the error of Augur

[crystal0721]

hi, we test augur using our data. the code as follows:

seurat.obj<-readRDS('zsh_subset.rds')
augur = calculate_auc(seurat.obj,cell_type_col = "stim", label_col = "seurat_clusters")

while we got the errors:

using default assay: RNA ...
Error in calculate_auc(seurat.obj, cell_type_col = "stim", label_col = "seurat_clusters") :
no cell type had at least 20 cells in all conditions
Execution halted

we want to know how to know the reason and how to solve. Thanks for your help and we are looking for your replay.

[skinnider]

As the error suggests, there are no cell types with at least 20 cells in all conditions. It seems to me from the code provided that you may be swapping the cell type and label columns in Augur's arguments.

[crystal0721]

Thanks for your apply. Yes, we swapped the cell type and label columns in Augur's arguments. While the number of cells under each condition is more than 20，one is 6413，the other is 3764.