diff --git a/tooling/noir_js/scripts/compile_test_programs.sh b/tooling/noir_js/scripts/compile_test_programs.sh
index 738db98bc06..9776a0a5020 100755
--- a/tooling/noir_js/scripts/compile_test_programs.sh
+++ b/tooling/noir_js/scripts/compile_test_programs.sh
@@ -8,3 +8,5 @@ $NARGO --program-dir ./test/noir_compiled_examples/assert_msg_runtime compile --
 $NARGO --program-dir ./test/noir_compiled_examples/fold_fibonacci compile --force --pedantic-solving
 $NARGO --program-dir ./test/noir_compiled_examples/assert_raw_payload compile --force --pedantic-solving
 $NARGO --program-dir ./test/noir_compiled_examples/databus compile --force --pedantic-solving
+# Compile with no inlining to test runtime call stacks
+$NARGO --program-dir ./test/noir_compiled_examples/assert_inside_brillig_nested compile --force --pedantic-solving --inliner-aggressiveness -9223372036854775808
\ No newline at end of file
diff --git a/tooling/noir_js/src/debug.ts b/tooling/noir_js/src/debug.ts
index 4f915071388..7003bd4abbe 100644
--- a/tooling/noir_js/src/debug.ts
+++ b/tooling/noir_js/src/debug.ts
@@ -71,13 +71,13 @@ function resolveOpcodeLocations(
   );
   // Adds the acir call stack if the last location is a brillig opcode
   if (locations.length > 0) {
-    const runtimeLocations = opcodeLocations[opcodeLocations.length - 1].split('.');
-    if (runtimeLocations.length === 2) {
-      const acirCallstackId = debug.acir_locations[runtimeLocations[0]];
+    const decomposedOpcodeLocation = opcodeLocations[opcodeLocations.length - 1].split('.');
+    if (decomposedOpcodeLocation.length === 2) {
+      const acirCallstackId = debug.acir_locations[decomposedOpcodeLocation[0]];
       if (acirCallstackId !== undefined) {
         const callStack = debug.location_tree.locations[acirCallstackId];
         const acirCallstack = getCallStackFromLocationNode(callStack, debug.location_tree.locations, files);
-        locations = locations.concat(acirCallstack);
+        locations = acirCallstack.concat(locations);
       }
     }
   }
@@ -110,7 +110,8 @@ function getCallStackFromLocationNode(
 
     callStack = location_tree[callStack.parent];
   }
-  return result;
+  // Reverse since we explored the child nodes first
+  return result.reverse();
 }
 /**
  * Extracts the call stack from the location of a failing opcode and the debug metadata.
diff --git a/tooling/noir_js/test/node/execute.test.ts b/tooling/noir_js/test/node/execute.test.ts
index f657adefd95..23f7e2d4a24 100644
--- a/tooling/noir_js/test/node/execute.test.ts
+++ b/tooling/noir_js/test/node/execute.test.ts
@@ -3,6 +3,7 @@ import assert_msg_json from '../noir_compiled_examples/assert_msg_runtime/target
 import fold_fibonacci_json from '../noir_compiled_examples/fold_fibonacci/target/fold_fibonacci.json' assert { type: 'json' };
 import assert_raw_payload_json from '../noir_compiled_examples/assert_raw_payload/target/assert_raw_payload.json' assert { type: 'json' };
 import databus_json from '../noir_compiled_examples/databus/target/databus.json' assert { type: 'json' };
+import assert_inside_brillig_nested_json from '../noir_compiled_examples/assert_inside_brillig_nested/target/assert_inside_brillig_nested.json' assert { type: 'json' };
 
 import { Noir, ErrorWithPayload } from '@noir-lang/noir_js';
 import { CompiledCircuit } from '@noir-lang/types';
@@ -12,6 +13,7 @@ const assert_lt_program = assert_lt_json as CompiledCircuit;
 const assert_msg_runtime = assert_msg_json as CompiledCircuit;
 const fold_fibonacci_program = fold_fibonacci_json as CompiledCircuit;
 const databus_program = databus_json as CompiledCircuit;
+const assert_inside_brillig_nested = assert_inside_brillig_nested_json as CompiledCircuit;
 
