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Currently, the pipeline generates three SummarizedExperiment .rds files while it would be more convenient and less confusing to generate a single one with different assay layers.
Current situation
There are three rds files:
All of which have a counts and abundance layer
The abundance layer is the same in all three objects
While the counts are different representing counts, length scaled counts and scaled counts, respectively.
Proposed solution
Create a single SummarizedExperiment with the following assays:
tpm (what is currently in "abundance", but the name is more explicit)
counts
counts_scaled
counts_length_scaled
gene_lengths (what is currently in salmon.merged.gene_lengths.tsv)
possibly, it could be worth adding a vst layer generated by DESeq2::vst().
Create another one for the transcripts with the same layers.
The text was updated successfully, but these errors were encountered:
Description of feature
Currently, the pipeline generates three SummarizedExperiment
.rds
files while it would be more convenient and less confusing to generate a single one with differentassay
layers.Current situation
There are three rds files:
All of which have a
counts
andabundance
layerThe abundance layer is the same in all three objects
While the
counts
are different representing counts, length scaled counts and scaled counts, respectively.Proposed solution
Create a single SummarizedExperiment with the following assays:
tpm
(what is currently in "abundance", but the name is more explicit)counts
counts_scaled
counts_length_scaled
gene_lengths
(what is currently insalmon.merged.gene_lengths.tsv
)possibly, it could be worth adding a
vst
layer generated byDESeq2::vst()
.Create another one for the transcripts with the same layers.
The text was updated successfully, but these errors were encountered: