANCIAA
LTVVRELKEYIGDI-------CTVDSYFSIYHYVMQRCITKRENDTKQEEKIKYE--DDL
LKNHGYT-------KDFVKALNSPFGYNIPRFKNLSIEQLFDR--NEYLTEKGSGPKKKR
K---------VAAA
>P1E0Cas13d-HA
M----------------------------EREV----------KKPP-------------
-----KKSLAKAAGLKSTFVISPQEKELAMTAFGRGNDALLQKRIVDGVVRDVAGEKQQF
QVQRQDESRFRLQNSRLADRT------------VTA--DDPLHRAETPRRQPLGAGMDQL
RRKAILEQKYFGRTF-DDNIHIQLIYNILDIHKMLAVPANHIVHTLNLLGG------YGE
TDFVGMLPAGLPYD---------KLRVVKKKNGDTVDIKADIAAYAKRPQLAYLG--AAF
YDVTPGKSKRDAARGRVKREQDVYAILSLMSLLRQFCAHDSVRIWGQNTTAALYH-LQAL
PQDMKDLLDDGWRRALGGVNDHFLDTNKVNLLTLFEYYGAETKQARVALTQDFYRFVVLK
EQKNMGFSLRRLREELLKLPDAAYLTGQEYDSVRQKLYMLLDFLLCRLYAQERADRCEEL
VSALRCALSDEEKDTVYQAEAAALWQALGDTLR--------RKLLPLLKGKKLQDKDKKK
SDELGLSRDVLDGVLFRPAQQGSRANADYFCRLMHLSTWFMDGKEINTLLTTLISKLENI
DSLRSVLES--MGLAYSFVPAYAMFDH---SRYIAGQLRVVNNIARMRKPAIGAKREMYR
AAVVLLGVDSPEAAAAITDDLLQI-------DPETGKVRPRSDSARDTGLRNFIANNVVE
SRRFTYLLRYMTPEQARVLAQNEKLIAFVLSTVPDTQLERYCRTCGR-EDIT-GRPAQIR
YLTAQIMGVRYESFTDVE-----QRGRG-DNPKKERYKALIGLYLTVLYLAVKNMVNCNA
RYVIAFYCRDRDTALYQK---EVCWYDLEE---DKKSGKQRQVEDYT-------------
------ALTRYFVSQGYLNRHACGYLRSNMNGIS--------NS-----LLTAYRNAVDH
LNAIPPLGSLCRDI-------GRVDSYFALYHYAVQQYLNGRYYRKTPREQELF---AAM
AQHRTWC-------SDLVKALNTPFGYNLARYKNLSIDGLFDREGDHVVRED--GEKPAE
--------------
>NLS-P1E0Cas13d-NLS-HA
M-----------------SPKKKRKVEASEREV----------KKPP-------------
-----KKSLAKAAGLKSTFVISPQEKELAMTAFGRGNDALLQKRIVDGVVRDVAGEKQQF
QVQRQDESRFRLQNSRLADRT------------VTA--DDPLHRAETPRRQPLGAGMDQL
RRKAILEQKYFGRTF-DDNIHIQLIYNILDIHKMLAVPANHIVHTLNLLGG------YGE
TDFVGMLPAGLPYD---------KLRVVKKKNGDTVDIKADIAAYAKRPQLAYLG--AAF
YDVTPGKSKRDAARGRVKREQDVYAILSLMSLLRQFCAHDSVRIWGQNTTAALYH-LQAL
PQDMKDLLDDGWRRALGGVNDHFLDTNKVNLLTLFEYYGAETKQARVALTQDFYRFVVLK
EQKNMGFSLRRLREELLKLPDAAYLTGQEYDSVRQKLYMLLDFLLCRLYAQERADRCEEL
VSALRCALSDEEKDTVYQAEAAALWQALGDTLR--------RKLLPLLKGKKLQDKDKKK
SDELGLSRDVLDGVLFRPAQQGSRANADYFCRLMHLSTWFMDGKEINTLLTTLISKLENI
