diff --git a/ci-operator/config/openshift/machine-api-operator/openshift-machine-api-operator-master.yaml b/ci-operator/config/openshift/machine-api-operator/openshift-machine-api-operator-master.yaml
index a10184a009ab4..40442c8d46fd7 100644
--- a/ci-operator/config/openshift/machine-api-operator/openshift-machine-api-operator-master.yaml
+++ b/ci-operator/config/openshift/machine-api-operator/openshift-machine-api-operator-master.yaml
@@ -198,6 +198,13 @@ tests:
   steps:
     cluster_profile: gcp
     workflow: openshift-e2e-gcp-libvirt
+- as: e2e-nutanix
+  cluster: build01
+  optional: true
+  skip_if_only_changed: ^docs/|\.md$|^(?:.*/)?(?:\.gitignore|OWNERS|PROJECT|LICENSE)$
+  steps:
+    cluster_profile: nutanix
+    workflow: openshift-e2e-nutanix
 - as: e2e-vsphere
   optional: true
   skip_if_only_changed: ^docs/|\.md$|^(?:.*/)?(?:\.gitignore|OWNERS|PROJECT|LICENSE)$
diff --git a/ci-operator/config/openshift/machine-api-provider-nutanix/openshift-machine-api-provider-nutanix-main.yaml b/ci-operator/config/openshift/machine-api-provider-nutanix/openshift-machine-api-provider-nutanix-main.yaml
index 3e1b5105c5ce1..d8e71cce3bfcc 100644
--- a/ci-operator/config/openshift/machine-api-provider-nutanix/openshift-machine-api-provider-nutanix-main.yaml
+++ b/ci-operator/config/openshift/machine-api-provider-nutanix/openshift-machine-api-provider-nutanix-main.yaml
@@ -42,6 +42,12 @@ tests:
   steps:
     cluster_profile: aws
     workflow: openshift-e2e-aws
+- as: e2e-nutanix
+  cluster: build01
+  optional: true
+  steps:
+    cluster_profile: nutanix
+    workflow: openshift-e2e-nutanix
 - as: unit
   commands: make test
   container:
diff --git a/ci-operator/jobs/openshift/machine-api-operator/openshift-machine-api-operator-master-presubmits.yaml b/ci-operator/jobs/openshift/machine-api-operator/openshift-machine-api-operator-master-presubmits.yaml
index 34e10f9dd5a6d..154b2a69061f8 100644
--- a/ci-operator/jobs/openshift/machine-api-operator/openshift-machine-api-operator-master-presubmits.yaml
+++ b/ci-operator/jobs/openshift/machine-api-operator/openshift-machine-api-operator-master-presubmits.yaml
@@ -1045,6 +1045,81 @@ presubmits:
         secret:
           secretName: result-aggregator
     trigger: (?m)^/test( | .* )e2e-metal-ipi-virtualmedia,?($|\s.*)
+  - agent: kubernetes
+    always_run: false
+    branches:
+    - ^master$
+    - ^master-
+    cluster: build01
+    context: ci/prow/e2e-nutanix
+    decorate: true
+    labels:
+      ci-operator.openshift.io/cloud: nutanix
+      ci-operator.openshift.io/cloud-cluster-profile: nutanix
+      ci-operator.openshift.io/cluster: build01
+      ci.openshift.io/generator: prowgen
+      pj-rehearse.openshift.io/can-be-rehearsed: "true"
+    name: pull-ci-openshift-machine-api-operator-master-e2e-nutanix
+    optional: true
+    rerun_command: /test e2e-nutanix
+    skip_if_only_changed: ^docs/|\.md$|^(?:.*/)?(?:\.gitignore|OWNERS|PROJECT|LICENSE)$
+    spec:
+      containers:
+      - args:
+        - --gcs-upload-secret=/secrets/gcs/service-account.json
+        - --image-import-pull-secret=/etc/pull-secret/.dockerconfigjson
+        - --lease-server-credentials-file=/etc/boskos/credentials
+        - --report-credentials-file=/etc/report/credentials
+        - --secret-dir=/secrets/ci-pull-credentials
+        - --secret-dir=/usr/local/e2e-nutanix-cluster-profile
+        - --target=e2e-nutanix
+        command:
+        - ci-operator
+        image: ci-operator:latest
+        imagePullPolicy: Always
+        name: ""
+        resources:
+          requests:
+            cpu: 10m
+        volumeMounts:
+        - mountPath: /etc/boskos
+          name: boskos
+          readOnly: true
+        - mountPath: /secrets/ci-pull-credentials
+          name: ci-pull-credentials
+          readOnly: true
+        - mountPath: /usr/local/e2e-nutanix-cluster-profile
+          name: cluster-profile
+        - mountPath: /secrets/gcs
+          name: gcs-credentials
+          readOnly: true
+        - mountPath: /etc/pull-secret
