diff --git a/lib/assert.js b/lib/assert.js
index b7d7a3da01d520..9dfcf80a913942 100644
--- a/lib/assert.js
+++ b/lib/assert.js
@@ -73,6 +73,7 @@ const CallTracker = require('internal/assert/calltracker');
 const {
   validateFunction,
 } = require('internal/validators');
+const { fileURLToPath } = require('internal/url');
 
 let isDeepEqual;
 let isDeepStrictEqual;
@@ -296,7 +297,7 @@ function getErrMessage(message, fn) {
   overrideStackTrace.set(err, (_, stack) => stack);
   const call = err.stack[0];
 
-  const filename = call.getFileName();
+  let filename = call.getFileName();
   const line = call.getLineNumber() - 1;
   let column = call.getColumnNumber() - 1;
   let identifier;
@@ -330,6 +331,14 @@ function getErrMessage(message, fn) {
         const { StringDecoder } = require('string_decoder');
         decoder = new StringDecoder('utf8');
       }
+
+      // ESM file prop is a file proto. Convert that to path.
+      // This ensure opensync will not throw ENOENT for ESM files.
+      const fileProtoPrefix = 'file://';
+      if (StringPrototypeStartsWith(filename, fileProtoPrefix)) {
+        filename = fileURLToPath(filename);
+      }
+
       fd = openSync(filename, 'r', 0o666);
       // Reset column and message.
       ({ 0: column, 1: message } = getCode(fd, line, column));
diff --git a/test/parallel/test-assert-esm-cjs-message-verify.js b/test/parallel/test-assert-esm-cjs-message-verify.js
new file mode 100644
index 00000000000000..9a66d83abd9c4b
--- /dev/null
+++ b/test/parallel/test-assert-esm-cjs-message-verify.js
@@ -0,0 +1,51 @@
+'use strict';
+
+const { spawnPromisified } = require('../common');
+const tmpdir = require('../common/tmpdir');
+const assert = require('assert');
+const { writeFileSync, unlink } = require('fs');
+const { describe, after, it } = require('node:test');
+
+tmpdir.refresh();
+
+const fileImports = {
+  cjs: 'const assert = require("assert");',
+  mjs: 'import assert from "assert";',
+};
+
+const fileNames = [];
+
+for (const [ext, header] of Object.entries(fileImports)) {
+  const fileName = `test-file.${ext}`;
+  // Store the generated filesnames in an array
+  fileNames.push(`${tmpdir.path}/${fileName}`);
+
+  writeFileSync(tmpdir.resolve(fileName), `${header}\nassert.ok(0 === 2);`);
+}
+
+describe('ensure the assert.ok throwing similar error messages for esm and cjs files', () => {
+  const nodejsPath = `${process.execPath}`;
+  const errorsMessages = [];
+
+  it('should return code 1 for each command', async () => {
+    for (const fileName of fileNames) {
+      const { stderr, code } = await spawnPromisified(nodejsPath, [fileName]);
+      assert.strictEqual(code, 1);
+      // For each error message, filter the lines which will starts with AssertionError
+      errorsMessages.push(
+        stderr.split('\n').find((s) => s.startsWith('AssertionError'))
+      );
+    }
+  });
+
+  after(() => {
+    assert.strictEqual(errorsMessages.length, 2);
+    assert.deepStrictEqual(errorsMessages[0], errorsMessages[1]);
+
+    for (const fileName of fileNames) {
+      unlink(fileName, () => {});
+    }
+
+    tmpdir.refresh();
+  });
+});