 it('executes a single-ACIR program correctly', async () => {
   const inputs = {
@@ -40,8 +42,38 @@ it('circuit with a fmt string assert message should fail with the resolved asser
   } catch (error) {
     const knownError = error as ErrorWithPayload;
     expect(knownError.message).to.equal('Circuit execution failed: Expected x < y but got 10 < 5');
-    expect(knownError.noirCallStack).to.have.lengthOf(1);
-    expect(knownError.noirCallStack![0]).to.match(/^at x < y \(.*assert_msg_runtime\/src\/main.nr:3:12\)$/);
+  }
+});
+
+it('circuit with a nested assertion should fail with the resolved call stack', async () => {
+  try {
+    await new Noir(assert_msg_runtime).execute({
+      x: '10',
+      y: '5',
+    });
+  } catch (error) {
+    const knownError = error as ErrorWithPayload;
+    expect(knownError.noirCallStack).to.have.lengthOf(2);
+    expect(knownError.noirCallStack![0]).to.match(
+      /^at make_assertion\(x, y\) \(.*assert_msg_runtime\/src\/main.nr:2:5\)$/,
+    );
+    expect(knownError.noirCallStack![1]).to.match(/^at x < y \(.*assert_msg_runtime\/src\/main.nr:7:12\)$/);
+  }
+});
+
+it('circuit with a nested assertion inside brillig should fail with the resolved call stack', async () => {
+  try {
+    await new Noir(assert_inside_brillig_nested).execute({
+      x: '10',
+    });
+  } catch (error) {
+    const knownError = error as ErrorWithPayload;
+    const expectedStack = ['acir_wrapper(x)', 'brillig_entrypoint(x)', 'brillig_nested(x)', 'x < 10'];
+    expect(knownError.noirCallStack).to.have.lengthOf(expectedStack.length);
+
+    for (let i = 0; i < expectedStack.length; i++) {
+      expect(knownError.noirCallStack![i]).to.contain(expectedStack[i]);
+    }
   }
 });
 
diff --git a/tooling/noir_js/test/noir_compiled_examples/assert_inside_brillig_nested/Nargo.toml b/tooling/noir_js/test/noir_compiled_examples/assert_inside_brillig_nested/Nargo.toml
new file mode 100644
index 00000000000..a96be316b2f
--- /dev/null
+++ b/tooling/noir_js/test/noir_compiled_examples/assert_inside_brillig_nested/Nargo.toml
@@ -0,0 +1,5 @@
+[package]
+name = "assert_inside_brillig_nested"
+type = "bin"
+authors = [""]
+[dependencies]
diff --git a/tooling/noir_js/test/noir_compiled_examples/assert_inside_brillig_nested/src/main.nr b/tooling/noir_js/test/noir_compiled_examples/assert_inside_brillig_nested/src/main.nr
new file mode 100644
index 00000000000..a121ef0f8a3
--- /dev/null
+++ b/tooling/noir_js/test/noir_compiled_examples/assert_inside_brillig_nested/src/main.nr
@@ -0,0 +1,17 @@
+fn main(x: u64) {
+    acir_wrapper(x);
+}
+
+fn acir_wrapper(x: u64) {
+    unsafe {
+        brillig_entrypoint(x);
+    }
+}
+
+unconstrained fn brillig_entrypoint(x: u64) {
+    brillig_nested(x);
+}
+
+unconstrained fn brillig_nested(x: u64) {
+    assert(x < 10);
+}
diff --git a/tooling/noir_js/test/noir_compiled_examples/assert_msg_runtime/src/main.nr b/tooling/noir_js/test/noir_compiled_examples/assert_msg_runtime/src/main.nr
index 0bcd5c58c24..e9b980afa70 100644
--- a/tooling/noir_js/test/noir_compiled_examples/assert_msg_runtime/src/main.nr
+++ b/tooling/noir_js/test/noir_compiled_examples/assert_msg_runtime/src/main.nr
@@ -1,4 +1,8 @@
 fn main(x: u64, y: pub u64) {
+    make_assertion(x, y);
+}
+
+fn make_assertion(x: u64, y: u64) {
     // A fmtstr assertion message is used to show that noirJS will decode the error payload as a string.
     assert(x < y, f"Expected x < y but got {x} < {y}");
 }