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Unify SummarizedExperiment output #1465

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grst opened this issue Dec 10, 2024 · 1 comment
Open

Unify SummarizedExperiment output #1465

grst opened this issue Dec 10, 2024 · 1 comment

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@grst
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grst commented Dec 10, 2024

Description of feature

Currently, the pipeline generates three SummarizedExperiment .rds files while it would be more convenient and less confusing to generate a single one with different assay layers.

Current situation

There are three rds files:
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All of which have a counts and abundance layer
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The abundance layer is the same in all three objects

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While the counts are different representing counts, length scaled counts and scaled counts, respectively.

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Proposed solution

Create a single SummarizedExperiment with the following assays:

  • tpm (what is currently in "abundance", but the name is more explicit)
  • counts
  • counts_scaled
  • counts_length_scaled
  • gene_lengths (what is currently in salmon.merged.gene_lengths.tsv)

possibly, it could be worth adding a vst layer generated by DESeq2::vst().

Create another one for the transcripts with the same layers.

@grst
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grst commented Dec 12, 2024

@drpatelh would you accept a PR for this?

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