DSLRSVLES--MGLAYSFVPAYAMFDH---SRYIAGQLRVVNNIARMRKPAIGAKREMYR
AAVVLLGVDSPEAAAAITDDLLQI-------DPETGKVRPRSDSARDTGLRNFIANNVVE
SRRFTYLLRYMTPEQARVLAQNEKLIAFVLSTVPDTQLERYCRTCGR-EDIT-GRPAQIR
YLTAQIMGVRYESFTDVE-----QRGRG-DNPKKERYKALIGLYLTVLYLAVKNMVNCNA
RYVIAFYCRDRDTALYQK---EVCWYDLEE---DKKSGKQRQVEDYT-------------
------ALTRYFVSQGYLNRHACGYLRSNMNGIS--------NS-----LLTAYRNAVDH
LNAIPPLGSLCRDI-------GRVDSYFALYHYAVQQYLNGRYYRKTPREQELF---AAM
AQHRTWC-------SDLVKALNTPFGYNLARYKNLSIDGLFDREGDHVVRED--GEKPAE
G-SGPKKKRKVAAA
>RffCas13d-HA
M-------------KKKMSLREKREAEKQAKKAAYSAASKNTDSKPAEKKAETPKPAEII
SDNSRNKTAVKAAGLKSTII---SGDKLYMTSFGKGNAAVIEQKIDI-------NDYSFS
AM--KDTPSLEVDKAESKEISFSSHHPFVKNDKLTT--YNPLYGGKDNPEKP--VGRDML
GLKDKLEERYFGCTF-NDNLHIQIIYNILDIEKILAVHSANITTALDHMVD-EDDEKYLN
SDYIGYMNTINTYDVFMDPSKNSSLSPKDRKNIDNSRAKFE--KLLSTKRLGYFG--FDY
DANGKDKKKNEEIKKR------LYHLTAFAGQLRQWSFHSAGN----YPRTWLYK-LDSL
DKEYLDTLDHYFDKRFNDINDDFVTKNATNLYILKEVFPEANFKDIADL---YYDFIVIK
SHKNMGFSIKKLREKMLECDGADRIKEQDMDSVRSKLYKLIDFCIF-KYYHEFPELSEKN
VDILRAAVSDTKKDNLYSDEAARLWSIFKEKFL-GFCDKI---VVW-VTGEHEKDITSVI
DK----------------DAYRNRSNVSYFSKLMYAMCFFLDGKEINDLLTTLINKFDNI
ANQIKTAKE--LGINTAFVKNYDFFNH---SEKYVDELNIVKNIARMKKPSSNAKKAMYH
DALTILGIPEDMDE-KALDEELDL-ILEKKTDPVTGKPLKGKN-----PLRNFIANNVIE
NSRFIYLIKFCNPENVRKIVNNTKVTEFVLKRIPDAQIERYYKSCTD-SEMNPPTEKKIT
ELAGKLKDMNFGNFRNVR-----QSAK--ENMEKERFKAVIGLYLTVVYRVVKNLVDVNS
RYIMAFHSLERDSQLY--------NVSVDN---DYLALTDTLVKEGD-------------
------NSRSRYLAG---NKRLRDCVKQDIDNAKKWFVSDKYNS-----IT-KYRNNVAH
LTAVRNCAEFIGDI-------TKIDSYFALYHYLIQRQLAKGLDHERSGFDRNYPQYAPL
FKWHTYV-------KDVVKALNAPFGYNIPRFKNLSIDALFDR--NEIKKND--GEKKSD
D-------------
>NLS-RffCas13d-NLS-HA
MSPKKKRKV--EASKKKMSLREKREAEKQAKKAAYSAASKNTDSKPAEKKAETPKPAEII
SDNSRNKTAVKAAGLKSTII---SGDKLYMTSFGKGNAAVIEQKIDI-------NDYSFS
AM--KDTPSLEVDKAESKEISFSSHHPFVKNDKLTT--YNPLYGGKDNPEKP--VGRDML
GLKDKLEERYFGCTF-NDNLHIQIIYNILDIEKILAVHSANITTALDHMVD-EDDEKYLN
