How could I access the AUC per cell?

[sanchezy]

Hi all,

I would like to subset my Seurat object by the AUC score (to keep only the cells with higher AUC).
To add the AUC per cell into the metadata of my Seurat object, I need a list of the barcode and the corresponding AUC scores.
How could I access the AUC per cell?

Many thanks for your help!

[skinnider]

Hi @sanchezy, you could try something like this (assuming you have a Seurat object sc, cell type in column cell_type of its metadata, and Augur results augur):

sc@meta.data %<>% left_join(augur$AUC, by = 'cell_type')

[bigfacebig]

What is the recommended number of cells for calculation?