diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 327eb0fcc28..cbd999680f6 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -16,7 +16,7 @@ env:
   # backwards compatibility with the previous two minor releases and we
   # explicitly test our code for these versions so keeping this at prior
   # versions does not add value.
-  DEFAULT_GO_VERSION: "~1.24.0"
+  DEFAULT_GO_VERSION: "~1.25.0"
 jobs:
   lint:
     runs-on: ubuntu-latest
@@ -102,7 +102,7 @@ jobs:
   compatibility-test:
     strategy:
       matrix:
-        go-version: ["1.24.0", "1.23.0"]
+        go-version: ["1.25.0", "1.24.0", "1.23.0"]
         platform:
           - os: ubuntu-latest
             arch: "386"
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8484e61fe34..a18d80a66f4 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -8,6 +8,9 @@ This project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.htm
 
 ## [Unreleased]
 
+This release is the last to support [Go 1.23].
+The next release will require at least [Go 1.24].
+
 ### Added
 
 - Add the `WithTraceAttributeFn` option to `go.opentelemetry.io/contrib/instrumentation/github.com/aws/aws-lambda-go/otellambda`. (#7556)
@@ -16,6 +19,7 @@ This project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.htm
 - The `go.opentelemetry.io/contrib/detectors/autodetect` package is added to automatically compose user defined `resource.Detector`s at runtime. (#7522)
 - Add the `WithLoggerProviderOptions`, `WithMeterProviderOptions` and `WithTracerProviderOptions` options to `NewSDK` to allow passing custom options to providers in `go.opentelemetry.io/contrib/otelconf`. (#7552)
 - Added V2 version of AWS EC2 detector `go.opentelemetry.io/contrib/detectors/aws/ec2/v2` due to deprecation of `github.com/aws/aws-sdk-go`. (#6961)
+- Support testing of [Go 1.25]. (#7732)
 
 ### Changed
 
@@ -1572,6 +1576,7 @@ First official tagged release of `contrib` repository.
 
 <!-- Released section ended -->
 
+[Go 1.25]: https://go.dev/doc/go1.25
 [Go 1.24]: https://go.dev/doc/go1.24
 [Go 1.23]: https://go.dev/doc/go1.23
 [Go 1.22]: https://go.dev/doc/go1.22
diff --git a/README.md b/README.md
index a634df44574..3b377e6720b 100644
--- a/README.md
+++ b/README.md
@@ -52,16 +52,22 @@ This project is tested on the following systems.
 
 | OS       | Go Version | Architecture |
 | -------- | ---------- | ------------ |
+| Ubuntu   | 1.25       | amd64        |
 | Ubuntu   | 1.24       | amd64        |
 | Ubuntu   | 1.23       | amd64        |
+| Ubuntu   | 1.25       | 386          |
 | Ubuntu   | 1.24       | 386          |
 | Ubuntu   | 1.23       | 386          |
+| macOS 13 | 1.25       | amd64        |
 | macOS 13 | 1.24       | amd64        |
 | macOS 13 | 1.23       | amd64        |
+| macOS    | 1.25       | arm64        |
 | macOS    | 1.24       | arm64        |
 | macOS    | 1.23       | arm64        |
+| Windows  | 1.25       | amd64        |
 | Windows  | 1.24       | amd64        |
 | Windows  | 1.23       | amd64        |
+| Windows  | 1.25       | 386          |
 | Windows  | 1.24       | 386          |
 | Windows  | 1.23       | 386          |
 
diff --git a/instrumentation/github.com/aws/aws-lambda-go/otellambda/example/Dockerfile b/instrumentation/github.com/aws/aws-lambda-go/otellambda/example/Dockerfile
index 065304d741a..7902d58d7e5 100644
--- a/instrumentation/github.com/aws/aws-lambda-go/otellambda/example/Dockerfile
+++ b/instrumentation/github.com/aws/aws-lambda-go/otellambda/example/Dockerfile
@@ -1,6 +1,6 @@
 # Copyright The OpenTelemetry Authors
 # SPDX-License-Identifier: Apache-2.0
-FROM golang:1.24 AS base
+FROM golang:1.25 AS base
 COPY . /src/
 WORKDIR /src/instrumentation/github.com/aws/aws-lambda-go/otellambda/example
 RUN apt-get update
diff --git a/instrumentation/github.com/aws/aws-sdk-go-v2/otelaws/example/Dockerfile b/instrumentation/github.com/aws/aws-sdk-go-v2/otelaws/example/Dockerfile
index 1a0fe7925d2..bca57e54705 100644
--- a/instrumentation/github.com/aws/aws-sdk-go-v2/otelaws/example/Dockerfile
+++ b/instrumentation/github.com/aws/aws-sdk-go-v2/otelaws/example/Dockerfile
@@ -1,6 +1,6 @@
 # Copyright The OpenTelemetry Authors
 # SPDX-License-Identifier: Apache-2.0
-FROM golang:1.24-alpine AS base
+FROM golang:1.25-alpine AS base
 COPY . /src/
 WORKDIR /src/instrumentation/github.com/aws/aws-sdk-go-v2/otelaws/example
 
diff --git a/instrumentation/net/http/httptrace/otelhttptrace/example/Dockerfile b/instrumentation/net/http/httptrace/otelhttptrace/example/Dockerfile
index d317887ffd3..5cbd3a43d63 100644
--- a/instrumentation/net/http/httptrace/otelhttptrace/example/Dockerfile
+++ b/instrumentation/net/http/httptrace/otelhttptrace/example/Dockerfile
@@ -1,6 +1,6 @@
 # Copyright The OpenTelemetry Authors
 # SPDX-License-Identifier: Apache-2.0
-FROM golang:1.24-alpine AS base
+FROM golang:1.25-alpine AS base
 COPY . /src/
 WORKDIR /src/instrumentation/net/http/httptrace/otelhttptrace/example
 
diff --git a/instrumentation/net/http/otelhttp/example/Dockerfile b/instrumentation/net/http/otelhttp/example/Dockerfile
index f41a716dd40..07c91bfd9dc 100644
--- a/instrumentation/net/http/otelhttp/example/Dockerfile
+++ b/instrumentation/net/http/otelhttp/example/Dockerfile
@@ -1,6 +1,6 @@
 # Copyright The OpenTelemetry Authors
 # SPDX-License-Identifier: Apache-2.0
-FROM golang:1.24-alpine AS base
+FROM golang:1.25-alpine AS base
 COPY . /src/
 WORKDIR /src/instrumentation/net/http/otelhttp/example