+          name: pull-secret
+          readOnly: true
+        - mountPath: /etc/report
+          name: result-aggregator
+          readOnly: true
+      serviceAccountName: ci-operator
+      volumes:
+      - name: boskos
+        secret:
+          items:
+          - key: credentials
+            path: credentials
+          secretName: boskos-credentials
+      - name: ci-pull-credentials
+        secret:
+          secretName: ci-pull-credentials
+      - name: cluster-profile
+        secret:
+          secretName: cluster-secrets-nutanix
+      - name: pull-secret
+        secret:
+          secretName: registry-pull-credentials
+      - name: result-aggregator
+        secret:
+          secretName: result-aggregator
+    trigger: (?m)^/test( | .* )e2e-nutanix,?($|\s.*)
   - agent: kubernetes
     always_run: false
     branches:
diff --git a/ci-operator/jobs/openshift/machine-api-provider-nutanix/openshift-machine-api-provider-nutanix-main-presubmits.yaml b/ci-operator/jobs/openshift/machine-api-provider-nutanix/openshift-machine-api-provider-nutanix-main-presubmits.yaml
index 6572db55f6c28..682fcf602e318 100644
--- a/ci-operator/jobs/openshift/machine-api-provider-nutanix/openshift-machine-api-provider-nutanix-main-presubmits.yaml
+++ b/ci-operator/jobs/openshift/machine-api-provider-nutanix/openshift-machine-api-provider-nutanix-main-presubmits.yaml
@@ -72,6 +72,80 @@ presubmits:
         secret:
           secretName: result-aggregator
     trigger: (?m)^/test( | .* )e2e-aws,?($|\s.*)
+  - agent: kubernetes
+    always_run: true
+    branches:
+    - ^main$
+    - ^main-
+    cluster: build01
+    context: ci/prow/e2e-nutanix
+    decorate: true
+    labels:
+      ci-operator.openshift.io/cloud: nutanix
+      ci-operator.openshift.io/cloud-cluster-profile: nutanix
+      ci-operator.openshift.io/cluster: build01
+      ci.openshift.io/generator: prowgen
+      pj-rehearse.openshift.io/can-be-rehearsed: "true"
+    name: pull-ci-openshift-machine-api-provider-nutanix-main-e2e-nutanix
+    optional: true
+    rerun_command: /test e2e-nutanix
+    spec:
+      containers:
+      - args:
+        - --gcs-upload-secret=/secrets/gcs/service-account.json
+        - --image-import-pull-secret=/etc/pull-secret/.dockerconfigjson
+        - --lease-server-credentials-file=/etc/boskos/credentials
+        - --report-credentials-file=/etc/report/credentials
+        - --secret-dir=/secrets/ci-pull-credentials
+        - --secret-dir=/usr/local/e2e-nutanix-cluster-profile
+        - --target=e2e-nutanix
+        command:
+        - ci-operator
+        image: ci-operator:latest
+        imagePullPolicy: Always
+        name: ""
+        resources:
+          requests:
+            cpu: 10m
+        volumeMounts:
+        - mountPath: /etc/boskos
+          name: boskos
+          readOnly: true
+        - mountPath: /secrets/ci-pull-credentials
+          name: ci-pull-credentials
+          readOnly: true
+        - mountPath: /usr/local/e2e-nutanix-cluster-profile
+          name: cluster-profile
+        - mountPath: /secrets/gcs
+          name: gcs-credentials
+          readOnly: true
+        - mountPath: /etc/pull-secret
+          name: pull-secret
+          readOnly: true
+        - mountPath: /etc/report
+          name: result-aggregator
+          readOnly: true
+      serviceAccountName: ci-operator
+      volumes:
+      - name: boskos
+        secret:
+          items:
+          - key: credentials
+            path: credentials
+          secretName: boskos-credentials
+      - name: ci-pull-credentials
+        secret:
+          secretName: ci-pull-credentials
+      - name: cluster-profile
+        secret:
+          secretName: cluster-secrets-nutanix
+      - name: pull-secret
+        secret:
+          secretName: registry-pull-credentials
+      - name: result-aggregator
+        secret:
+          secretName: result-aggregator
+    trigger: (?m)^/test( | .* )e2e-nutanix,?($|\s.*)
   - agent: kubernetes
     always_run: true
     branches: