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Fix cellranger multi handling of crispr data #7175

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@svigneau svigneau commented Dec 6, 2024

PR checklist

Closes #7174

  • This comment contains a description of changes (with reason).
  • Ensure that the test works with either Docker / Singularity. Conda CI tests can be quite flaky:
    • For modules:
      • nf-core modules test <MODULE> --profile docker
      • nf-core modules test <MODULE> --profile singularity

The proposed changes are described in issue 7174 and intended to fix a bug that prevents handling of CRISPR data when using cellranger multi with gene expression and CRISPR libraries.

The addition of a test dataset with CRISPR data has been requested in PR 1415. A dedicated test for CRISPR data handling by cellranger multi will be submitted in a separate PR once the test dataset is approved.

I removed testing using conda profile from the checklist because running it leads to the following error related to a different module: CELLRANGER_MKGTF module does not support Conda. Please use Docker / Singularity / Podman instead.

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famosab commented Dec 10, 2024

We need to add this tool to .github/conda_skip.yml then. Can you do that?

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Hi @svigneau ,
Thanks for the code work here 😄
There are few things before we can consider it done.

  1. We have to skip conda checks as highlighted by @famosab here
  2. Now that the test-datasets for crispr are available, we must also include the nf-test run for crispr. Can you add that?

Other than that, it is good.

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cellranger multi with gene expression and CRISPR data fails to process CRISPR data
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