SDYIGYMNTINTYDVFMDPSKNSSLSPKDRKNIDNSRAKFE--KLLSTKRLGYFG--FDY
DANGKDKKKNEEIKKR------LYHLTAFAGQLRQWSFHSAGN----YPRTWLYK-LDSL
DKEYLDTLDHYFDKRFNDINDDFVTKNATNLYILKEVFPEANFKDIADL---YYDFIVIK
SHKNMGFSIKKLREKMLECDGADRIKEQDMDSVRSKLYKLIDFCIF-KYYHEFPELSEKN
VDILRAAVSDTKKDNLYSDEAARLWSIFKEKFL-GFCDKI---VVW-VTGEHEKDITSVI
DK----------------DAYRNRSNVSYFSKLMYAMCFFLDGKEINDLLTTLINKFDNI
ANQIKTAKE--LGINTAFVKNYDFFNH---SEKYVDELNIVKNIARMKKPSSNAKKAMYH
DALTILGIPEDMDE-KALDEELDL-ILEKKTDPVTGKPLKGKN-----PLRNFIANNVIE
NSRFIYLIKFCNPENVRKIVNNTKVTEFVLKRIPDAQIERYYKSCTD-SEMNPPTEKKIT
ELAGKLKDMNFGNFRNVR-----QSAK--ENMEKERFKAVIGLYLTVVYRVVKNLVDVNS
RYIMAFHSLERDSQLY--------NVSVDN---DYLALTDTLVKEGD-------------
------NSRSRYLAG---NKRLRDCVKQDIDNAKKWFVSDKYNS-----IT-KYRNNVAH
LTAVRNCAEFIGDI-------TKIDSYFALYHYLIQRQLAKGLDHERSGFDRNYPQYAPL
FKWHTYV-------KDVVKALNAPFGYNIPRFKNLSIDALFDR--NEIKKND--GEKKSD
DGSGPKKKRKVAAA
>UrCas13d
-----------MAKKNKMKPRELREAQKKARQL--KAAEINNNAAPA--IAAMPAAEVIA
PVAEKKKSSVKAAGMKSILV---SENKMYITSFGKGNSAVLEYEVDN-------NDYNKT
QLSSKDNSNIELGDVNEVNITFSSKHGFGSGVEINT--SNPTHR--SGESSP--VRGDML
GLKSELEKRFFGKTF-DDNIHIQLIYNILDIEKILAVYVTNIVYALNNMLGIKDSE--SY
DDFMGYLSARNTYEVFTHPD-KSNLSDKVKGNIKKSLSKFN--DLLKTKRLGYFG--LE-
EPKTKDTRASEAYKKR------VYHMLAIVGQIRQCVFHDKSG----AKRFDLYSFINNI
DPEYRDTLDYLVEERLKSINKDFIEGNKVNISLLIDMMKGYEADDIIRL---YYDFIVLK
SQKNLGFSIKKLREKMLEEYGF-RFKDKQYDSVRSKMYKLMDFLLFCNYYRNDVAAGEAL
VRKLRFSMTDDEKEGIYADEAAKLWGKFRNDFE-NIADHMNGDVIK-ELGKADMDFDEKI
LD----------------SEKKNASDLLYFSKMIYMLTYFLDGKEINDLLTTLISKFDNI
KEFLKIMKSSAVDVECELTAGYKLFND---SQRITNELFIVKNIASMRKPAASAKLTMFR
DALTILGIDD-----NITDDRISE-ILKLKEKG------KGIH-----GLRNFITNNVIE
SSRFVYLIKYANAQKIREVAKNEKVVMFVLGGIPDTQIERYYKSCVEFPDMNSSLEAKRS
ELARMIKNISFDDFKNVK-----QQAKGRENVAKERAKAVIGLYLTVMYLLVKNLVNVNA
RYVIAIHCLERDFGLYKEIIPELASKNLKN---DYRILSQTLCELCDDRN----------
------ESSNLFLKK---NKRLRKCVEVDINNAD--------SS-----MTRKYRNCIAH
LTVVRELKEYIGDI-------RTVDSYFSIYHYVMQRCITKRGDDTKQEEKIKYE--DDL